抗WNT-5a抗体(Anti-WNT-5a, Mouse, Goat-Poly antibody)

掲載日情報:2018/11/26 現在Webページ番号:27185

WNT-5aに対する抗体(Anti-WNT-5a, Mouse, Goat-Poly )です。
本製品は研究用です。研究用以外には使用できません。

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[在庫・価格 :2024年06月15日 18時35分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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納期 文献数
Anti-WNT-5a, Mouse, Goat-Poly
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 57
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説明文
別名:hWNT5A
Genbank No: 7474
Protein Accession No: P22725
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Mouse 免疫動物 Goat クラス IgG 標識 Unlabeled
交差性 Mouse 適用 IHC,Western Blot
クロナリティ Polyclonal フォーマット 性状 Antigen Affinity Purified 吸収処理
掲載カタログ

製品記事 免疫染色システム ImmPRESS® Reagent Anti-Goat IgG
R&D Systems 社 Wnt-5a関連製品
Wnt関連製品
関連記事
Anti-Mouse/Rat Wnt-5a Affinity Purified Polyclonal Ab
2~3週間 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 6
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  • メーカーサイト
  • お問い合わせ
説明文
※受注発注品。形状:溶液または凍結乾燥
別名:hWNT5A
Genbank No: 7474
Protein Accession No: P22725
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 免疫動物 Goat クラス IgG 標識 Unlabeled
交差性 Mouse 適用 IHC,Western Blot
クロナリティ Polyclonal フォーマット 性状 Antigen Affinity Purified 吸収処理
掲載カタログ

製品記事 免疫染色システム ImmPRESS® Reagent Anti-Goat IgG
Wnt関連製品
関連記事

[在庫・価格 :2024年06月15日 18時35分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-WNT-5a, Mouse, Goat-Poly

文献数: 57

説明文 別名:hWNT5A
Genbank No: 7474
Protein Accession No: P22725
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Mouse 免疫動物 Goat
交差性 Mouse 適用 IHC,Western Blot
標識 Unlabeled 性状 Antigen Affinity Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 免疫染色システム ImmPRESS® Reagent Anti-Goat IgG
R&D Systems 社 Wnt-5a関連製品
Wnt関連製品
関連記事

Anti-Mouse/Rat Wnt-5a Affinity Purified Polyclonal Ab

文献数: 6

説明文 ※受注発注品。形状:溶液または凍結乾燥
別名:hWNT5A
Genbank No: 7474
Protein Accession No: P22725
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 免疫動物 Goat
交差性 Mouse 適用 IHC,Western Blot
標識 Unlabeled 性状 Antigen Affinity Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 免疫染色システム ImmPRESS® Reagent Anti-Goat IgG
Wnt関連製品
関連記事



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Product Details

Species ReactivityMouse, Rat
LabelUnconjugated
ImmunogenE. coli-derived recombinant mouse Wnt‑5a peptideGln254-Cys334Accession # P22725
SourcePolyclonal Goat IgG
PurificationAntigen Affinity-purified
SpecificityDetects mouse and rat Wnt-5a in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) Wnt‑5b is observed and less than 2% cross-reactivity with rmWnt-1, rmWnt-3a, rmWnt-4, rmWnt-11, and rmWnt-16 is observed.


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Applications and Data

 Recommended
Concentration
Sample
Western Blot1 µg/mLSee below
Immunohistochemistry5-15 µg/mLSee below


Immunohistochemistry
Wnt‑5a in Mouse Embryonic Rib.
Wnt‑5a was detected in immersion fixed paraffin-embedded sections of mouse embryonic rib using 15 µg/mL Mouse/Rat Wnt‑5a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF645) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Western Blot
Detection of Mouse Wnt‑5a by Western Blot.
Western blot shows lysates of mouse brain and lactating mammary tissue. PVDF membrane was probed with 1 µg/mL of Mouse/Rat Wnt‑5a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF645) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Wnt‑5a at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Immunohistochemistry
Wnt‑5a in Mouse Embryo.
Wnt‑5a was detected in immersion fixed frozen sections of mouse embryo using Mouse/Rat Wnt‑5a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF645) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.


