抗BLC抗体(Anti-BLC, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human CXCL13/BCA-1Val23-Arg94Accession # Q53X90
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects CXCL13/BLC/BCA‑1 in direct ELISAs and Western blots.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human CXCL13/BLC/BCA‑1 (Catalog # 801-CX)
Immunohistochemistry	5-15 µg/mL	See below
Neutralization	Measured by its ability to neutralize CXCL13/BLC/BCA‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR5. The Neutralization Dose (ND50) is typically 1-4 µg/mL in the presence of 50 ng/mL Recombinant Human CXCL13/BLC/BCA‑1.

Neutralization
	Chemotaxis Induced by CXCL13/BLC/BCA‑1 and Neutralization by Human CXCL13/BLC/BCA‑1 Antibody. Recombinant Human CXCL13/BLC/BCA‑1 (Catalog # 801-CX) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Human CXCL13/BLC/BCA‑1 (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CXCL13/BLC/BCA‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF801). The ND50 is typically 1‑4 µg/mL.
Immunohistochemistry
	CXCL13/BLC/BCA‑1 in Human Lymphoma. CXCL13/BLC/BCA‑1 was detected in immersion fixed paraffin-embedded sections of human lymphoma using Goat Anti-Human CXCL13/BLC/BCA‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF801) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

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Related Product & Information

Entrez Gene IDs	10563 (Human); 55985 (Mouse)
Background	CXCL13/BLC/BCA-1
background_content	Background: CXCL13/BLC/BCA-1 CXCL13, also known as B-lymphocyte chemoattractant (BLC), is a CXC chemokine that is constitutively expressed in secondary lymphoid organs. BCA-1 cDNA encodes a protein of 109 amino acid residues with a leader sequence of 22 residues. Mature human BCA-1 shares 64% amino acid sequence similarity with the mouse protein and 23‑34% amino acid sequence identity with other known CXC chemokines. Recombinant or chemically synthesized BCA-1 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein-coupled receptor originally isolated from Burkitt’s lymphoma cells, has now been shown to be the specific receptor for BCA1. Among cells of the hematopoietic lineages, the expression of BLR1, now designated CXCR5, is restricted to B lymphocytes and a subpopulation of T helper memory cells. Mice lacking BLR1 have been shown to lack inguinal lymph nodes. These mice were also found to have impaired development of Peyer’s patches and defective formation of primary follicles and germinal centers in the spleen as a result of the inability of B lymphocytes to migrate into B cell areas.

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Citations

1. Elevated Expression of Chemokine CXCL13 in Chronic Hepatitis B Patients Links to Immune Control during Antiviral Therapy
   Authors: C Liu, X Huang, M Werner, R Broering, J Ge, Y Li, B Liao, J Sun, J Peng, M Lu, J Hou, X Zhang
   Front Immunol, 2017;8(0):323.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


2. Hif1a Deletion Reveals Pro-Neoplastic Function of B Cells in Pancreatic Neoplasia
   Cancer Discov, 2016;6(3):256-69.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC - Paraffin embedded


3. Control of the T follicular helper-germinal center B-cell axis by CD8(+) regulatory T cells limits atherosclerosis and tertiary lymphoid organ development.
   Authors: Clement M, Guedj K, Andreata F, Morvan M, Bey L, Khallou-Laschet J, Gaston A, Delbosc S, Alsac J, Bruneval P, Deschildre C, Le Borgne M, Castier Y, Kim H, Cantor H, Michel J, Caligiuri G, Nicoletti A
   Circulation, 2015;131(6):560-70.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC - Paraffin embedded


4. Occurrence of tertiary lymphoid tissue is associated with T-cell infiltration and predicts better prognosis in early-stage colorectal cancers.
   Authors: Di Caro G, Bergomas F, Grizzi F, Doni A, Bianchi P, Malesci A, Laghi L, Allavena P, Mantovani A, Marchesi F
   Clin Cancer Res, 2014;20(8):2147-58.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


5. CXCR5(+) T helper cells mediate protective immunity against tuberculosis.
   Authors: Slight S, Rangel-Moreno J, Gopal R, Lin Y, Fallert Junecko B, Mehra S, Selman M, Becerril-Villanueva E, Baquera-Heredia J, Pavon L, Kaushal D, Reinhart T, Randall T, Khader S
   J Clin Invest, 2013;123(2):712-26.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


6. Thymic alterations in GM2 gangliosidoses model mice.
   Authors: Kanzaki S, Yamaguchi A, Yamaguchi K, Kojima Y, Suzuki K, Koumitsu N, Nagashima Y, Nagahama K, Ehara M, Hirayasu Y, Ryo A, Aoki I, Yamanaka S
   PLoS ONE, 2010;5(8):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


7. Mature antigen-experienced T helper cells synthesize and secrete the B cell chemoattractant CXCL13 in the inflammatory environment of the rheumatoid joint.
   Arthritis Rheum., 2008;58(0):3377-87.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


8. Interaction of the Lyme disease spirochete Borrelia burgdorferi with brain parenchyma elicits inflammatory mediators from glial cells as well as glial and neuronal apoptosis.
   Authors: Ramesh G, Borda JT, Dufour J, Kaushal D, Ramamoorthy R, Lackner AA, Philipp MT
   Am. J. Pathol., 2008;173(5):1415-27.
   Species: Primate - Macaca mulatta (Rhesus Macaque)
   Sample Type: Whole Tissue
   Application: IHC Frozen


9. Increased serum levels of the chemokine CXCL13 and up-regulation of its gene expression are distinctive features of HCV-related cryoglobulinemia and correlate with active cutaneous vasculitis.
   Authors: Sansonno D, Tucci FA, Troiani L, Lauletta G, Montrone M, Conteduca V, Sansonno L, Dammacco F
   Blood, 2008;112(5):1620-7.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


10. Developing neonatal rabbit appendix, a primary lymphoid organ, is seeded by immature blood-borne B cells: evidence for roles for CD62L/PNAd, CCR7/CCL21, alpha4beta1 and LFA-1.
    Authors: Sinha RK, Mage RG
    Dev. Comp. Immunol., 2004;28(7):829-41.
    Species: Rabbit
    Sample Type: Whole Tissue
    Application: IHC

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