抗IL-13 Receptor α2抗体(Anti-IL-13 Receptor α2, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse IL‑13 R alpha 2Leu22-Lys334Accession # O88786
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse IL-13 R alpha 2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human (rh) IL-13 R alpha 2 is observed and less than 1% cross-reactivity with rhIL-5R alpha, rhIL-5R beta, rhIL-9 R, and rhIL-4 R is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse IL‑13 R alpha 2 Fc Chimera (Catalog # 539-IR)
Immunohistochemistry	5-15 µg/mL	See below
Immunocytochemistry	5-15 µg/mL	See below

Immunocytochemistry
	IL‑13 R alpha 2 in Mouse Splenocytes. IL‑13 R alpha 2 was detected in immersion fixed mouse splenocytes using Mouse IL‑13 R alpha 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF539) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Immunohistochemistry
	IL‑13 R alpha 2 in Mouse Thymus. IL‑13 R alpha 2 was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse IL‑13 R alpha 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF539) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

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Related Product & Information

Background

IL-13 R alpha 2

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Background: IL-13 R alpha 2 Two type1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha ' or NR4, is now referred to as IL-13 R alpha 1. The high affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2. The mouse IL-13 R alpha 2 cDNA encodes a 383 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide, a 313 residue extracellular domain, a 22 aa residue transmembrane region, and a 27 aa residue cytoplasmic tail. Human and mouse IL-13 R alpha 2 share 59% aa sequence identity. The extracellular domain of IL-13 R alpha 2 is also closely related to that of IL-13 R alpha 1. However, the cytoplasmic domain of IL-13 R alpha 2 lacks the box 1 and box 2 signaling motif and is much shorter than that of IL-13 R alpha 1, suggesting that the two receptors are functionally distinct. IL-13 R alpha 1 has been shown to combine with IL-4 R alpha to form a high-affinity receptor complex capable of transducing both an IL-4-dependent and an IL-13-dependent proliferative signal. The role of IL-13 R alpha 2 in IL-13 signaling remains to be elucidated. The amino-terminal 27 amino acid residues of mouse IL-13 R alpha 2 are identical to that of a soluble mouse IL-13 binding protein purified from mouse serum and urine. Recombinant mouse IL-13 R alpha /Fc chimera has been shown to bind IL-13 with high affinity and is a potent IL-13 antagonist.

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Citations

1. Unexpected involvement of IL-13 signalling via a STAT6 independent mechanism during murine IgG2a development following viral vaccination
   Authors: MA Hamid, RJ Jackson, S Roy, M Khanna, C Ranasinghe
   Eur. J. Immunol., 2018;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


2. Disruption of the TWEAK/Fn14 pathway prevents 5-fluorouracil-induced diarrhea in mice
   Authors: T Sezaki, Y Hirata, T Hagiwara, YI Kawamura, T Okamura, R Takanashi, K Nakano, M Tamura-Nak, LC Burkly, T Dohi
   World J. Gastroenterol., 2017;23(13):2294-2307.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


3. GdCl3 Attenuates Schistosomiasis japonicum Egg-Induced Granulomatosis Accompanied by Decreased Macrophage Infiltration in Murine Liver.
   Authors: Zheng S, Lu Q, Xu Y, Wang X, Shen J, Wang W
   PLoS ONE, 2015;10(8):e0132222.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


4. Matrix metalloproteinase 8 contributes to solubilization of IL-13 receptor alpha2 in vivo.
   Authors: Chen W, Tabata Y, Gibson AM, Daines MO, Warrier MR, Wills-Karp M, Hershey GK
   J. Allergy Clin. Immunol., 2008;122(3):625-32.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


5. Level of expression of IL-13R alpha 2 impacts receptor distribution and IL-13 signaling.
   Authors: Daines MO, Tabata Y, Walker BA, Chen W, Warrier MR, Basu S, Hershey GK
   J. Immunol., 2006;176(12):7495-501.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


6. Loss of pro-apoptotic Bim promotes accumulation of pulmonary T lymphocytes and enhances allergen-induced goblet cell metaplasia.
   Authors: Pierce J, Rir-Sima-Ah J, Estrada I, Wilder J, Strasser A, Tesfaigzi Y
   Am. J. Physiol. Lung Cell Mol. Physiol., 2006;291(5):L862-70.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


7. Impact of interleukin-13 responsiveness on the synthetic and proliferative properties of Th1- and Th2-type pulmonary granuloma fibroblasts.
   Authors: Jakubzick C, Choi ES, Kunkel SL, Joshi BH, Puri RK, Hogaboam CM
   Am. J. Pathol., 2003;162(5):1475-86.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC

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