抗IGF-Ⅰ抗体(Anti-IGF-Ⅰ, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse IGF-IGl33-Ala102Accession # Q8CAR0
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse IGF‑I in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human IGF-I is observed, and less than 2% cross-reactivity with recombinant mouse IGF-II is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse IGF-I (Catalog # 791-MG)
Immunohistochemistry	5-15 µg/mL	See below
Neutralization	Measured by its ability to neutralize IGF‑I-induced proliferation in the MCF‑7 human breast cancer cell line. Karey, K. P. et al. (1988) Cancer Research 48:4083. The Neutralization Dose (ND50) is typically 0.2-1.0 µg/mL in the presence of 15 ng/mL Recombinant Mouse IGF‑I.

Neutralization
	Cell Proliferation Induced by IGF‑I and Neutralization by Mouse IGF‑I Antibody. Recombinant Mouse IGF‑I (Catalog # 791-MG) stimulates proliferation in the MCF‑7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IGF‑I (15 ng/mL) is neutralized (green line) by increasing concen­trations of Goat Anti-Mouse IGF-I Antigen Affinity-purified Polyclonal Antibody (Catalog # AF791). The ND50 is typically 0.2-1.0 µg/mL.
Immunohistochemistry
	IGF‑I in Mouse Embryo. IGF‑I was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse IGF‑I Antigen Affinity-purified Polyclonal Antibody (Catalog # AF791) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to developing brain and muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

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Related Product & Information

Long Name	Insulin-like Growth Factor I
Entrez Gene IDs	3479 (Human); 16000 (Mouse); 24482 (Rat)
Background	IGF-I
background_content	Background: IGF-I Insulin-like growth factor I, also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Mouse IGF-I is synthesized as two precursor isoforms with alternate N- and C-terminal propeptides (1). These isoforms are differentially expressed by various tissues (1). The 7.6 kDa mature IGF-I is identical between isoforms and is generated by proteolytic removal of the N- and C-terminal regions. Mature mouse IGF-I shares 94% and 99% aa sequence identity with human and rat IGF-I, respectively (2), and exhibits cross-species activity. It shares 60% aa sequence identity with mature mouse IGF‑II. Circulating IGF-I is produced by hepatocytes, while local IGF-I is produced by many other tissues in which it has paracrine effects (1). IGF-I induces the proliferation, migration, and differentiation of a wide variety of cell types during development and postnatally (3). IGF-I regulates glucose and fatty acid metabolism, steroid hormone activity, and cartilage and bone metabolism (4-7). It plays an important role in muscle regeneration and tumor progression (1, 8). IGF-I binds IGF-I R, IGF-II R, and the insulin receptor, although its effects are mediated primarily by IGF-I R (9). IGF-I association with IGF binding proteins increases its plasma half-life and modulates its interactions with receptors (10).

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Citations

1. AHNAK Loss in Mice Promotes Type II Pneumocyte Hyperplasia and Lung Tumor Development
   Authors: JW Park, IY Kim, JW Choi, HJ Lim, JH Shin, YN Kim, SH Lee, Y Son, M Sohn, JK Woo, JH Jeong, C Lee, YS Bae, JK Seong
   Mol. Cancer Res., 2018;0(0):.
   Species: Mouse
   Sample Type: Tissue Micorarray
   Application: IHC


2. Systemic IGF-1 gene delivery by rAAV9 improves spontaneous autoimmune peripheral polyneuropathy (SAPP)
   Authors: T Gao, N Bogdanova, S Ghauri, G Zhang, J Lin, K Sheikh
   Sci Rep, 2018;8(1):5408.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. Immunization with Bacillus Calmette-Gu�rin (BCG) alleviates neuroinflammation and cognitive deficits in APP/PS1 mice via the recruitment of inflammation-resolving monocytes to the brain
   Authors: Z Zuo, F Qi, J Yang, X Wang, Y Wu, Y Wen, Q Yuan, J Zou, K Guo, ZB Yao
   Neurobiol. Dis, 2017;101(0):27-39.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


4. Combined Effects of Androgen and Growth Hormone on Osteoblast Marker Expression in Mouse C2C12 and MC3T3-E1 Cells Induced by Bone Morphogenetic Protein
   Authors: K Kimura, T Terasaka, N Iwata, T Katsuyama, M Komatsubar, R Nagao, K Inagaki, F Otsuka
   J Clin Med, 2017;6(1):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


5. Endothelial Cords Promote Tumor Initial Growth prior to Vascular Function through a Paracrine Mechanism
   Authors: C Zhao, W Zhang, Y Zhao, Y Yang, H Luo, G Ji, E Dong, H Deng, S Lin, Y Wei, H Yang
   Sci Rep, 2016;6(0):19404.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


6. Survival of neural stem cell grafts in the lesioned spinal cord is enhanced by a combination of treadmill locomotor training via insulin-like growth factor-1 signaling.
   Authors: Hwang D, Shin H, Kwon M, Choi J, Ryu B, Kim B
   J Neurosci, 2014;34(38):12788-800.
   Species: Rat
   Sample Type: In Vivo
   Application: In Vivo


7. Viral expression of insulin-like growth factor I E-peptides increases skeletal muscle mass but at the expense of strength.
   Authors: Brisson, Becky K, Spinazzola, Janelle, Park, SooHyun, Barton, Elisabet
   Am J Physiol Endocrinol Metab, 2014;306(8):E965-74.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


8. Attenuation of AD-like neuropathology by harnessing peripheral immune cells: local elevation of IL-10 and MMP-9.
   Authors: Koronyo-Hamaoui M, Ko MK, Koronyo Y, Azoulay D, Seksenyan A, Kunis G, Pham M, Bakhsheshian J, Rogeri P, Black KL, Farkas DL, Schwartz M
   J. Neurochem., 2009;111(6):1409-24.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


9. Role of IGF signaling in olfactory sensory map formation and axon guidance.
   Authors: Scolnick JA, Cui K, Duggan CD, Xuan S, Yuan XB, Efstratiadis A, Ngai J
   Neuron, 2008;57(6):847-57.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


10. Low-dose GH supplementation reduces the TLR2 and TNF-alpha expressions in visceral fat.
    Authors: Kubota Y, Unoki H, Bujo H, Rikihisa N, Udagawa A, Yoshimoto S, Ichinose M, Saito Y
    Biochem. Biophys. Res. Commun., 2008;368(1):81-7.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo

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