抗VCAM-1抗体(Anti-VCAM-1, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse VCAM‑1/CD106Phe25-Glu698Accession # P29533
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse VCAM‑1/CD106 in direct ELISAs and Western blots.  In direct ELISA and Western blots, approximately 5% cross-reactivity with recombinant human VCAM-1 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.25 µg/mL	See below
Simple Western	1 µg/mL	See below
Flow Cytometry	0.25 µg/106 cells	See below
Immunohistochemistry	5-15 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Western Blot
	Detection of Mouse VCAM‑1/CD106 by Western Blot. Western blot shows lysates of 3T3‑L1 mouse embryonic fibroblast adipose-like cell line and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse VCAM‑1/CD106 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF643) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for VCAM‑1/CD106 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Flow Cytometry
	Detection of VCAM‑1/CD106 in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with Goat Anti-Mouse VCAM‑1/CD106 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF643, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
Immunohistochemistry
	VCAM‑1/CD106 in Mouse Embryo. VCAM‑1/CD106 was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Mouse VCAM‑1/CD106 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF643) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Simple Western
	Detection of Mouse VCAM‑1/CD106 by Simple WesternTM. Simple Western lane view shows lysates of C2C12 mouse myoblast cell line and 3T3‑L1 mouse embryonic fibroblast adipose-like cell line, loaded at 0.2 mg/mL. A specific band was detected for VCAM‑1/CD106 at approximately 116 kDa (as indicated) using 1 µg/mL of Goat Anti-Mouse VCAM‑1/CD106 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF643) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Related Product & Information

Long Name	Vascular Cell Adhesion Molecule 1
Entrez Gene IDs	7412 (Human); 22329 (Mouse)
Background	VCAM-1/CD106
background_content	Background: VCAM-1/CD106 VCAM-1 (CD106), a member of the immunoglobulin superfamily, is a cell surface protein expressed by activated endothelial cells and certain leukocytes (such as macrophages). VCAM-1 expression is induced by IL-1 beta, IL-4, TNF-alpha and IFN-gamma. VCAM-1 binds to leukocyte integrins VLA-4 and alpha 4 beta 7. The human and mouse VCAM-1 proteins share approximately 76% amino acid similarity. During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. They do this by binding to their ligands, for example VCAM-1, on endothelium. The VCAM-1: VLA-4/ alpha 4 beta 7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation. ELISA techniques have shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of VCAM-1 may be elevated or lowered in subjects with a variety of pathological conditions.

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Citations

1. Endothelium-derived extracellular vesicles promote splenic monocyte mobilization in myocardial infarction
   Authors: N Akbar, JE Digby, TJ Cahill, AN Tavare, AL Corbin, S Saluja, S Dawkins, L Edgar, N Rawlings, K Ziberna, E McNeill,, E Johnson, AA Aljabali, RA Dragovic, M Rohling, TG Belgard, IA Udalova, DR Greaves, KM Channon, PR Riley, DC Anthony, RP Choudhury
   JCI Insight, 2017;2(17):.
   Species: Mouse
   Sample Type: Complex Sample Type
   Application: Cell selection


2. Loss of Ptpn11 (Shp2) drives satellite cells into quiescence
   Authors: J Griger, R Schneider, I Lahmann, V Schöwel, C Keller, S Spuler, M Nazaré, C Birchmeier
   Elife, 2017;6(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


3. Suberanilohydroxamic Acid as a Pharmacological Kruppel-Like Factor 2 Activator That Represses Vascular Inflammation and Atherosclerosis
   Authors: Y Xu, S Xu, P Liu, M Koroleva, S Zhang, S Si, ZG Jin
   J Am Heart Assoc, 2017;6(12):.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


4. Numb regulates somatic cell lineage commitment during early gonadogenesis in mice
   Authors: YT Lin, L Barske, T DeFalco, B Capel
   Development, 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


5. Control of the T follicular helper-germinal center B-cell axis by CD8(+) regulatory T cells limits atherosclerosis and tertiary lymphoid organ development.
   Authors: Clement M, Guedj K, Andreata F, Morvan M, Bey L, Khallou-Laschet J, Gaston A, Delbosc S, Alsac J, Bruneval P, Deschildre C, Le Borgne M, Castier Y, Kim H, Cantor H, Michel J, Caligiuri G, Nicoletti A
   Circulation, 2015;131(6):560-70.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC - Not specified


6. High soluble endoglin levels do not induce endothelial dysfunction in mouse aorta.
   Authors: Nemeckova I, Serwadczak A, Oujo B, Jezkova K, Rathouska J, Fikrova P, Varejckova M, Bernabeu C, Lopez-Novoa J, Chlopicki S, Nachtigal P
   PLoS ONE, 2015;10(3):e0119665.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


7. Platelet-driven leukotriene C4-mediated airway inflammation in mice is aspirin-sensitive and depends on T prostanoid receptors.
   Authors: Liu T, Garofalo D, Feng C, Lai J, Katz H, Laidlaw T, Boyce J
   J Immunol, 2015;194(11):5061-8.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


8. Icam-1 targeted nanogels loaded with dexamethasone alleviate pulmonary inflammation.
   Authors: Ferrer M, Shuvaev V, Zern B, Composto R, Muzykantov V, Eckmann D
   PLoS ONE, 2014;9(7):e102329.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


9. CD44-deficiency attenuates the immunologic responses to LPS and delays the onset of endotoxic shock-induced renal inflammation and dysfunction.
   Authors: Rampanelli, Elena, Dessing, Mark C, Claessen, Nike, Teske, Gwendoli, Joosten, Sander P, Pals, Steven T, Leemans, Jaklien, Florquin, Sandrine
   PLoS ONE, 2013;8(12):e84479.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


10. Targeting improves MSC treatment of inflammatory bowel disease.
    Authors: Ko IK, Kim BG, Awadallah A
    Mol. Ther., 2010;18(7):1365-72.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut

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