抗IL-10 Receptor抗体(Anti-IL-10 Receptor, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

目次に戻る

Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL‑10 R alpha Thr24-Asn238 (Lys188Arg and Glu222Gly)Accession # Q61727
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse IL-10 R alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 25% cross-reactivity with recombinant human IL-10 R alpha is observed.

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse IL‑10 R alpha (Catalog # 474-MR)
Neutralization	Measured by its ability to neutralize IL‑10-induced proliferation in the MC/9‑2 mouse mast cell line [Thompson-Snipes, L.  et al. (1991) J. Exp. Med. 173:507]. The Neutralization Dose (ND50) is typically 1-3 µg/mL in the presence of 1 ng/mL Recombinant Mouse IL‑10.

Neutralization

Cell Proliferation Induced by IL‑10 and Neutralization by Mouse IL‑10 R alpha Antibody. Recombinant Mouse IL‑10 (Catalog # 417-ML) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑10 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑10 R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-474-NA). The ND50 is typically 1-3 µg/mL.

目次に戻る

Related Product & Information

Long Name	Interleukin 10 Receptor alpha
Entrez Gene IDs	3587 (Human); 16154 (Mouse)
Background	IL-10 R alpha
background_content	Background: IL-10 R alpha IL-10, initially designated cytokine synthesis inhibitory factor (CSIF), is a potent immunosuppressant of macrophage functions. IL-10 is also a pleiotropic cytokine with multiple immunostimulatory as well as immunosuppressive effects on a variety of other cell types. IL-10 binds specifically and with high affinity to cell-surface receptors. Mouse and human cDNA clones encoding the ligand-binding IL-10 receptor (IL-10 R) have been isolated. The IL-10 R mRNA has been detected in all cell types that are known to respond to IL-10. Human and mouse IL-10 receptors are structurally related to the IFN-gamma receptor. These receptors are members of the class II subgroup of the cytokine receptor superfamily. The deduced amino acid sequence of human IL-10 R is approximately 60% identical to mouse IL-10 R. Although human IL-10 has cross-species activities and is active on mouse cells, mouse IL-10 is species-specific in its actions and does not bind to the human IL-10 receptor. The human IL-10 R gene has been mapped to chromosome 11q23.3. Recombinant IL-10 soluble receptor, consisting of the extracellular domain of IL-10 R, binds IL-10 with high affinity in solution and is a potent IL-10 antagonist.

目次に戻る

Citations

1. Re-evaluation of IL-10 signaling reveals novel insights on the contribution of the intracellular domain of the IL-10R2 chain
   Authors: RHP Wilbers, DR van Raaij, LB Westerhof, J Bakker, G Smant, A Schots
   PLoS ONE, 2017;12(10):e0186317.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


2. Alternative activation-skewed microglia/macrophages promote hematoma resolution in experimental intracerebral hemorrhage
   Authors: CF Chang, J Wan, Q Li, SC Renfroe, NM Heller, J Wang
   Neurobiol. Dis., 2017;103(0):54-69.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: Functional Assay


3. Mesenchymal Stem Cell-Educated Macrophages Ameliorate LPS-Induced Systemic Response
   Authors: Yaoqin Hu
   Mediators Inflamm, 2016;2016(0):3735452.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


4. IL4I1 Is a Novel Regulator of M2 Macrophage Polarization That Can Inhibit T Cell Activation via L-Tryptophan and Arginine Depletion and IL-10 Production.
   Authors: Yue Y, Huang W, Liang J, Guo J, Ji J, Yao Y, Zheng M, Cai Z, Lu L, Wang J
   PLoS ONE, 2015;10(11):e0142979.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


5. Neuronal synapse formation induced by microglia and interleukin 10.
   Authors: Lim S, Park E, You B, Jung Y, Park A, Park S, Lee J
   PLoS ONE, 2013;8(11):e81218.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


6. IL-10 receptor and coreceptor expression in quiescent and activated hepatic stellate cells.
   Authors: Mathurin P, Xiong S, Kharbanda KK, Veal N, Miyahara T, Motomura K, Rippe RA, Bachem MG, Tsukamoto H
   Am. J. Physiol. Gastrointest. Liver Physiol., 2002;282(6):G981-90.
   Species: Rat
   Sample Type: Whole Cells
   Application: ICC

目次に戻る