抗GFRα1抗体(Anti-GFRα1, Rat, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Rat
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant rat GFR alpha ‑1/GDNF R alpha ‑1Asp25-Leu445Accession # Q62997
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects rat GFR alpha ‑1/GDNF R alpha ‑1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human GFR alpha ‑1 is observed and less than 1% cross-reactivity with recombinant mouse GFR alpha ‑2 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.2 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below
Blockade of Receptor-ligand Interaction	In a functional ELISA, 4-10 µg/mL of this antibody will block 50% of the binding of 4 ng/mL of Recombinant Human GDNF (Catalog # 212-GD) to immobilized Recombinant Rat GFR alpha -1 Fc Chimera (Catalog # 560-GR) coated at 0.5 µg/mL (100 µL/well).

Western Blot
	Detection of Rat GFR alpha ‑1/GDNF R alpha ‑1 by Western Blot. Western blot shows lysates of rat brain tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Rat GFR alpha ‑1/GDNF R alpha ‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF560) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for GFR alpha ‑1/GDNF R alpha ‑1 at approximately 52 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
	GFR alpha ‑1/GDNF R alpha ‑1 in Rat Spinal Cord. GFR alpha ‑1/GDNF R alpha ‑1 was detected in perfusion fixed frozen sections of rat spinal cord using Goat Anti-Rat GFR alpha ‑1/GDNF R alpha ‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF560) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to spinal cord dorsal horn. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

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Related Product & Information

Background

GFR alpha-1/GDNF R alpha-1

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Background: GFR alpha-1/GDNF R alpha-1 Glial cell line-derived growth factor (GDNF), neurturin (NTN) and persephin, distant members of the TGF-beta  superfamily, are neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. The bioactivities of GDNF and NTN are mediated through a receptor complex composed of the non ligand-binding signaling subunit (c-Ret receptor tyrosine kinase) and either of two ligand binding subunits (GDNF receptor alpha - (GFR alpha -1) or GFR alpha -2). GFR alpha -1 and‑2 are members of a family of at least four cysteine-rich glycosyl-phosphatidylinositol (GPI)-linked cell surface proteins that share conserved placements of many of their cysteine residues. Binding of GDNF to membrane-associated GFR alpha -1 or GFR alpha -2 initiates the association with and activation of the Ret tyrosine kinase. Soluble GFR alpha s released enzymatically from the cell surface-associated protein with phosphatidylinositol phospholipase C, as well as recombinantly produced soluble GFR alpha -1, can also bind with high-affinity to GDNF and trigger the activation of Ret tyrosine kinase. Rat GFR alpha -1 cDNA encodes a 468 amino acid (aa) residue protein with anN‑terminal 24 aa residue hydrophobic signal peptide. Like other GPI-linked proteins, rat GFR alpha -1 has a C-terminal hydrophobic region which is preceded by a three aa residue (ASS) GPI-binding site. Human GFR alpha -1 shares 93% amino acid identity with rat GFR alpha -1. The expression of the various GFR alpha s are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alpha s in mediating the activities of the GDNF family of neurotrophic factors.

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Citations

1. Advanced immunostaining approaches to study early male germ cell development
   Authors: BA Niedenberg, CB Geyer
   Stem Cell Res, 2018;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC-Fr


2. Novel combinatorial screening identifies neurotrophic factors for selective classes of motor neurons
   Authors: S Schaller, D Buttigieg, A Alory, A Jacquier, M Barad, M Merchant, D Gentien, P de la Gran, G Haase
   Proc. Natl. Acad. Sci. U.S.A, 2017;114(12):E2486-E2493.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


3. Aging-related limit of exercise efficacy on motor decline
   Authors: JC Arnold, MA Cantu, EA Kasanga, VA Nejtek, EV Papa, N Bugnariu, MF Salvatore
   PLoS ONE, 2017;12(11):e0188538.
   Species: Rat
   Sample Type: Tissue Homogenates
   Application: WB


4. Transcriptome analysis reveals transmembrane targets on transplantable midbrain dopamine progenitors.
   Authors: Bye C, Jonsson M, Bjorklund A, Parish C, Thompson L
   Proc Natl Acad Sci U S A, 2015;112(15):E1946-55.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC - Not specified


5. LIN28A marks the spermatogonial progenitor population and regulates its cyclic expansion.
   Authors: Chakraborty P, Buaas F, Sharma M, Snyder E, de Rooij D, Braun R
   Stem Cells, 2014;32(4):860-73.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IF


6. PAX7 expression defines germline stem cells in the adult testis.
   Authors: Aloisio G, Nakada Y, Saatcioglu H, Pena C, Baker M, Tarnawa E, Mukherjee J, Manjunath H, Bugde A, Sengupta A, Amatruda J, Cuevas I, Hamra F, Castrillon D
   J Clin Invest, 2014;124(9):3929-44.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


7. Glial cell line-derived neurotrophic factor (GDNF) induces neuritogenesis in the cochlear spiral ganglion via neural cell adhesion molecule (NCAM).
   Authors: Euteneuer S, Yang K, Chavez E, Leichtle A, Loers G, Olshansky A, Pak K, Schachner M, Ryan A
   Mol Cell Neurosci, 2013;54(0):30-43.
   Species: Rat
   Sample Type:
   Application: WB


8. Retinoic acid availability drives the asynchronous initiation of spermatogonial differentiation in the mouse.
   Authors: Snyder EM, Small C, Griswold MD
   Biol. Reprod., 2010;83(5):783-90.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


9. The heterogeneity of spermatogonia is revealed by their topology and expression of marker proteins including the germ cell-specific proteins Nanos2 and Nanos3.
   Authors: Suzuki H, Sada A, Yoshida S, Saga Y
   Dev. Biol., 2009;336(2):222-31.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


10. Glial cell line-derived neurotrophic factor: an intraovarian factor that enhances oocyte developmental competence in vitro.
    Authors: Linher K, Wu D, Li J
    Endocrinology, 2007;148(9):4292-301.
    Species: Porcine
    Sample Type: Cell Lysates
    Application: WB

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