抗E-Cadherin抗体(Anti-E-Cadherin, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human E-CadherinAsp155-Ile707Accession # P12830
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human E-Cadherin in direct ELISAs and Western blots.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.5 µg/mL	See below
Simple Western	5 µg/mL	See below
Flow Cytometry	0.25 µg/106 cells	See below
Immunohistochemistry	0.3-15 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	5-15 µg/mL	See below

Western Blot
	Detection of Human and MouseE‑Cadherin by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, P19 mouse embryonal carcinoma cell line, and 4T1 mouse breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for E‑Cadherin at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Flow Cytometry
	Detection of E‑Cadherin in MCF‑7 Human Cell Line by Flow Cytometry. MCF‑7 human breast cancer cell line was stained with Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.
Immunocytochemistry
	E‑Cadherin in Human Epidermoid Carcinoma Cells. E‑Cadherin was detected in immersion fixed human epidermoid carcinoma cells using 10 µg/mL Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
	E‑Cadherin and SOX2 in BG01V Human Stem Cells. E‑Cadherin and SOX2 were detected in BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) and 10 µg/mL Human/Mouse SOX2 Monoclonal Antibody (Catalog # MAB2018). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for E‑Cadherin using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL001) and for SOX2 using the NorthernLights 493-conjugated Anti-Mouse Secondary Antibody (red; Catalog # NL009). Cells were counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
	E‑Cadherin in Human Stomach. E‑Cadherin was detected in immersion fixed paraffin-embedded sections of human stomach using Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane and cytoplasm in gastric glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Simple Western
	Detection of Human and Mouse E‑Cadherin by Simple WesternTM. Simple Western lane view shows lysates of 4T1 mouse breast cancer cell line, P19 mouse embryonal carcinoma cell line, A431 human epithelial carcinoma cell line, and MCF‑7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for E‑Cadherin at approximately 128 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Related Product & Information

Background

E-Cadherin

background_content

Background: E-Cadherin Epithelial (E)‑Cadherin (ECAD), also known as cell-CAM120/80 in the human, uvomorulin in the mouse, Arc-1 in the dog, and L-CAM in the chicken, is a member of the Cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. E‑Cadherin may also play a role in tumor development, as loss of E‑Cadherin has been associated with tumor invasiveness. E‑Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium‑dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. E‑Cadherin contains five extracellular calcium‑binding domains of approximately 110 amino acids each.

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Citations

1. Placenta-specific protein 1 promotes cell proliferation and invasion in non-small cell lung cancer
   Authors: L Yang, TQ Zha, X He, L Chen, Q Zhu, WB Wu, FQ Nie, Q Wang, CS Zang, ML Zhang, J He, W Li, W Jiang, KH Lu
   Oncol. Rep., 2018;39(1):53-60.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


2. Wnt/?-catenin promotes gastric fundus specification in mice and humans
   Authors: KW McCracken, E Aihara, B Martin, CM Crawford, T Broda, J Treguier, X Zhang, JM Shannon, MH Montrose, JM Wells
   Nature, 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


3. Novel role for IL-22 in protection during chronic Mycobacterium tuberculosis HN878 infection
   Authors: P Treerat, O Prince, A Cruz-Lagun, M Muñoz-Torr, MA Salazar-Le, M Selman, B Fallert-Ju, TA Reinhardt, JF Alcorn, D Kaushal, J Zuñiga, J Rangel-Mor, JK Kolls, SA Khader
   Mucosal Immunol, 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


4. miR-151a induces partial EMT by regulating E-cadherin in NSCLC cells
   Authors: I Daugaard, KJ Sanders, A Idica, K Vittayaruk, M Hamdorf, JD Krog, R Chow, D Jury, LL Hansen, H Hager, P Lamy, CL Choi, D Agalliu, DG Zisoulis, IM Pedersen
   Oncogenesis, 2017;6(7):e366.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


5. Surface Topography Guides Morphology and Spatial Patterning of Induced Pluripotent Stem Cell Colonies
   Authors: G Abagnale, A Sechi, M Steger, Q Zhou, CC Kuo, G Aydin, C Schalla, G Müller-New, M Zenke, IG Costa, P van Rijn, A Gillner, W Wagner
   Stem Cell Reports, 2017;9(2):654-666.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


6. Directed Differentiation of Human Induced Pluripotent Stem Cells into Fallopian Tube Epithelium
   Authors: N Yucer, M Holzapfel, T Jenkins Vo, L Lenaeus, L Ornelas, A Laury, D Sareen, R Barrett, BY Karlan, CN Svendsen
   Sci Rep, 2017;7(1):10741.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


7. Canine spontaneous head and neck squamous cell carcinomas represent their human counterparts at the molecular level.
   Authors: Liu D, Xiong H, Ellis A, Northrup N, Dobbin K, Shin D, Zhao S
   PLoS Genet, 2015;11(6):e1005277.
   Species: Canine
   Sample Type: Whole Cells
   Application: IHC Paraffin-embedded


8. Molecular homology and difference between spontaneous canine mammary cancer and human breast cancer.
   Authors: Liu D, Xiong H, Ellis A, Northrup N, Rodriguez C, O'Regan R, Dalton S, Zhao S
   Cancer Res, 2014;74(18):5045-56.
   Species: Canine
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


9. Penton-dodecahedral particles trigger opening of intercellular junctions and facilitate viral spread during adenovirus serotype 3 infection of epithelial cells.
   Authors: Lu Z, Wang H, Zhang Y, Cao H, Li Z, Fender P, Lieber A
   PLoS Pathog, 2013;9(10):e1003718.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


10. Interleukin-15 plays a central role in human kidney physiology and cancer through the gamma c signaling pathway.
    Authors: Giron-Michel J, Azzi S, Khawam K, Mortier E, Caignard A, Devocelle A, Ferrini S, Croce M, Francois H, Lecru L, Charpentier B, Chouaib S, Azzarone B, Eid P
    PLoS ONE, 2012;7(2):e31624.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC

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