抗HGF Receptor抗体(Anti-HGF Receptor, c-Met, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse HGF R/c-METGlu25-Asn929Accession # P16056
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse HGF R/c-MET in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) HGF R and less than 1% cross-reactivity with rhMSP R is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse HGF R/c-MET Fc Chimera (Catalog # 527-ME)
Immunohistochemistry	5-15 µg/mL	See below
Blockade of Receptor-ligand Interaction	In a functional ELISA, 0.3-1 µg/mL of this antibody will block 50% of the binding of 5 ng/mL of Recombinant Human HGF (Catalog # 256-GF) to immobilized Recombinant Mouse HGF R/c-MET Fc Chimera (Catalog # 527‑ME) coated at 1 µg/mL (100 µL/well). At 20 μg/mL, this antibody will block >90% of the binding.
Immunocytochemistry	5-15 µg/mL	See below

Immunocytochemistry
	HGF R/c‑MET in HT‑29 and U937 Human Cell Lines. HGF R/c‑MET was detected in immersion fixed HT‑29 human colon adenocarcinoma cell line (positive control, left panel) and U937 human histiocytic lymphoma cell line (negative control, right panel) using Goat Anti-Mouse HGF R/c‑MET Antigen Affinity-purified Polyclonal Antibody (Catalog # AF527) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
	HGF R/c‑MET in Mouse Embryo. HGF R/c‑MET was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Mouse HGF R/c‑MET Antigen Affinity-purified Polyclonal Antibody (Catalog # AF527) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

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Related Product & Information

Long Name	Hepatocyte Growth Factor Receptor
Entrez Gene IDs	4233 (Human); 17295 (Mouse)
Background	HGF R/c-MET
background_content	Background: HGF R/c-MET HGF R, also known as Met (from N-methyl-N’-nitro-N-nitrosoguanidine induced), is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. HGF R is synthesized as a single chain precursor which undergoes cotranslational proteolytic cleavage. This generates a mature HGF R that is a disulfide-linked dimer composed of a 50 kDa extracellular  alpha chain and a 145 kDa transmembrane beta chain (1, 2). The extracellular domain (ECD) contains a seven bladed beta -propeller sema domain, a cysteine-rich PSI/MRS, and four Ig-like E-set domains, while the cytoplasmic region includes the tyrosine kinase domain (3, 4). An alternately spliced form of mouse HGF R lacks a cytoplasmic juxtamembrane region important for regulation of signal transduction (5, 6). The sema domain, which is formed by both the alpha and beta chains of HGF R, mediates both ligand binding and receptor dimerization (3, 7). Ligand-induced tyrosine phosphorylation in the cytoplasmic region activates the kinase domain and provides docking sites for multiple SH2-containing molecules (8, 9). HGF stimulation induces HGF R downregulation via internalization and proteasome-dependent degradation (10). In the absence of ligand, HGF R forms non-covalent complexes with a variety of membrane proteins including CD44v6, CD151, EGF R, Fas, integrin  alpha 6/ beta 4, plexins B1, 2, 3, and MSP R/Ron (11-18). Ligation of one complex component triggers activation of the other, followed by cooperative signaling effects (11-18). Formation of some of these heteromeric complexes is a requirement for epithelial cell morphogenesis and tumor cell invasion (11, 15, 16). Paracrine induction of epithelial cell scattering and branching tubulogenesis results from the stimulation of HGF R on undifferentiated epithelium by HGF released from neighboring mesenchymal cells (19). Genetic polymorphisms, chromosomal translocation,over-expression, and additional splicing and proteolytic cleavage of HGF R have been described in a wide range of cancers (1). Within the ECD, mouse HGF R shares 87%, 87%, and 94% amino acid sequence identity with canine, human, and rat HGF R, respectively.

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Citations

1. Receptor Tyrosine Kinase Met Promotes Cell Survival via Kinase-Independent Maintenance of Integrin ?3?1
   Authors: Lia Tesfay
   Mol Biol Cell, 2016;0(0):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


2. Beta 1-integrin-c-Met cooperation reveals an inside-in survival signalling on autophagy-related endomembranes
   Authors: Rachel Barrow-McG
   Nat Commun, 2016;7(0):11942.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


3. Hepatocyte Growth Factor-c-MET Signaling Mediates the Development of Nonsensory Structures of the Mammalian Cochlea and Hearing
   J Neurosci, 2016;36(31):8200-9.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC - Frozen


4. MET is required for the recruitment of anti-tumoural neutrophils.
   Authors: Finisguerra V, Di Conza G, Di Matteo M, Serneels J, Costa S, Thompson A, Wauters E, Walmsley S, Prenen H, Granot Z, Casazza A, Mazzone M
   Nature, 2015;522(7556):349-53.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: ELISA Capture


5. Regulation of hepatocyte growth factor in mice with pneumonia by peptidases and trans-alveolar flux.
   Authors: Raymond W, Xu X, Nimishakavi S, Le C, McDonald D, Caughey G
   PLoS ONE, 2015;10(5):e0125797.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC OCT-embedded


6. Targeting matriptase in breast cancer abrogates tumour progression via impairment of stromal-epithelial growth factor signalling.
   Authors: Zoratti G, Tanabe L, Varela F, Murray A, Bergum C, Colombo E, Lang J, Molinolo A, Leduc R, Marsault E, Boerner J, List K
   Nat Commun, 2015;6(0):6776.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


7. Recombinant insulin-like growth factor-1 activates satellite cells in the mouse urethral rhabdosphincter.
   Authors: Wei W, Howard P, Macarak E
   BMC Urol, 2013;13(0):62.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


8. Enhanced c-Met activity promotes G-CSF-induced mobilization of hematopoietic progenitor cells via ROS signaling.
   Authors: Tesio M, Golan K, Corso S
   Blood, 2011;117(2):419-28.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


9. c-Met and its ligand hepatocyte growth factor/scatter factor regulate mature B cell survival in a pathway induced by CD74.
   Authors: Gordin M, Tesio M, Cohen S
   J. Immunol., 2010;185(4):2020-31.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


10. Expression of the HGF receptor c-met by macrophages in experimental autoimmune encephalomyelitis.
    Authors: Moransard M, Sawitzky M, Fontana A, Suter T
    Glia, 2010;58(5):559-71.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: IP

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