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Related Product & Information

BackgroundWnt-5a
background_contentBackground:
Wnt-5a
Wnt proteins are secreted glycoproteins that contain a conserved pattern of 23-24 cysteine residues. Wnts play critical roles in both carcinogenesis and embryonic development for a variety of organisms. Wnts bind to receptors of the Frizzled family, sometimes in conjunction with other membrane-associated proteins such as LRPs or proteoglycans. Downstream effects of Wnt signaling occur through different intracellular components, depending on which pathway is activated. Three pathways have been characterized: the canonical Wnt/ beta -catenin pathway, the Wnt/Ca2+ pathway, and the planar cell polarity (1, 2). Wnt-5a is part of the subgroup of Wnts that are not axis-inducing in Xenopus embryos and do not transform C57MG mammary epithelial cells. This subgroup is also implicated in the Wnt/Ca2+ pathway, playing roles in cell movements and cell adhesion (3). This non-canonical Wnt pathway can inhibit canonical Wnt/ beta -catenin signaling. In Wnt-5a deficient mouse embryos, beta -catenin accumulates in the limb bud suggesting that Wnt-5a normally promotes degradation of beta -catenin (4). Likewise, in Xenopus embryos Wnt-5a antagonizes the ability of the canonical Wnt subgroup to induce a secondary axis (5). Wnt-5a is implicated in various types of cancer and has complex roles. It acts as a tumor suppressor for mammary, B-cell, colon, and uroepithelial cancer cells but is up-regulated in melanomas, where expression levels correlate with severity of metastasis (3). Furthermore, aberrant Wnt-5a signaling results in other diseases such as rheumatoid arthritis (6). Like other developmental growth factors Wnt-5a has diverse roles in development. They are too numerous to enunciate here, as functions span from early anterior-posterior development and gastrulation movements to maintaining hematopoietic stem cell population, lung morphogenesis, and limb outgrowth. Mouse and human Wnt-5a share 97% amino acid identity.


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Citations

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
  1. Ror family receptor tyrosine kinases regulate the maintenance of neural progenitor cells in the developing neocortex.
    J. Cell. Sci., 2102;125(0):2017-29.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB

  2. Reduced Cdc42 Activity Compromises Hematopoiesis-Supportive Function Of Fanconi Anemia Mesenchymal Stromal Cells
    Authors: J Xu, X Li, A Cole, Z Sherman, W Du
    Stem Cells, 2018;0(0):.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB

  3. Wnt5A/Ryk signaling critically affects barrier function in human vascular endothelial cells
    Authors: T Skaria, E Bachli, G Schoedon
    Cell Adh Migr, 2017;11(1):24-38.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC

  4. Wnt5a is essential for hippocampal dendritic maintenance and spatial learning and memory in adult mice
    Authors: CM Chen, LL Orefice, SL Chiu, TA LeGates, S Hattar, RL Huganir, H Zhao, B Xu, R Kuruvilla
    Proc. Natl. Acad. Sci. U.S.A, 2017;114(4):E619-E628.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB

  5. Butyrate and bioactive proteolytic form of Wnt-5a regulate colonic epithelial proliferation and spatial development
    Sci Rep, 2016;6(0):32094.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut

  6. Noncanonical WNT-5A signaling impairs endogenous lung repair in COPD
    Authors: Hoeke A Baarsma
    J. Exp. Med, 2016;0(0):.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut

  7. IL-4 Causes Hyperpermeability of Vascular Endothelial Cells through Wnt5A Signaling
    PLoS ONE, 2016;11(5):e0156002.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC - Not specified

  8. Wnt-5a/Frizzled9 receptor signaling through the G?o/G?? complex regulates dendritic spine formation
    J Biol Chem, 2016;0(0):.
    Species: N/A
    Sample Type: Recombinant Protein
    Application: Bioassay

  9. WINGLESS (WNT) signaling is a progesterone target for rat uterine stromal cell proliferation
    Authors: Bruce F Kimler
    J. Endocrinol., 2016;229(2):197-207.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC-P

  10. WNT5A induces release of exosomes containing pro-angiogenic and immunosuppressive factors from malignant melanoma cells.
    Authors: Ekstrom E, Bergenfelz C, von Bulow V, Serifler F, Carlemalm E, Jonsson G, Andersson T, Leandersson K
    Mol Cancer, 2014;13(0):88.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB



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