抗MIG抗体(Anti-MIG, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Data Examples
• Related Research Areas
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse CXCL9/MIGAccession # P18340
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse CXCL9/MIG in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross-reactivity with recombinant human (rh) CXCL9/MIG is observed and less than 0.005% cross-reactivity with recombinant mouse MIP-2, recombinant rat CINC-1, rhGRO alpha, rhGRO beta, and rhGRO gamma is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.5 µg/mL	See below
Mouse CXCL9/MIG Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	0.2-0.8 µg/mL	Mouse CXCL9/MIG Antibody (Catalog #AF-492-NA )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Mouse CXCL9/MIG Biotinylated Antibody (Catalog #BAF492 )
ELISA Standard		Recombinant Mouse CXCL9/MIG Protein (Catalog #492-MM )
Neutralization	Measured by its ability to neutralize CXCL9/MIG-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CXCR3. The Neutralization Dose (ND50) is typically 6-20 µg/mL in the presence of 1 µg/mL Recombinant Mouse CXCL9/MIG.
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Data Examples

Western Blot	click image to view larger	Detection of Mouse CXCL9/MIG by Western Blot. Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse CXCL9/MIG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-492-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CXCL9/MIG at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Neutralization	click image to view larger	Chemotaxis Induced by CXCL9/MIG and Neutralization by Mouse CXCL9/MIG Antibody.Recombinant Mouse CXCL9/MIG (Catalog # 492-MM) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL9/MIG (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL9/MIG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-492-NA). The ND50 is typically 6-20 µg/mL.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL9/MIG	CXCL9, also known as MIG, is a member of the alpha  subfamily of chemokines that lacks the ELR domain, and was initially identified as a lymphokine-activated gene in mouse macrophages. Human CXCL9 was subsequently cloned using mouse CXCL9 cDNA as a probe. The CXCL9 gene is induced in macrophages and in primary glial cells of the central nervous system specifically in response to IFN-gamma. CXCL9 has been shown to be a chemoattractant for activated T-lymphocytes and TIL but not for neutrophils or monocytes. The mouse CXCL9 cDNA encodes a 126 amino acid residue precursor protein with a 21 amino acid residue signal peptide that is cleaved to yield a 105 amino acid residue mature protein. CXCL9 has an extended carboxy-terminus containing greater than 50% basic amino acid residues and is larger than most other chemokines. The carboxy-terminal residues of CXCL9 are prone to proteolytic cleavage resulting in size heterogeneity of natural and recombinant CXCL9. CXCL9 with large carboxy-terminal deletions have been shown to have diminished activity in the calcium flux assay. A chemokine receptor (CXCR3) specific for CXCL9 and IP-10 has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes.
Entrez Gene IDs:	4283 (Human); 17329 (Mouse); 246759 (Rat)
Alternate Names:	chemokine (C-X-C motif) ligand 9; CMK; crg-10; C-X-C motif chemokine 9; CXCL9; Gamma-interferon-induced monokine; Humig; MIG; MIGSmall-inducible cytokine B9; monokine induced by gamma interferon; SCYB9Monokine induced by interferon-gamma

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Related Research Areas

Cancer Biomarkers

Chemokines and Receptors

Chemokines and Receptors in Angiogenesis

CXC Chemokines and Receptors

Inflammatory Mediators

Macrophage Activation Markers

Molecules Secreted by VSMC

Regulatory T Cells (Tregs)

Herpes simplex virus-1 evasion of CD8+ T cell accumulation contributes to viral encephalitis Authors: N Koyanagi, T Imai, K Shindo, A Sato, W Fujii, T Ichinohe, N Takemura, S Kakuta, S Uematsu, H Kiyono, Y Maruzuru, J Arii, A Kato, Y Kawaguchi J. Clin. Invest., 2017;0(0):. Species: Mouse Sample Type: Cell Lysates Application: WB

Western Blot
	Detection of Mouse CXCL9/MIG by Western Blot. Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse CXCL9/MIG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-492-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CXCL9/MIG at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Neutralization
	Chemotaxis Induced by CXCL9/MIG and Neutralization by Mouse CXCL9/MIG Antibody. Recombinant Mouse CXCL9/MIG (Catalog #492-MM) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL9/MIG (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL9/MIG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-492-NA). The ND50 is typically 6-20 µg/mL.

