抗TrkB抗体(Anti-TrkB, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse TrkBCys32-His429Accession # P15209
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse TrkB in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant human (rh) TrkB is observed, and less than 2% cross-reactivity with rhTrkA, recombinant rat TrkA, and recombinant mouse TrkC is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.5 µg/mL	See below
Simple Western	10 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below
Blockade of Receptor-ligand Interaction	In a functional ELISA, 0.5-2 µg/mL of this antibody will block 50% of the binding of 5 ng/mL of biotinylated recombinant Human BDNF to immobilized Recombinant Mouse TrkB (Catalog # 1494-TB) coated at 2 µg/mL (100 µL/well). At 50 μg/mL, this antibody will block >90% of the binding.

Western Blot
	Detection of Mouse TrkB by Western Blot. Western blot shows lysates of mouse brain (cerebellum) tissue and mouse brain (cortex) tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse TrkB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1494) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for TrkB at approximately 90-100 and 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
	TrkB in Mouse Spinal Cord. TrkB was detected in perfusion fixed frozen sections of mouse spinal cord using Goat Anti-Mouse TrkB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1494) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to neuronal processes and cell bodies. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Simple Western
	Detection of Mouse TrkB by Simple WesternTM. Simple Western lane view shows lysates of mouse brain (cortex) tissue, loaded at 0.2 mg/mL. A specific band was detected for TrkB at approximately 154 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse TrkB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1494) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Related Product & Information

Long Name	Neurotrophic Tyrosine Kinase Receptor B
Entrez Gene IDs	4915 (Human); 18212 (Mouse); 25054 (Rat)
Background	TrkB
background_content	Background: TrkB The neurotrophins, including NGF, BDNF, NT-3, and NT-4/5, constitute a group of structurally related, secreted proteins that play an important role in the development and function of the nervous system. The biological activities of the neurotrophins are mediated by binding to and activating two unrelated receptor types: the p75 neurotrophin receptor (p75NTR) and the Trk family of receptor tyrosine kinases (1, 2). p75NTR is a member of the tumor necrosis factor receptor superfamily (TNFRSF) and has been designated TNFRSF16. It binds all neurotrophins with low affinity to transduce cellular signaling pathways that synergize with or antagonize those activated by the Trk receptors. Three Trk family proteins, TrkA, TrkB, and TrkC, exhibiting different ligand specificities, have been identified. TrkA binds NGF and NT-3, TrkB binds BDNF, NT-3, and NT-4/5, and TrkC only binds NT-3 (1-2). All Trk family proteins share a conserved, complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain (3). Natural splice variants of the different Trks, lacking the first cysteine-rich domain, the first and second or all three of the leucine-rich motifs, or the tyrosine kinase domain, have been described (4). At the protein sequence level, human and mouse TrkB show 94% amino acid sequence identity (5-6). The proteins also exhibit cross-species activity. The primary location of TrkB expression is in the central and peripheral nervous systems. Low level TrkB expression has also been observed in a wide variety of tissues outside the nervous system (6).

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Citations

1. Protection of nigral dopaminergic neurons by AAV1 transduction with Rheb(S16H) against neurotoxic inflammation in vivo
   Authors: S Kim, GJ Moon, YS Oh, J Park, WH Shin, JY Jeong, KS Choi, BK Jin, N Kholodilov, RE Burke, HJ Kim, CM Ha, SG Lee, SR Kim
   Exp. Mol. Med., 2018;50(2):e440.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


2. Phenotypic and Functional Characterization of Peripheral Sensory Neurons derived from Human Embryonic Stem Cells
   Authors: AJ Alshawaf, S Viventi, W Qiu, G D'Abaco, B Nayagam, M Erlichster, G Chana, I Everall, J Ivanusic, E Skafidas, M Dottori
   Sci Rep, 2018;8(1):603.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


3. Pericytes of Multiple Organs Do Not Behave as Mesenchymal Stem Cells In�Vivo
   Authors: N Guimar?es-, P Cattaneo, Y Sun, T Moore-Morr, Y Gu, ND Dalton, E Rockenstei, E Masliah, KL Peterson, WB Stallcup, J Chen, SM Evans
   Cell Stem Cell, 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC - Frozen


4. Genetic Tracing of Cav3.2 T-Type Calcium Channel Expression in the Peripheral Nervous System
   Authors: YA Bernal Sie, J Haseleu, A Kozlenkov, V Bégay, GR Lewin
   Front Mol Neurosci, 2017;10(0):70.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


5. Development of a pharmacodynamic biomarker to measure target engagement from inhibition of the NGF-TrkA pathway
   Authors: EA Price, A Krasowska-, KK Nanda, SJ Stachel, DA Henze
   J. Neurosci. Methods, 2017;282(0):34-42.
   Species: Rat
   Sample Type: Tissue Homogenates
   Application: ELISA Capture


6. Treadmill exercise suppressed stress-induced dendritic spine elimination in mouse barrel cortex and improved working memory via BDNF/TrkB pathway
   Authors: K Chen, L Zhang, M Tan, CS Lai, A Li, C Ren, KF So
   Transl Psychiatry, 2017;7(3):e1069.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


7. Merkel Cell-Driven BDNF Signaling Specifies SAI Neuron Molecular and Electrophysiological Phenotypes
   Authors: EG Reed-Geagh, MC Wright, LA See, PC Adelman, KH Lee, HR Koerber, SM Maricich
   J Neurosci, 2016;36(15):4362-76.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC - Frozen


8. KCNQ Potassium Channels Modulate Sensitivity of Skin Down-hair (D-hair) Mechanoreceptors
   Authors: S Schütze, IJ Orozco, TJ Jentsch
   J. Biol. Chem, 2016;291(11):5566-75.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


9. Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing.
   Authors: Usoskin D, Furlan A, Islam S, Abdo H, Lonnerberg P, Lou D, Hjerling-Leffler J, Haeggstrom J, Kharchenko O, Kharchenko P, Linnarsson S, Ernfors P
   Nat Neurosci, 2015;18(1):145-53.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


10. Protein tyrosine phosphatase receptor type O inhibits trigeminal axon growth and branching by repressing TrkB and Ret signaling.
    Authors: Gatto G, Dudanova I, Suetterlin P, Davies A, Drescher U, Bixby J, Klein R
    J Neurosci, 2013;33(12):5399-410.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen

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