抗TROP-2抗体(Anti-TROP-2, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human TROP-2Thr88-Thr274Accession # P09758
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human TROP-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) MCAM, rhNCAM‑L1, and rhBCAM is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Flow Cytometry	2.5 µg/106 cells	See below
Immunohistochemistry	5-15 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Western Blot
	Detection of Human TROP‑2 by Western Blot. Western blot shows lysates of NCI‑N87 human gastric carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TROP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF650) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TROP‑2 at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Flow Cytometry
	Detection of TROP‑2 in PC‑3 Human Cell Line by Flow Cytometry. PC‑3 human prostate cancer cell line was stained with Goat Anti-Human TROP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF650, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
Immunohistochemistry
	TROP‑2 in Human Brain. TROP‑2 was detected in immersion fixed paraffin-embedded sections of human brain (frontal cortex) using Goat Anti-Human TROP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF650) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

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Related Product & Information

Background

TROP-2

background_content

Background: TROP-2 Human TROP-2, also called tumor associated calcium signal transducer 2 (TACSTD2), GA733-1, gp50 and T16, is a type I cell surface glycoprotein that is highly expressed on human carcinomas. It was originally identified as an antigen present on human gastrointestinal tumors and is the second of two members of this family. The other family member is GA733-2, also called EpCAM, TROP-1, 17-1A, gp40 and KSA. The TROP-2 gene is unique in that it contains no introns. A study of these two genes suggested that TROP-2 was the result of a retroposition of the EpCAM gene. TROP-2 and EpCAM share approximately 49% amino acid identity and approximately 67% similarity. Human and mouse TROP-2 share 87% similarity. The human TROP-2 protein consists of a putative 26 amino acid (aa) signal sequence, a 248 aa extracellular domain, a 23 aa transmembrane region and a 26 aa cytoplasmic domain. TROP-2 is capable of transducing an intracellular calcium signal and may play a role in tumor growth. It also has adhesive functions.

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Citations

1. Trop2 enhances invasion of thyroid cancer by inducing MMP2 through ERK and JNK pathways
   Authors: H Guan, Z Guo, W Liang, H Li, G Wei, L Xu, H Xiao, Y Li
   BMC Cancer, 2017;17(1):486.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


2. First-in-Human Trial of a Novel Anti-Trop-2 Antibody-SN-38 Conjugate, Sacituzumab Govitecan, for the Treatment of Diverse Metastatic Solid Tumors.
   Authors: Starodub A, Ocean A, Shah M, Guarino M, Picozzi V, Vahdat L, Thomas S, Govindan S, Maliakal P, Wegener W, Hamburger S, Sharkey R, Goldenberg D
   Clin Cancer Res, 2015;21(17):3870-8.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Not Specified


3. Trop-2 is a determinant of breast cancer survival.
   Authors: Ambrogi, Federico, Fornili, Marco, Boracchi, Patrizia, Trerotola, Marco, Relli, Valeria, Simeone, Pasquale, La Sorda, Rossana, Lattanzio, Rossano, Querzoli, Patrizia, Pedriali, Massimo, Piantelli, Mauro, Biganzoli, Elia, Alberti, Saverio
   PLoS ONE, 2014;9(5):e96993.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Expression of tumor-associated calcium signal transducer 2 in patients with salivary adenoid cystic carcinoma: Correlation with clinicopathological features and prognosis.
   Authors: Xia Y, Li B, Gao N, Xia H, Men Y, Liu Y, Liu Z, Chen Q, Li L
   Oncol Lett, 2014;8(4):1670-1674.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


5. Upregulation of Trop-2 quantitatively stimulates human cancer growth.
   Authors: Trerotola, M, Cantanelli, P, Guerra, E, Tripaldi, R, Aloisi, A L, Bonasera, V, Lattanzio, R, de Lange, R, Weidle, U H, Piantelli, M, Alberti, S
   Oncogene, 2013;32(2):222-33.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


6. Uterine and ovarian carcinosarcomas overexpressing Trop-2 are sensitive to hRS7, a humanized anti-Trop-2 antibody.
   Authors: Raji R, Guzzo F, Carrara L
   J. Exp. Clin. Cancer Res., 2011;30(0):106.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


7. Identification of TROP2 (TACSTD2), an EpCAM-like molecule, as a specific marker for TGF-beta1-dependent human epidermal Langerhans cells.
   Authors: Eisenwort G, Jurkin J, Yasmin N, Bauer T, Gesslbauer B, Strobl H
   J. Invest. Dermatol., 2011;131(10):2049-57.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


8. Tumor-associated calcium signal transducer 2 is required for the proper subcellular localization of claudin 1 and 7: implications in the pathogenesis of gelatinous drop-like corneal dystrophy.
   Authors: Nakatsukasa M, Kawasaki S, Yamasaki K
   Am. J. Pathol., 2010;177(3):1344-55.
   Species: Human
   Sample Type: Cell Lysates
   Application: IP


9. Identification of Trop-2 as an oncogene and an attractive therapeutic target in colon cancers.
   Authors: Wang J, Day R, Dong Y, Weintraub SJ, Michel L
   Mol. Cancer Ther., 2008;7(2):280-5.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut

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