抗E-Cadherin抗体(Anti-E-Cadherin, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse E-CadherinAsp157-Val709Accession # P09803
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse E-Cadherin in direct ELISAs and Western blots. In direct ELISAs, approximately 75% cross-reactivity with recombinant ratE-Cadherin is observed and less than 4% cross-reactivity with rhK-Cadherin, rhCadherin-8, rhCadherin-17, and recombinant mouse P-Cadherin is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.5 µg/mL	See below
Simple Western	5 µg/mL	See below
Flow Cytometry	0.25 µg/106 cells	D3 mouse embryonic stem cell line
Immunohistochemistry	5-15 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	5-15 µg/mL	See below

Western Blot
	Detection of Human and MouseE‑Cadherin by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, P19 mouse embryonal carcinoma cell line, and 4T1 mouse breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for E‑Cadherin at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
	E‑Cadherin in D3 Mouse Embryonic Stem Cell Line. E‑Cadherin was detected in immersion fixed D3 mouse embryonic stem cell line using Goat Anti-Human/MouseE‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Immunohistochemistry
	E‑Cadherin in Mouse Skin. E‑Cadherin was detected in perfusion fixed frozen sections of mouse skin using Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes in keratinocytes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Immunocytochemistry
	E‑Cadherin in Mouse Intestinal Organoids. E‑Cadherin was detected in immersion fixed mouse intestinal organoids using Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL001) and counterstained with DAPI (blue). Magnification shown at 100X (upper panel) and 40X (lower panel). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
	E‑Cadherin in Mouse Intestinal Organoids. E‑Cadherin was detected in immersion fixed mouse intestinal organoids using Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
	E‑Cadherin in Mouse Spinal Cord. E‑Cadherin was detected in perfusion fixed frozen sections of mouse spinal cord using Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to dorsal horn. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Simple Western
	Detection of Human and Mouse E‑Cadherin by Simple WesternTM. Simple Western lane view shows lysates of 4T1 mouse breast cancer cell line, P19 mouse embryonal carcinoma cell line, A431 human epithelial carcinoma cell line, and MCF‑7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for E‑Cadherin at approximately 128 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Related Product & Information

Background

E-Cadherin

background_content

Background: E-Cadherin Epithelial (E)‑Cadherin (ECAD), also known as cell-CAM120/80 in the human, uvomorulin in the mouse, Arc-1 in the dog, and L-CAM in the chicken, is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins, which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. E-Cadherin may also play a role in tumor development, as loss of E-Cadherin has been associated with tumor invasiveness. E-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium‑dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. E‑Cadherin contains five extracellular calcium-binding domains of approximately 110 amino acids each.

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Citations

1. Annexin A2 mediates secretion of collagen VI, pulmonary elasticity and apoptosis of bronchial epithelial cells.
   Authors: Dassah M, Almeida D, Hahn R, Bonaldo P, Worgall S, Hajjar K
   J Cell Sci, 2104;127(0):828-44.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: Neut


2. Desmosomal cadherin association with Tctex-1 and cortactin-Arp2/3 drives perijunctional actin polymerization to promote keratinocyte delamination
   Authors: O Nekrasova, RM Harmon, JA Broussard, JL Koetsier, LM Godsel, GN Fitz, ML Gardel, KJ Green
   Nat Commun, 2018;9(1):1053.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


3. Multicolor quantitative confocal imaging cytometry
   Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
   Nat. Methods, 2018;15(1):39-46.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


4. NANOS2 acts as an intrinsic regulator of gonocytes-to-spermatogonia transition in the murine testes
   Authors: HP Pui, Y Saga
   Mech. Dev., 2018;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


5. Distinct Requirements for FGFR1 and FGFR2 in Primitive Endoderm Development and Exit from Pluripotency
   Authors: A Molotkov, P Mazot, JR Brewer, RM Cinalli, P Soriano
   Dev. Cell, 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


6. Combined mutation of Apc, Kras and Tgfbr2 effectively drives metastasis of intestinal cancer
   Authors: E Sakai, M Nakayama, H Oshima, Y Kouyama, A Niida, S Fujii, A Ochiai, KI Nakayama, K Mimori, Y Suzuki, CP Hong, CY Ock, SJ Kim, M Oshima
   Cancer Res., 2017;0(0):.
   


7. Novel role for IL-22 in protection during chronic Mycobacterium tuberculosis HN878 infection
   Authors: P Treerat, O Prince, A Cruz-Lagun, M Muñoz-Torr, MA Salazar-Le, M Selman, B Fallert-Ju, TA Reinhardt, JF Alcorn, D Kaushal, J Zuñiga, J Rangel-Mor, JK Kolls, SA Khader
   Mucosal Immunol, 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


8. Maintenance of Taste Organs Is Strictly Dependent on Epithelial Hedgehog/GLI Signaling
   PLoS Genet, 2016;12(11):e1006442.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


9. A mucosal imprint left by prior Escherichia coli bladder infection sensitizes to recurrent disease
   Nat Microbiol, 2016;2(0):16196.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC - Paraffin embedded


10. Dazl is a target RNA suppressed by mammalian NANOS2 in sexually differentiating male germ cells
    Authors: Y Kato, T Katsuki, H Kokubo, A Masuda, Y Saga
    Nat Commun, 2016;7(0):11272.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - PFA fixed

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