抗uPA Receptor抗体(Anti-uPA Receptor, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human uPARLeu23-Arg303Accession # Q03405
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human uPAR in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse uPAR is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Immunohistochemistry	1-15 µg/mL	See below
Immunoprecipitation	1 µg/106 cells	U937 human histiocytic lymphoma cell line, see our available Western blot detection antibodies
Blockade of Receptor-ligand Interaction	In a functional ELISA, 1-5 µg/mL of this antibody will block 50% of the binding of 10 ng/mL of Recombinant Human u-Plasminogen Activator/Urokinase (Catalog # 1310-SE) to immobilized Recombinant Human uPAR (Catalog # 807-UK) coated at 5 µg/mL (100 µL/well). At 30 μg/mL, this antibody will block >90% of the binding.

Western Blot
	Detection of Human uPAR by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line and Saos‑2 human osteosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human uPAR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF807) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for uPAR at approximately 30-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
	uPAR in Human Lung Cancer Tissue. uPAR was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Goat Anti-Human uPAR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF807) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

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Related Product & Information

Background

uPAR

background_content

Background: uPAR The urokinase-type Plasminogen Activator (uPA) is one of two activators that converts the extracellular zymogen plasminogen to plasmin, a serine protease that is involved in a variety of normal and pathological processes that require cell migration and/or tissue destruction. uPA is synthesized and released from cells as a single-chain (sc) pro-enzyme with limited enzymatic activity and is converted to an active two-chain (tc) disulfide-linked active enzyme by plasmin and other specific proteinases. Both the scuPA and tcuPA bind with high-affinity to the cell surface via the glycosyl phosphatidylinositol-linked receptor uPAR which serves to localize the uPA proteolytic activity. The enzymatic activity of scuPA has also been shown to be enhanced by binding to uPAR. Independent of their proteolytic activity, the uPA/uPAR interaction also initiates signal transduction responses resulting in activation of protein tyrosine kinases, gene expression, cell adhesion, and chemotaxis. uPAR can interact with integrins to suppress normal integrin adhesive function and promote adhesion to vitronectin through a high affinity vitronectin binding site on uPAR. uPAR cDNA encodes a 335 amino acid (aa) residue precursor protein with a 22 aa residue signal peptide, five potential N-linked glycosylation sites and a C‑terminal GPI-anchor site. An alternate spliced variant of uPAR encoding a secreted soluble form of uPAR also exists. Human and mouse uPAR share approximately 60% aa sequence identity and the receptor-ligand interaction is strictly species-specific.

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Citations

1. Integrin-uPAR signaling leads to FRA-1 phosphorylation and enhanced breast cancer invasion
   Authors: MG Annis, V Ouellet, JP Rennhack, S L'Esperanc, C Rancourt, AM Mes-Masson, ER Andrechek, PM Siegel
   Breast Cancer Res., 2018;20(1):9.
   Species: Human
   Sample Type: Protein
   Application: IP


2. Ang II-stimulated migration of vascular smooth muscle cells is dependent on LR11 in mice.
   Authors: Jiang M, Bujo H, Ohwaki K, Unoki H, Yamazaki H, Kanaki T, Shibasaki M, Azuma K, Harigaya K, Schneider WJ, Saito Y
   J. Clin. Invest., 2008;118(8):2733-46.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: IP


3. Modification of kidney barrier function by the urokinase receptor.
   Authors: Wei C, Moller CC, Altintas MM, Li J, Schwarz K, Zacchigna S, Xie L, Henger A, Schmid H, Rastaldi MP, Cowan P, Kretzler M, Parrilla R, Bendayan M, Gupta V, Nikolic B, Kalluri R, Carmeliet P, Mundel P, Reiser J
   Nat. Med., 2007;14(1):55-63.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


4. Pitavastatin attenuates the PDGF-induced LR11/uPA receptor-mediated migration of smooth muscle cells.
   Authors: Jiang M, Bujo H, Zhu Y, Yamazaki H, Hirayama S, Kanaki T, Shibasaki M, Takahashi K, Schneider WJ, Saito Y
   Biochem. Biophys. Res. Commun., 2006;348(4):1367-77.
   Species: Rabbit
   Sample Type: Cell Lysates
   Application: WB

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