抗uPA Receptor-1抗体(Anti-uPA Receptor-1, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse uPAR isoform 1Leu24-Thr297Accession # Q545X5
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse uPAR in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human uPAR is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse uPAR Fc Chimera (Catalog # 531-PA)
Flow Cytometry	2.5 µg/106 cells	Mouse whole blood
Immunohistochemistry	5-15 µg/mL	See below
Blockade of Receptor-ligand Interaction	In a functional ELISA, 0.8-4 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of Recombinant Human u-Plasminogen Activator/Urokinase (Catalog # 1310-SE) to immobilized Recombinant Mouse uPAR Fc Chimera (Catalog # 531-PA) coated at 5 µg/mL (100 µL/well). At 25 μg/mL, this antibody will block >90% of the binding.
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunohistochemistry

uPAR in Mouse Kidney. uPAR was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse uPAR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF534) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

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Related Product & Information

Long Name	Urokinase-type Plasminogen Activator Receptor
Entrez Gene IDs	5329 (Human); 18793 (Mouse)
Background	uPAR
background_content	Background: uPAR The urokinase-type plasminogen activator (uPA) is one of two activators that converts the extracellular zymogen plasminogen to plasmin, a serine protease that is involved in a variety of normal and pathological processes that require cell migration and/or tissue destruction. uPA is synthesized and released from cells as a single‑chain (sc) pro-enzyme with limited enzymatic activity and is converted to an active two-chain (tc) disulfide-linked active enzyme by plasmin and other specific proteinases. Both the scuPA and tcuPA bind with high-affinity to the cell surface via the glycosyl phosphatidylinositol-linked receptor uPAR which serves to localize the uPA proteolytic activity. The enzymatic activity of scuPA has also been shown to be enhanced by binding to uPAR. Independent of their proteolytic activity, the uPA/uPAR interaction also initiates signal transduction responses resulting in activation of protein tyrosine kinases, gene expression, cell adhesion, and chemotaxis. uPAR can interact with integrins to suppress normal integrin adhesive function and promote adhesion to vitronectin through a high affinity vitronectin binding site on uPAR. Mouse uPAR-1/Fc cDNA encodes a 327 amino acid (aa) residue precursor protein with a 23 aa residue signal peptide, seven potential N-linked glycosylation sites and a C-terminal GPI-anchor site. An alternate spliced variant of uPAR encoding a secreted soluble form of uPAR also exists. Human and mouse uPAR share approximately 60% aa sequence identity and the receptor-ligand interaction is highly species-specific. Human uPA binds rmuPAR at a lower affinity compared to rhuPAR.

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Citations

1. Cleavage of the urokinase receptor (uPAR) on oral cancer cells: regulation by transforming growth factor - ?1 (TGF-?1) and potential effects on migration and invasion
   Authors: SN Magnussen, E Hadler-Ols, DE Costea, E Berg, CC Jacobsen, B Mortensen, T Salo, I Martinez-Z, JO Winberg, L Uhlin-Hans, G Svineng
   BMC Cancer, 2017;17(1):350.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


2. Urokinase-type Plasminogen Activator (uPA) Binding to the uPA Receptor (uPAR) Promotes Axonal Regeneration in the Central Nervous System
   Authors: Paola Merino
   J. Biol. Chem, 2016;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


3. A reassessment of soluble urokinase-type plasminogen activator receptor in glomerular disease.
   Authors: Spinale J, Mariani L, Kapoor S, Zhang J, Weyant R, Song P, Wong H, Troost J, Gadegbeku C, Gipson D, Kretzler M, Nihalani D, Holzman L
   Kidney Int, 2015;87(3):564-74.
   Species: Mouse
   Sample Type: Serum
   Application: ELISA detection


4. Tumour microenvironments induce expression of urokinase plasminogen activator receptor (uPAR) and concomitant activation of gelatinolytic enzymes.
   Authors: Magnussen S, Hadler-Olsen E, Latysheva N, Pirila E, Steigen S, Hanes R, Salo T, Winberg J, Uhlin-Hansen L, Svineng G
   PLoS ONE, 2014;9(8):e105929.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


5. Antipermeability function of PEDF involves blockade of the MAP kinase/GSK/beta-catenin signaling pathway and uPAR expression.
   Authors: Yang J, Duh EJ, Caldwell RB, Behzadian MA
   Invest. Ophthalmol. Vis. Sci., 2010;51(6):3273-80.
   Species: Bovine
   Sample Type: Cell Culture Supernates
   Application: WB


6. Urokinase-type plasminogen activator receptor (uPAR) augments brain damage in a murine model of ischemic stroke.
   Authors: Nagai N, Okada K, Kawao N, Ishida C, Ueshima S, Collen D, Matsuo O
   Neurosci. Lett., 2007;432(1):46-9.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: IP


7. Inhibition of choroidal neovascularization by a peptide inhibitor of the urokinase plasminogen activator and receptor system in a mouse model.
   Authors: Das A, Boyd N, Jones TR, Talarico N, McGuire PG
   Arch. Ophthalmol., 2004;122(12):1844-9.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


8. Mitogenic signaling of urokinase receptor-deficient kidney fibroblasts: actions of an alternative urokinase receptor and LDL receptor-related protein.
   Authors: Zhang G, Cai X, Lopez-Guisa JM, Collins SJ, Eddy AA
   J. Am. Soc. Nephrol., 2004;15(8):2090-102.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB

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