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Related Product & Information

Entrez Gene IDs	4283 (Human); 17329 (Mouse); 246759 (Rat)
Background	CXCL9/MIG
background_content	Background: CXCL9/MIG CXCL9, also known as MIG, is a member of the alpha  subfamily of chemokines that lacks the ELR domain, and was initially identified as a lymphokine-activated gene in mouse macrophages. Human CXCL9 was subsequently cloned using mouse CXCL9 cDNA as a probe. The CXCL9 gene is induced in macrophages and in primary glial cells of the central nervous system specifically in response to IFN-gamma. CXCL9 has been shown to be a chemoattractant for activated T-lymphocytes and TIL but not for neutrophils or monocytes. The mouse CXCL9 cDNA encodes a 126 amino acid residue precursor protein with a 21 amino acid residue signal peptide that is cleaved to yield a 105 amino acid residue mature protein. CXCL9 has an extended carboxy-terminus containing greater than 50% basic amino acid residues and is larger than most other chemokines. The carboxy-terminal residues of CXCL9 are prone to proteolytic cleavage resulting in size heterogeneity of natural and recombinant CXCL9. CXCL9 with large carboxy-terminal deletions have been shown to have diminished activity in the calcium flux assay. A chemokine receptor (CXCR3) specific for CXCL9 and IP-10 has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes.

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Citations

1. Herpes simplex virus-1 evasion of CD8+ T cell accumulation contributes to viral encephalitis
   Authors: N Koyanagi, T Imai, K Shindo, A Sato, W Fujii, T Ichinohe, N Takemura, S Kakuta, S Uematsu, H Kiyono, Y Maruzuru, J Arii, A Kato, Y Kawaguchi
   J. Clin. Invest., 2017;0(0):.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


2. IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3
   Authors: R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie
   Cell. Physiol. Biochem., 2017;42(5):1961-1972.
   Species: Mouse
   Sample Type: Serum
   Application: ELISA Capture


3. Salt suppresses IFN? inducible chemokines through the IFN?-JAK1-STAT1 signaling pathway in proximal tubular cells
   Authors: Y Arai, D Takahashi, K Asano, M Tanaka, M Oda, SBH Ko, MSH Ko, S Mandai, N Nomura, T Rai, S Uchida, E Sohara
   Sci Rep, 2017;7(0):46580.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


4. Optimal CD4 T cell priming after LPS-based adjuvanticity with CD134 costimulation relies on CXCL9 production
   Authors: P Shinde, W Liu, A Ménoret, AD Luster, AT Vella
   J. Leukoc. Biol., 2017;0(0):.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


5. A Novel PD-L1-targeting Antagonistic DNA Aptamer With Antitumor Effects
   Authors: Wei-Yun Lai
   Mol Ther Nucleic Acids, 2016;5(12):e397.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


6. CXCR3 Blockade Inhibits T Cell Migration into the Skin and Prevents Development of Alopecia Areata
   J Immunol, 2016;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


7. Osteoblasts secrete Cxcl9 to regulate angiogenesis in bone
   Nat Commun, 2016;7(0):13885.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


8. Regulatory T cells prevent inducible BALT formation by dampening neutrophilic inflammation.
   Authors: Foo S, Zhang V, Lalwani A, Lynch J, Zhuang A, Lam C, Foster P, King C, Steptoe R, Mazzone S, Sly P, Phipps S
   J Immunol, 2015;194(9):4567-76.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


9. Tumor cell-activated CARD9 signaling contributes to metastasis-associated macrophage polarization.
   Authors: Yang M, Shao J, Miao Y, Cui W, Qi Y, Han J, Lin X, Du J
   Cell Death Differ, 2014;21(8):1290-302.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


10. Trafficking of high avidity HER-2/neu-specific T cells into HER-2/neu-expressing tumors after depletion of effector/memory-like regulatory T cells.
    Authors: Weiss VL, Lee TH, Song H, Kouo TS, Black CM, Sgouros G, Jaffee EM, Armstrong TD
    PLoS ONE, 2012;7(2):e31962.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded

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