抗IL-18抗体(Anti-IL-18, Rat, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Rat
Label	Unconjugated
Immunogen	E. coli-derived recombinant rat IL‑18/IL-1F4His37-Ser194Accession # P97636
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects rat IL‑18/IL-1F4 in direct ELISAs and Western blots. In Western blots, approximately 35% cross-reactivity with recombinant mouse IL‑18 is observed and approximately 10% cross-reactivity with recombinant human IL‑18 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Rat IL‑18/IL‑1F4 (Catalog # 521-RL)
Neutralization	Measured by its ability to neutralize IL‑18/IL‑1F4-induced IFN‑ gamma secretion in activated mouse T cells [Ahn, H.J. et al. (1997) J. Immunol. 159:2125]. The Neutralization Dose (ND50) is typically 1-3 µg/mL in the presence of 15 ng/mL Recombinant Rat IL‑18/IL‑1F4 and 0.1 ng/mL Recombinant Mouse IL‑12.

Neutralization

IFN‑ gamma Secretion Induced by IL‑18/IL‑1F4 and Neutralization by Rat IL‑18/IL‑1F4 Antibody. In the presence of Recombinant Mouse IL‑12 (0.1 ng/mL, Catalog # 419‑ML), Recombinant Rat IL‑18/IL‑1F4 (Catalog # 521‑RL) stimulates IFN‑ gamma secretion in activated mouse T cells in a dose-dependent manner (orange line), as measured by the Mouse IFN‑ gamma Quantikine ELISA Kit (Catalog # MIF00). Under these conditions, IFN‑ gamma secretion elicited by Recombinant Rat IL‑18/IL‑1F4 (15 ng/mL) is neutralized (green line) by increasing concentrations of Rat IL‑18/IL‑1F4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF521). The ND50 is typically 1-3 µg/mL.

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Related Product & Information

Long Name	Interleukin 18
Entrez Gene IDs	3606 (Human); 16173 (Mouse); 29197 (Rat); 397057 (Porcine); 574151 (Primate)
Background	IL-18/IL-1F4
background_content	Background: IL-18/IL-1F4 Interleukin-18 (IL-18), also known as IL-1F4 and IFN-gamma inducing factor (IGIF), is a member of the IL-1 family of cytokines and is a key molecule in the innate immune response (1). Rat IL-18 is synthesized as a 24 kDa proprotein that contains a 36 amino acid (aa) propeptide and a 158 aa mature region (2). Under inflammatory conditions, the propeptide is cleaved by Caspase-1 in the cytoplasm to liberate the mature nonglycosylated 18 kDa monomeric IL-18 (3, 4). Mature rat IL-18 shares 91% aa sequence identity with mouse IL-18 and 60-64% aa sequence identity with human, canine, feline, porcine, and rhesus macaque IL-18. IL-18 is secreted by a variety of cell types including macrophages, dendritic cells, and epithelial cells (1, 5). Circulating mature IL-18 is sequestered by soluble IL-18 binding proteins (IL-18 BP) that inhibit IL-18 bioactivity (6). IL-18 interacts with the widely expressed IL-18 R alpha which then recruits the signaling subunit IL-18 R beta (7, 8). The IL-1 family member IL-1F7 also binds to IL-18 R alpha but does not recruit IL-18 R beta or induce signaling (9). IL-1F7 binds IL-18 BP and enhances its neutralizing effect on IL-18 activity (9). IL-18 synergizes with other cytokines to activate NK, Th1, and Th17 cells and to increase the production of IFN-gamma (1, 5, 10-12). IL-18 can also promote Th2 cytokine release which reduces the effectiveness of antiviral responses (13, 14). Increased levels of active IL-18 contribute to the severity of autoimmunity and hypertension, while deficiency of IL-18 results in symptoms of metabolic syndrome (1, 5, 15, 16). In cancer, IL-18 stimulates Th1 and NK cells to target tumor cells, but it can also promote angiogenesis, metastasis, and tumor cell immune evasion (11).

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Citations

1. Interleukin-18 and its receptor are expressed in gonadotropin-releasing hormone neurons of mouse and rat forebrain
   Authors: S Kuwahara-O, S Maeda, K Kobayashi, Y Minato, K Tanaka, K Yamanishi, M Hata, W Li, T Hayakawa, K Noguchi, H Okamura, H Yagi
   Neurosci. Lett., 2017;0(0):.
   Species: Rat
   Sample Type: Whole Tissue
   Application: IHC


2. Interleukin-18-mediated microglia/astrocyte interaction in the spinal cord enhances neuropathic pain processing after nerve injury.
   Authors: Miyoshi K, Obata K, Kondo T, Okamura H, Noguchi K
   J. Neurosci., 2008;28(48):12775-87.
   Species: Rat
   Sample Type: In Vivo
   Application: In vivo


3. Inhibition of angiogenesis by interleukin-4 gene therapy in rat adjuvant-induced arthritis.
   Authors: Haas CS, Amin MA, Allen BB, Ruth JH, Haines GK, Woods JM, Koch AE
   Arthritis Rheum., 2006;54(8):2402-14.
   Species: Rat
   Sample Type: Tissue Homogenates
   Application: Neut


4. Caspase-1-processed interleukins in hyperoxia-induced cell death in the developing brain.
   Authors: Felderhoff-Mueser U, Sifringer M, Polley O, Dzietko M, Leineweber B, Mahler L, Baier M, Bittigau P, Obladen M, Ikonomidou C, Buhrer C
   Ann. Neurol., 2005;57(1):50-9.
   Species: Rat
   Sample Type: Tissue Homogenates
   Application: WB


5. Role of IL-18 in acute lung inflammation.
   Authors: Jordan JA, Guo RF, Yun EC, Sarma V, Warner RL, Crouch LD, Senaldi G, Ulich TR, Ward PA
   J. Immunol., 2001;167(12):7060-8.
   Species: Rat
   Sample Type: BALF
   Application: ELISA Development

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