抗IL-17B抗体(Anti-IL-17B, Goat-Poly antibody)
掲載日情報：2021/01/28 現在Webページ番号：27232
IL-17Bに対する抗体(Anti-IL-17B, Goat-Poly )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 ：2024年05月19日 00時00分現在]
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Anti-IL-17B, Goat-Poly |
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Anti-Mouse IL-17B Affinity Purified PAb |
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[在庫・価格 ：2024年05月19日 00時00分現在]
Anti-IL-17B, Goat-Poly
文献数： 1
- 商品コード：AF1709
- メーカー：RSD
- 包装：100μg
- 価格：¥100,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
マッチドペア：Mouse IL-17B サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAF1709，スタンダードとして#1709-ML-025を用いる。 別名：Cytokine Zcyto7 Genbank No： 27190 Protein Accession No： Q9QXT6 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | Mouse | 免疫動物 | Goat | ||||
| 交差性 | Mouse | 適用 | Blocking,ELISA,FCM,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | |||||||
| 関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Mouse IL-17B Affinity Purified PAb
文献数： 0
- 商品コード：AF1709-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
※受注発注品。形状：溶液または凍結乾燥 別名：Cytokine Zcyto7 Genbank No： 27190 Protein Accession No： Q9QXT6 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | 法規備考 | ||||||
| 抗原種 | 免疫動物 | Goat | |||||
| 交差性 | Mouse | 適用 | Blocking,ELISA,FCM,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | |||||||
| 関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Mouse |
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| Label | Unconjugated |
| Immunogen | E. coli-derived recombinant mouse IL-17BHis21-Phe180Accession # Q9QXT6 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects mouse IL‑17B in ELISAs and Western blots. In sandwich immunoassays, approximately 40% cross-reactivity with recombinant human IL-17B is observed and less than 0.3% cross-reactivity with recombinant mouse (rm) IL‑17, rmIL-17C, rmIL-17D, rmIL-17E, andrmIL‑17F is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Mouse IL‑17B (Catalog # 1709-ML) |
| Blockade of Receptor-ligand Interaction | In a functional ELISA, 0.4 - 2 µg/mL of this antibody will block 50% of the binding of 10 μg/mL of biotinylated Recombinant Mouse IL-17 beta R Fc Chimera to immobilized Recombinant Mouse IL-17B (Catalog # 1709-ML) coated at 2 µg/mL (100 µL/well). At 30 μg/mL, this antibody will block >90% of the binding. | |
| CyTOF-ready | Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. | |
| Intracellular Staining by Flow Cytometry | 2.5 µg/106 cells | See below |
| Mouse IL-17B Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 0.2-0.8 µg/mL | Mouse IL‑17B Antibody (Catalog #AF1709 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Mouse IL‑17B Biotinylated Antibody (Catalog #BAF1709 ) |
| ELISA Standard | Recombinant Mouse IL-17B Protein (Catalog #1709-ML ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Intracellular Staining by Flow Cytometry |
![Intracellular Staining by Flow Cytometry IL-17B Antibody [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/IL-17B_AF1709_Flow_Cytometry_8161.jpg) click image to view larger | Detection of IL‑17B in M‑NFS‑60 Mouse Cell Line by Flow Cytometry. M‑NFS‑60 mouse myelogenous leukemia lymphoblast cell line was stained with Goat Anti-Mouse IL‑17B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1709, filled histogram) or isotype control antibody (Catalog # AB‑108‑C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: IL-17B | The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17, IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cystine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus (1, 2). With the exception of IL-17B, which exists as a non‑covalently linked dimer, all IL-17 family members are disulfide-linked dimers (3). IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2). Two receptors (IL-17 R and IL-17B R), which are activated by IL-17 family members have been identified. In addition, at least three additional orphan type I transmembrane receptors with homology to IL-17 R, including IL-17 RL (IL-17 RC), IL-17 RD, and |
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References
| Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1. | |
| Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155. | |
| Hymowitz, S.G. et al. (2001) EMBO J. 20:5332. | |
| Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309. | |
| Shi, Y. et al. (2000) J. Biol. Chem. 275:19167. | |
| Li, H. et al. (2000) Proc. Natl. Acad. Sci. USA 97:773. | |
| Long Name: | Interleukin 17B |
| Entrez Gene IDs: | 27190 (Human); 56069 (Mouse) |
| Alternate Names: | Cytokine Zcyto7; cytokine-like protein ZCYTO7; IL17B; IL-17B; IL-17Binterleukin-17 beta; IL20; IL-20interleukin-17B; interleukin 17B; interleukin 20; Interleukin-20; MGC138900; MGC138901; neuronal interleukin-17 related factor; Neuronal interleukin-17-related factor; NIRF; ZCYTO7interleukin-20 |
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Related Research Areas
| Helper T (Th) Cells | ||
| IL-17 Family | ||
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| Intracellular Staining by Flow Cytometry | |
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 | Detection of IL‑17B in M‑NFS‑60 Mouse Cell Line by Flow Cytometry. M‑NFS‑60 mouse myelogenous leukemia lymphoblast cell line was stained with Goat Anti-Mouse IL‑17B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1709, filled histogram) or isotype control antibody (Catalog # AB‑108‑C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. |
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Related Product & Information
| Long Name | Interleukin 17B |
|---|---|
| Entrez Gene IDs | 27190 (Human); 56069 (Mouse) |
| Background | IL-17B |
| background_content | Background: IL-17B The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17, IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cystine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus (1, 2). With the exception of IL-17B, which exists as a non‑covalently linked dimer, all IL-17 family members are disulfide-linked dimers (3). IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2). Two receptors (IL-17 R and IL-17B R), which are activated by IL-17 family members have been identified. In addition, at least three additional orphan type I transmembrane receptors with homology to IL-17 R, including IL-17 RL (IL-17 RC), IL-17 RD, andIL‑17 RE, have also been reported (1‑4). Mouse IL-17B cDNA encodes a 180 amino acid residue (aa) protein with a putative 20 aa signal peptide (5). Mouse and human IL-17B share 88% aa sequence identity. Among IL-17 family members, mouse IL-17B is most closely related to mouse IL-17D, sharing 33% aa sequence homology. IL-17B is expressed highly in spinal cord, and at lower levels in brain, kidney, lung, small intestine, prostate, testes, pancreas, adrenal gland and trachea (5‑7). Expression of IL-17B has also been detected in chondrocytes in articular cartilage (2). IL-17B binds the IL-17B receptor but not IL-17 R and exhibits bioactivities distinct from those of IL-17 (5, 6). |
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Citations
- IL-17B and IL-17C are associated with TNF-alpha production and contribute to the exacerbation of inflammatory arthritis.
Authors: Yamaguchi Y, Fujio K, Shoda H, Okamoto A, Tsuno NH, Takahashi K, Yamamoto K
J. Immunol., 2007;179(10):7128-36.
Species: Mouse
Sample Type: In Vivo
Application: In vivo
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