抗Flk-1抗体(Anti-Flk-1, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse VEGF R2Ala20-Glu762Accession # P35918
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse VEGF R2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human VEGF R2 is observed and less than 2% cross-reactivity with recombinant mouse (rm) VEGF R1 and rmVEGF R3 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse VEGF R2/KDR/Flk‑1 Fc Chimera (Catalog # 443-KD)
Flow Cytometry	2.5 µg/106 cells	bEnd.3 mouse endothelioma cell line
Immunohistochemistry	5-15 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Neutralization	Measured by its ability to neutralize VEGF R2/KDR/Flk‑1-mediated inhibition of proliferation in HUVEC human umbilical vein endothelial cells. The Neutralization Dose (ND50) is typically 0.1-0.3 µg/mL in the presence of 50 ng/mL Recombinant Mouse VEGF R2/KDR/Flk‑1 Fc Chimera and 5 ng/mL Recombinant Mouse VEGF164.

Immunohistochemistry
	VEGF R2/KDR/Flk‑1 in Mouse Embryo. VEGF R2/KDR/Flk‑1 was detected in immersion fixed frozen sections of mouse embryo (14 d.p.c.) using 15 µg/mL Goat Anti-Mouse VEGF R2/KDR/Flk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF644) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to mesenchymal cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Immunohistochemistry
	VEGF R2 in Mouse Kidney Tissue. VEGFR2 was detected in acetone fixed cryosections of mouse kidney tissue using Goat Anti-Mouse VEGF R2/KDR/Flk-1 Polyclonal Antibody (Catalog # AF644) for 50 minutes at room temperature. Tissues were stained with rabbit anti-goat secondaryantibody and HRP polymer-conjugated anti-rabbit IgG followed by AEC+Substrate Chromogen (red) followed by counterstaining with hematoxylin (blue). Experiments were carried out and the image was provided by Dr. Grietje Molema, University Medical Center Groningen, The Netherlands.
Neutralization
	VEGF R2/KDR/Flk‑1 Inhibition of VEGF-dependent Cell Proliferation and Neutralization by Mouse VEGF R2/KDR/Flk‑1 Antibody. Recombinant Mouse VEGF R2/KDR/Flk‑1 Fc Chimera (Catalog # 443-KD) inhibits Recombinant Mouse VEGF164 (Catalog # 493-MV) induced proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse VEGF164 (5 ng/mL) activity elicited by Recombinant Mouse VEGF R2/KDR/Flk‑1 Fc Chimera (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse VEGF R2/KDR/Flk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF644). The ND50 is typically 0.1‑0.3 µg/mL.

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Related Product & Information

Long Name	Vascular Endothelial Growth Factor Receptor 2
Entrez Gene IDs	3791 (Human); 16542 (Mouse)
Background	VEGF R2/KDR/Flk-1
background_content	Background: VEGF R2/KDR/Flk-1 VEGF R2 (KDR/Flk-1), VEGF R1 (Flt-1), and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGF R1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. Mouse VEGF R2 cDNA encodes a 1367 amino acid (aa) residue precursor protein with a 19 aa residue signal peptide. Mature VEGF R2 is composed of a 743 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 583 aa residue cytoplasmic domain. In contrast to VEGF R1 which binds both PlGF and VEGF with high affinity, VEGF R2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGF R2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist.

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Citations

1. Endothelial deletion of Ino80 disrupts coronary angiogenesis and causes congenital heart disease
   Authors: S Rhee, JI Chung, DA King, G D'amato, DT Paik, A Duan, A Chang, D Nagelberg, B Sharma, Y Jeong, M Diehn, JC Wu, AJ Morrison, K Red-Horse
   Nat Commun, 2018;9(1):368.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


2. VEGFR3 Modulates Vascular Permeability by Controlling VEGF/VEGFR2 Signaling
   Authors: K Heinolaine, S Karaman, G D'Amico, T Tammela, R Sormunen, L Eklund, K Alitalo, G Zarkada
   Circ. Res, 2017;0(0):.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


3. Generation of iPSC-derived limb progenitor-like cells for stimulating phalange regeneration in the adult mouse
   Authors: Y Chen, H Xu, G Lin
   Cell Discov, 2017;3(0):17046.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


4. Impaired angiopoietin/Tie2 signaling compromises Schlemm's canal integrity and induces glaucoma
   Authors: J Kim, DY Park, H Bae, DY Park, D Kim, CK Lee, S Song, TY Chung, DH Lim, Y Kubota, YK Hong, Y He, HG Augustin, G Oliver, GY Koh
   J. Clin. Invest., 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


5. Retinoic acid-induced upregulation of miR-219 promotes the differentiation of embryonic stem cells into neural cells
   Authors: H Wu, J Zhao, B Fu, S Yin, C Song, J Zhang, S Zhao, Y Zhang
   Cell Death Dis, 2017;8(7):e2953.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


6. Cell-matrix signals specify bone endothelial cells during developmental osteogenesis
   Authors: UH Langen, ME Pitulescu, JM Kim, R Enriquez-G, KK Sivaraj, AP Kusumbe, A Singh, J Di Russo, MG Bixel, B Zhou, L Sorokin, JM Vaquerizas, RH Adams
   Nat. Cell Biol, 2017;19(3):189-201.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


7. Pdgfrb-Cre targets lymphatic endothelial cells of both venous and non-venous origins
   Authors: MH Ulvmar, I Martinez-C, L Stanczuk, T Mäkinen
   Genesis, 2016;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Not Specified


8. Semaphorin3A elevates vascular permeability and contributes to cerebral ischemia-induced brain damage.
   Authors: Hou, Sheng Ta, Nilchi, Ladan, Li, Xuesheng, Gangaraju, Sandhya, Jiang, Susan X, Aylsworth, Amy, Monette, Robert, Slinn, Jacqueli
   Sci Rep, 2015;5(0):7890.
   Species: Rat
   Sample Type: Whole Cells
   Application: Bioassay


9. Neural crest-derived SEMA3C activates endothelial NRP1 for cardiac outflow tract septation.
   Authors: Plein A, Calmont A, Fantin A, Denti L, Anderson N, Scambler P, Ruhrberg C
   J Clin Invest, 2015;125(7):2661-76.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Not Specified


10. Slit2 signaling through Robo1 and Robo2 is required for retinal neovascularization.
    Authors: Rama N, Dubrac A, Mathivet T, Ni Charthaigh R, Genet G, Cristofaro B, Pibouin-Fragner L, Ma L, Eichmann A, Chedotal A
    Nat Med, 2015;21(5):483-91.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Not Specified

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