抗TARC抗体(Anti-TARC, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse CCL17/TARCAla24-Pro93Accession # Q9WUZ6
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse CCL17/TARC in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human TARC is observed. Neutralizes the biological activity of recombinant mouse TARC, and will also neutralize the biological activity of recombinant human TARC using a 20 fold greater Ig concentration.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse CCL17/TARC (Catalog # 529-TR)
Neutralization	Measured by its ability to neutralize CCL17/TARC-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR4. The Neutralization Dose (ND50) is typically 0.2-0.6 µg/mL in the presence of 0.02 µg/mL Recombinant Mouse CCL17/TARC.

Neutralization

Chemotaxis Induced by CCL17/TARC and Neutralization by Mouse CCL17/TARC Antibody. Recombinant Mouse CCL17/TARC (Catalog # 529‑TR) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL17/TARC (0.02 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL17/TARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF529). The ND50 is typically0.2-0.6 µg/mL.

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Related Product & Information

Entrez Gene IDs	6361 (Human); 20295 (Mouse)
Background	CCL17/TARC
background_content	Background: CCL17/TARC Human thymus and activation-regulated chemokine (TARC) also known as CCL17, is a novel CC chemokine identified using a signal sequence trap method. Mouse TARC was discovered as a dendritic cell (DC) specific gene by differentiation RNA display. Mouse TARC cDNA encodes a highly basic 93 amino acid (aa) residue precursor protein with a 23 aa residue putative signal peptide that is cleaved to generate the 70 aa residue mature secreted protein. Among CC chemokine family members, TARC has approximately 24 - 29% amino acid sequence identity with RANTES, MIP-1 alpha, MIP-1 beta, MCP-1, MCP-2, MCP-3 and I-309. The gene for human TARC has been mapped to chromosome 16q13 rather than chromosome 17 where the genes for many human CC chemokines are clustered. Mouse TARC is constitutively expressed in thymic DC, and at a lower level in lymph node DC in the lung. Recombinant TARC has been shown to be chemotactic for T cell lines and antigen-primed T helper cells. In humans, TARC was identified to be a specific functional ligand for CCR-4 and CCR-8, receptors that are selectively expressed on T cells.

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Citations

1. IL-4/CCL22/CCR4 axis controls regulatory T-cell migration that suppresses inflammatory bone loss in murine experimental periodontitis.
   Authors: Araujo-Pires A, Vieira A, Francisconi C, Biguetti C, Glowacki A, Yoshizawa S, Campanelli A, Trombone A, Sfeir C, Little S, Garlet G
   J Bone Miner Res, 2015;30(3):412-22.
   Species: Mouse
   Sample Type: In Vivo
   Application: Neut


2. Defective immunoregulation in RSV vaccine-augmented viral lung disease restored by selective chemoattraction of regulatory T cells.
   Authors: Loebbermann, Jens, Durant, Lydia, Thornton, Hannah, Johansson, Cecilia, Openshaw, Peter J
   Proc Natl Acad Sci U S A, 2013;110(8):2987-92.
   Species: Mouse
   Sample Type: In Vivo
   Application: Neut


3. Comprehensive assessment of chemokine expression profiles by flow cytometry.
   Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
   J. Clin. Invest., 2010;120(3):907-23.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


4. Allergic pulmonary inflammation in mice is dependent on eosinophil-induced recruitment of effector T cells.
   Authors: Jacobsen EA, Ochkur SI, Pero RS, Taranova AG, Protheroe CA, Colbert DC, Lee NA, Lee JJ
   J. Exp. Med., 2008;205(3):699-710.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


5. CCL17 transgenic mice show an enhanced Th2-type response to both allergic and non-allergic stimuli.
   Authors: Tsunemi Y, Saeki H, Nakamura K, Nagakubo D, Nakayama T, Yoshie O, Kagami S, Shimazu K, Kadono T, Sugaya M, Komine M, Matsushima K, Tamaki K
   Eur. J. Immunol., 2006;36(8):2116-27.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


6. Immunosuppressive effects of CCL17 on pulmonary antifungal responses during pulmonary invasive aspergillosis.
   Authors: Carpenter KJ, Hogaboam CM
   Infect. Immun., 2005;73(11):7198-207.
   Species: Mouse
   Sample Type:
   Application: Neut

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