抗Myostatin抗体(Anti-Myostatin, Mouse, Goat-Poly antibody)

• 2025/12/01

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  [RSD]厳選 抗体＆リコンビナントタンパク質 30％OFFキャンペーン[～2026/02/27] NEW

  期間：2025/12/01 ～2026/02/27

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  R&D Systems, Inc.（RSD）

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse, Rat
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse GDF-8/MyostatinAsp268-Ser376Accession # O08689
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human, mouse, and rat GDF-8/Myostatin in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross‑reactivity is observed with recombinant human/mouse/rat GDF-11. In Western blots, no cross-reactivity with recombinant human BMP‑6 and recombinant mouse BMP-6 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below
Neutralization	Measured by its ability to neutralize GDF‑8/Myostatin-induced hemoglobin expression in the K562 human chronic myelogenous leukemia cell line. The Neutralization Dose (ND50) is typically 0.6-3 µg/mL in the presence of 30 ng/mL Recombinant Mouse GDF‑8/Myostatin.

Western Blot
	Detection of Recombinant Human, Mouse, and Rat GDF‑8/Myostatin by Western Blot. Western blot shows 25 ng of Recombinant Human/Mouse/Rat GDF‑8/Myostatin (Catalog # 788‑G8), Recombinant Human/Mouse/Rat GDF‑11/BMP‑11 (Catalog # 1958‑GD), Recombinant Human BMP‑6 (Catalog # 507‑BP), and Recombinant Mouse BMP‑6 (Catalog # 6325‑BM). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse/Rat GDF‑8/Myostatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF788) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for GDF‑8/Myostatin at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Immunohistochemistry
	GDF‑8/Myostatin in Mouse Embryo. GDF‑8/Myostatin was detected in immersion fixed frozen sections of mouse embryo (10 d.p.c., section through neural tube) using Goat Anti-Human/Mouse/Rat GDF‑8/Myostatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF788) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Neutralization
	Hemoglobin Expression Induced by GDF‑8/Myostatin and Neutralization by Mouse GDF‑8/Myostatin Antibody. Recombinant MouseGDF‑8/Myostatin (Catalog # 788-G8) increases hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the psuedoperoxidase assay. Hemoglobin expression elicited by Recombinant Mouse GDF‑8/Myostatin (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Mouse/Rat GDF‑8/Myostatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF788). The ND50 is typically 0.6-3 µg/mL.

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Related Product & Information

Long Name	Growth Differentiation Factor 8
Entrez Gene IDs	2660 (Human); 17700 (Mouse)
Background	GDF-8/Myostatin
background_content	Background: GDF-8/Myostatin Growth Differentiation Factor 8 (GDF-8), also known as myostatin, is a member of the TGF-beta superfamily that is expressed specifically in developing and adult skeletal muscle. GDF-8 cDNA encodes a 376 amino acid (aa) prepropeptide with a 24 aa residue signal peptide, a 223 aa residue amino-terminal propeptide, and a 109 aa residue carboxy-terminal mature protein. Mature GDF-8 contains the canonical 7-cysteine motif common to other TGF-beta superfamily members. Similar to the TGF-beta s, activins and BMP-11, GDF-8 also contains one extra pair of cysteine residues that is not found in other family members. The bioactive form of GDF-8 is a homodimer with an apparent molecular weight of approximately 25 kDa. GDF-8 is highly conserved across species. At the amino acid sequence level, mature human, mouse, rat and cow GDF-8 are 100% identical. Within the TGF-beta superfamily, GDF-8 is most closely related to BMP-11, a mammalian protein that acts as a dorsal mesoderm and neural inducer in Xenopus explants. The two proteins share 90% amino acid sequence identity within their mature chain. A targeted disruption of GDF-8 in mouse results in large mice with a widespread increase in skeletal muscle mass, indicating that GDF-8 is a negative regulator of skeletal muscle growth. A mutation in the bovine GDF-8 gene has been shown to be responsible for the double-muscled phenotype in cattle breeds such as Belgian Blue cattle that is characterized by an increase in muscle mass. GDF-8 has also been shown to inhibit preadipocyte differentiation to adipocytes. Mature GDF-8 binds to activin type II receptors and the binding is antagonized by the activin-binding protein, follistatin. R&D Systems recombinant GDF-8 preparations have been shown to act similarly to Activin A in both the Xenopus animal cap and the K562 assays.

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Citations

1. Compensatory anabolic signaling in the sarcopenia of experimental chronic arthritis
   Authors: RD Little, I Prieto-Pot, S Pérez-Baos, A Villalvill, P Gratal, F Cicuttini, R Largo, G Herrero-Be
   Sci Rep, 2017;7(1):6311.
   Species: Rabbit
   Sample Type: Tissue Homogenates
   Application: WB


2. Inhibition of GDF8 (Myostatin) accelerates bone regeneration in diabetes mellitus type 2
   Authors: C Wallner, H Jaurich, JM Wagner, M Becerikli, K Harati, M Dadras, M Lehnhardt, B Behr
   Sci Rep, 2017;7(1):9878.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


3. The Use of Platelet-Rich and Platelet-Poor Plasma to Enhance Differentiation of Skeletal Myoblasts: Implications for the Use of Autologous Blood Products for Muscle Regeneration
   Authors: O Miroshnych, WT Chang, JL Dragoo
   Am J Sports Med, 2017;45(4):945-953.
   Species: Human
   Sample Type: Protein
   Application: WB


4. Myostatin signaling is up-regulated in female patients with advanced heart failure
   Authors: J Ishida, M Konishi, M Saitoh, M Anker, SD Anker, J Springer
   Int. J. Cardiol., 2017;0(0):.
   Species: Human
   Sample Type: Tissue Homogenates
   Application: WB


5. A single heterochronic blood exchange reveals rapid inhibition of multiple tissues by old blood
   Nat Commun, 2016;7(0):13363.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Fresh


6. High concentrations of HGF inhibit skeletal muscle satellite cell proliferation in vitro by inducing expression of myostatin: a possible mechanism for reestablishing satellite cell quiescence in vivo.
   Authors: Yamada M, Tatsumi R, Yamanouchi K, Hosoyama T, Shiratsuchi S, Sato A, Mizunoya W, Ikeuchi Y, Furuse M, Allen RE
   Am. J. Physiol., Cell Physiol., 2010;298(3):C465-76.
   Species: Rat
   Sample Type: Whole Cells
   Application: ICC


7. Increased secretion and expression of myostatin in skeletal muscle from extremely obese women.
   Authors: Hittel DS, Berggren JR, Shearer J, Boyle K, Houmard JA
   Diabetes, 2009;58(1):30-8.
   Species: Human
   Sample Type: Tissue Homogenates
   Application: WB

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