抗Noggin抗体(Anti-Noggin, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse NogginLeu20-Cys232Accession # P97466
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse Noggin in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human Noggin is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse Noggin (Catalog # 1967-NG)
Immunohistochemistry	5-15 µg/mL	See below
Immunocytochemistry	5-15 µg/mL	See below

Immunohistochemistry
	Noggin in Embryonic Mouse Cardiac Tissue. Noggin was detected in immersion fixed frozen sections of embryonic mouse cardiac tissue (11 d.p.c.) using 15 µg/mL Goat Anti-Mouse Noggin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF719) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Immunocytochemistry
	Noggin in PC‑3 Human Cell Line. Noggin was detected in immersion fixed PC‑3 human prostate cancer cell line using Goat Anti-Mouse Noggin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF719) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Related Product & Information

Background

Noggin

background_content

Background: Noggin Noggin was originally cloned based on its dorsalizing activity in Xenopus embryos. Mammalian Noggins were subsequently identified and cloned from human, mouse and rat cDNA libraries. Mouse Noggin cDNA encodes a 232 amino acid (aa) residue precursor protein with 19 aa residue putative signal peptide that is cleaved to generate the 213 aa residue mature protein which is secreted as a homodimeric glycoprotein. Noggin is a highly conserved molecule. Mature mouse Noggin shares 99% and 83% aa sequence identity with human and Xenopus Noggin, respectively. Noggin has a complex pattern of expression during embryogenesis. In the adult, Noggin is expressed in the central nervous system and in several adult peripheral tissues such as lung, skeletal muscle and skin. Noggin has been shown to be a high-affinity BMP (bone morphogenetic protein) binding protein that antagonizes BMP bioactivities.

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Citations

1. Myo/Nog cells: targets for preventing the accumulation of skeletal muscle-like cells in the human lens.
   Authors: Gerhart J, Greenbaum M, Scheinfeld V, Fitzgerald P, Crawford M, Bravo-Nuevo A, Pitts M, George-Weinstein M
   PLoS ONE, 2014;9(4):e95262.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


2. Joint TGF-beta type II receptor-expressing cells: ontogeny and characterization as joint progenitors.
   Authors: Li T, Longobardi L, Myers T, Temple J, Chandler R, Ozkan H, Contaldo C, Spagnoli A
   Stem Cells Dev, 2013;22(9):1342-59.
   


3. BMP signalling permits population expansion by preventing premature myogenic differentiation in muscle satellite cells.
   Authors: Ono Y, Calhabeu F, Morgan JE
   Cell Death Differ., 2011;18(2):222-34.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


4. Noggin producing, MyoD-positive cells are crucial for eye development.
   Authors: Gerhart J, Pfautz J, Neely C, Elder J, DuPrey K, Menko AS, Knudsen K, George-Weinstein M
   Dev. Biol., 2009;336(1):30-41.
   Species: Chicken
   Sample Type: Whole Tissue
   Application: IHC


5. MyoD-positive epiblast cells regulate skeletal muscle differentiation in the embryo.
   Authors: Gerhart J, Elder J, Neely C, Schure J, Kvist T, Knudsen K, George-Weinstein M
   J. Cell Biol., 2006;175(2):283-92.
   Species: Chicken
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded

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