抗ICAM-1抗体(Anti-ICAM-1, Rat, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Rat
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant rat ICAM-1/CD54Gln28-Thr493Accession # Q00238
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects rat ICAM-1/CD54 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse (rm) ICAM-1 and 5% cross-reactivity with recombinant human (rh) ICAM-1, rhICAM‑3, and rmICAM‑2 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.2 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below
Adhesion Blockade	The adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells (5 x 104 cells/well) to immobilized Recombinant Rat ICAM-1 Fc Chimera (Catalog # 583-IC, 12.5 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 25 µg/mL of the antibody.

Western Blot
	Detection of Rat ICAM‑1/CD54 by Western Blot. Western blot shows lysates of rat liver tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Rat ICAM‑1/CD54 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF583) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for ICAM‑1/CD54 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
	ICAM‑1/CD54 in Rat Brain. ICAM‑1/CD54 was detected in perfusion fixed frozen sections of rat brain using Goat Anti-Rat ICAM‑1/CD54 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF583) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

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Related Product & Information

Long Name	Intercellular Adhesion Molecule 1
Entrez Gene IDs	3383 (Human); 15894 (Mouse); 25464 (Rat)
Background	ICAM-1/CD54
background_content	Background: ICAM-1/CD54 Intercellular Adhesion Molecule-1 (ICAM-1, CD54), binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta, and lipolysaccharide (LPS). Rat and human ICAM-1 share approximately 52% amino acid identity. Rat and mouse ICAM-1 share approximately 52% amino acid identity. Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, leukocyte adhesion deficiency syndrome (LAD), cancer, and transplantation.

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Citations

1. Kv1.3 channel blocker (ImKTx88) maintains blood-brain barrier in experimental autoimmune encephalomyelitis
   Authors: J Huang, S Han, Q Sun, Y Zhao, J Liu, X Yuan, W Mao, B Peng, W Liu, J Yin, X He
   Cell Biosci, 2017;7(0):31.
   Species: Rat
   Sample Type: Whole Tissue
   Application: IHC


2. Effects of aqueous extracts of Taraxacum Officinale on expression of tumor necrosis factor-alpha and intracellular adhesion molecule 1 in LPS-stimulated RMMVECs
   Authors: G Hu, J Wang, D Hong, T Zhang, H Duan, X Mu, Z Yang
   BMC Complement Altern Med, 2017;17(1):38.
   Species: Rat
   Sample Type: Cell Lysates
   Application: WB


3. Sexual dimorphism in the hepatic protein response to a moderate trans fat diet in senescence-accelerated mice
   Authors: SA Bloomer, KE Wellen, GC Henderson
   Lipids Health Dis, 2017;16(1):243.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


4. Astaxanthin Inhibits Expression of Retinal Oxidative Stress and Inflammatory Mediators in Streptozotocin-Induced Diabetic Rats.
   Authors: Yeh P, Huang H, Yang C, Yang W, Yang C
   PLoS ONE, 2016;11(1):e0146438.
   Species: Rat
   Sample Type: Cell Lysates
   Application: WB


5. Sustained elevation of NF-?B activity sensitizes offspring of maternal inflammation to hypertension via impairing PGC-1? recovery
   Sci Rep, 2016;6(0):32642.
   Species: Rat
   Sample Type: Tissue Homogenates
   Application: WB


6. Disrupting the blood-brain barrier by focused ultrasound induces sterile inflammation
   Proc. Natl. Acad. Sci. U.S.A, 2016;0(0):.
   Species: Rat
   Sample Type: Whole Tissue
   Application: IHC


7. Hypothermic preservation of lung allograft inhibits cytokine-induced chemoattractant-1, endothelial leucocyte adhesion molecule, vascular cell adhesion molecule-1 and intracellular adhesion molecule-1 expression.
   Authors: Hanusch C, Nowak K, Gill IS, Torlitz P, Rafat N, Mueller AM, Van Ackern KC, Yard B, Beck GC
   Clin. Exp. Immunol., 2007;149(2):364-71.
   Species: Rat
   Sample Type: Whole Tissue
   Application: IHC


8. Aspirin at low-intermediate concentrations protects retinal vessels in experimental diabetic retinopathy through non-platelet-mediated effects.
   Authors: Sun W, Gerhardinger C, Dagher Z, Hoehn T, Lorenzi M
   Diabetes, 2005;54(12):3418-26.
   Species: Rat
   Sample Type: Whole Tissue
   Application: IHC

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