抗Podocalyxin抗体(Anti-Podocalyxin, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human PodocalyxinSer23-Arg427Accession # AAB61574
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human Podocalyxin in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse Podocalyxin and recombinant human Endoglycan.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Flow Cytometry	0.25 µg/106 cells	BG01V human embryonic stem cells
Immunohistochemistry	5-15 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Knockout Validated	Podocalyxin/PODXL is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in Podocalyxin/PODXL knockout HeLa cell line.

Western Blot
	Detection of Human Podocalyxin by Western Blot. Western blot shows lysates of human kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1658) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Podocalyxin at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
	Podocalyxin in Human Kidney. Podocalyxin was detected in immersion fixed paraffin-embedded sections of human kidney using 5 µg/mL Goat Anti-Human Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1658) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to podocytes in glomeruli. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Knockout Validated
	Western Blot Shows Human Podocalyxin Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Podocalyxin/PODXL knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1658) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Podocalyxin at approximately 160 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Related Product & Information

Background

Podocalyxin

background_content

Background: Podocalyxin Podocalyxin, also known as Podocalyxin-like protein-1 (PCLP1 or PODXL), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology. Podocalyxin is a major sialoprotein in the podocytes of the kidney glomerulus and is also expressed by both endothelium and multipotent hematopoietic progenitors. It has been identified as a novel cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells (1, 2).

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Citations

1. Glycome analysis of extracellular vesicles derived from human induced pluripotent stem cells using lectin microarray
   Authors: S Saito, K Hiemori, K Kiyoi, H Tateno
   Sci Rep, 2018;8(1):3997.
   Species: Human
   Sample Type: Protein
   Application: WB


2. Overexpression of preeclampsia induced microRNA-26a-5p leads to proteinuria in zebrafish
   Authors: J Müller-Dei, P Schröder, L Beverly-St, R Hiss, J Fiedler, J Nyström, T Thum, H Haller, M Schiffer
   Sci Rep, 2018;8(1):3621.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


3. Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells
   Nat Protoc, 2017;12(1):195-207.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Not Specified


4. Lymphatic endothelial progenitors originate from plastic myeloid cells activated by toll-like receptor-4
   Authors: LD Volk-Drape, KL Hall, AC Wilber, S Ran
   PLoS ONE, 2017;12(6):e0179257.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


5. Loss of podocalyxin causes a novel syndromic type of congenital nephrotic syndrome
   Authors: HG Kang, M Lee, KB Lee, M Hughes, BS Kwon, S Lee, KM McNagny, YH Ahn, JM Ko, IS Ha, M Choi, HI Cheong
   Exp. Mol. Med., 2017;49(12):e414.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


6. ?-Catenin Is Required for Endothelial Cyp1b1 Regulation Influencing Metabolic Barrier Function
   J Neurosci, 2016;36(34):8921-35.
   Species: Mouse
   Sample Type: Whole Cells
   Application: IHC Fresh


7. Reversible commitment to differentiation by human multipotent stromal cells in single-cell-derived colonies.
   Authors: Ylostalo J, Bazhanov N, Prockop DJ
   Exp. Hematol., 2008;36(10):1390-402.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC

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