抗WNT-5a抗体(Anti-WNT-5a, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse, Rat
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse Wnt‑5a peptideGln254-Cys334Accession # P22725
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse and rat Wnt-5a in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) Wnt‑5b is observed and less than 2% cross-reactivity with rmWnt-1, rmWnt-3a, rmWnt-4, rmWnt-11, and rmWnt-16 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below

Immunohistochemistry
	Wnt‑5a in Mouse Embryonic Rib. Wnt‑5a was detected in immersion fixed paraffin-embedded sections of mouse embryonic rib using 15 µg/mL Mouse/Rat Wnt‑5a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF645) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Western Blot
	Detection of Mouse Wnt‑5a by Western Blot. Western blot shows lysates of mouse brain and lactating mammary tissue. PVDF membrane was probed with 1 µg/mL of Mouse/Rat Wnt‑5a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF645) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Wnt‑5a at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Immunohistochemistry
	Wnt‑5a in Mouse Embryo. Wnt‑5a was detected in immersion fixed frozen sections of mouse embryo using Mouse/Rat Wnt‑5a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF645) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

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Related Product & Information

Background

Wnt-5a

background_content

Background: Wnt-5a Wnt proteins are secreted glycoproteins that contain a conserved pattern of 23-24 cysteine residues. Wnts play critical roles in both carcinogenesis and embryonic development for a variety of organisms. Wnts bind to receptors of the Frizzled family, sometimes in conjunction with other membrane-associated proteins such as LRPs or proteoglycans. Downstream effects of Wnt signaling occur through different intracellular components, depending on which pathway is activated. Three pathways have been characterized: the canonical Wnt/ beta -catenin pathway, the Wnt/Ca2+ pathway, and the planar cell polarity (1, 2). Wnt-5a is part of the subgroup of Wnts that are not axis-inducing in Xenopus embryos and do not transform C57MG mammary epithelial cells. This subgroup is also implicated in the Wnt/Ca2+ pathway, playing roles in cell movements and cell adhesion (3). This non-canonical Wnt pathway can inhibit canonical Wnt/ beta -catenin signaling. In Wnt-5a deficient mouse embryos, beta -catenin accumulates in the limb bud suggesting that Wnt-5a normally promotes degradation of beta -catenin (4). Likewise, in Xenopus embryos Wnt-5a antagonizes the ability of the canonical Wnt subgroup to induce a secondary axis (5). Wnt-5a is implicated in various types of cancer and has complex roles. It acts as a tumor suppressor for mammary, B-cell, colon, and uroepithelial cancer cells but is up-regulated in melanomas, where expression levels correlate with severity of metastasis (3). Furthermore, aberrant Wnt-5a signaling results in other diseases such as rheumatoid arthritis (6). Like other developmental growth factors Wnt-5a has diverse roles in development. They are too numerous to enunciate here, as functions span from early anterior-posterior development and gastrulation movements to maintaining hematopoietic stem cell population, lung morphogenesis, and limb outgrowth. Mouse and human Wnt-5a share 97% amino acid identity.

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Citations

1. Ror family receptor tyrosine kinases regulate the maintenance of neural progenitor cells in the developing neocortex.
   J. Cell. Sci., 2102;125(0):2017-29.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


2. Reduced Cdc42 Activity Compromises Hematopoiesis-Supportive Function Of Fanconi Anemia Mesenchymal Stromal Cells
   Authors: J Xu, X Li, A Cole, Z Sherman, W Du
   Stem Cells, 2018;0(0):.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


3. Wnt5A/Ryk signaling critically affects barrier function in human vascular endothelial cells
   Authors: T Skaria, E Bachli, G Schoedon
   Cell Adh Migr, 2017;11(1):24-38.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


4. Wnt5a is essential for hippocampal dendritic maintenance and spatial learning and memory in adult mice
   Authors: CM Chen, LL Orefice, SL Chiu, TA LeGates, S Hattar, RL Huganir, H Zhao, B Xu, R Kuruvilla
   Proc. Natl. Acad. Sci. U.S.A, 2017;114(4):E619-E628.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


5. Butyrate and bioactive proteolytic form of Wnt-5a regulate colonic epithelial proliferation and spatial development
   Sci Rep, 2016;6(0):32094.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


6. Noncanonical WNT-5A signaling impairs endogenous lung repair in COPD
   Authors: Hoeke A Baarsma
   J. Exp. Med, 2016;0(0):.
   Species: Mouse
   Sample Type: In Vivo
   Application: Neut


7. IL-4 Causes Hyperpermeability of Vascular Endothelial Cells through Wnt5A Signaling
   PLoS ONE, 2016;11(5):e0156002.
   Species: Human
   Sample Type: Whole Cells
   Application: IHC - Not specified


8. Wnt-5a/Frizzled9 receptor signaling through the G?o/G?? complex regulates dendritic spine formation
   J Biol Chem, 2016;0(0):.
   Species: N/A
   Sample Type: Recombinant Protein
   Application: Bioassay


9. WINGLESS (WNT) signaling is a progesterone target for rat uterine stromal cell proliferation
   Authors: Bruce F Kimler
   J. Endocrinol., 2016;229(2):197-207.
   Species: Rat
   Sample Type: Whole Tissue
   Application: IHC-P


10. WNT5A induces release of exosomes containing pro-angiogenic and immunosuppressive factors from malignant melanoma cells.
    Authors: Ekstrom E, Bergenfelz C, von Bulow V, Serifler F, Carlemalm E, Jonsson G, Andersson T, Leandersson K
    Mol Cancer, 2014;13(0):88.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB

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