抗BLC抗体(Anti-BLC, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse CXCL13/BLC/BCA‑1Ile22-Ala109Accession # Q3U1E8
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse CXCL13/BLC/BCA‑1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CXCL13 and rhGCP-2 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse CXCL13/BLC/BCA‑1 (Catalog # 470-BC)
Immunohistochemistry	5-15 µg/mL	See below
Neutralization	Measured by its ability to neutralize CXCL13/BLC/BCA‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR5. The Neutralization Dose (ND50) is typically 20-50 µg/mL in the presence of 4 µg/mL Recombinant Mouse CXCL13/BLC/BCA‑1.

Neutralization
	Chemotaxis Induced by CXCL13/BLC/BCA‑1 and Neutralization by Mouse CXCL13/BLC/BCA‑1 Antibody. Recombinant Mouse CXCL13/BLC/BCA‑1 (Catalog # 470-BC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin staining. Chemotaxis elicited by Recombinant Mouse CXCL13/BLC/BCA‑1 (4 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL13/BLC/BCA‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF470). The ND50 is typically 20-50 µg/mL.
Immunohistochemistry
	CXCL13/BLC/BCA‑1 in Mouse Intestine. CXCL13/BLC/BCA‑1 was detected in perfusion fixed frozen sections of mouse intestine (Peyer's patch) using Goat Anti-Mouse CXCL13/BLC/BCA‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF470) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to endothelial cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

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Related Product & Information

Entrez Gene IDs	10563 (Human); 55985 (Mouse)
Background	CXCL13/BLC/BCA-1
background_content	Background: CXCL13/BLC/BCA-1 CXCL13, also known as B-lymphocyte chemoattractant (BLC), is a CXC chemokine that is constitutively expressed in secondary lymphoid organs. Mouse BCA-1 cDNA encodes a precursor protein of 109 amino acid residues with a putative leader sequence of 21 residues. Mature mouse BCA-1 shares 64% amino acid sequence similarity with the human protein and 23‑34% amino acid sequence identity with other known CXC chemokines. Recombinant or chemically synthesized BCA-1 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein-coupled receptor originally isolated from Burkitt’s lymphoma cells, has now been shown to be the specific receptor for BCA-1. Among cells of the hematopoietic lineages, the expression of BLR1, now designated CXCR5, is restricted to B lymphocytes and a subpopulation of T helper memory cells. Mice lacking BLR1 have been shown to lack inguinal lymph nodes. These mice were also found to have impaired development of Peyer’s patches and defective formation of primary follicles and germinal centers in the spleen as a result of the inability of B lymphocytes to migrate into B cell areas.

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Citations

1. Application of light sheet microscopy for qualitative and quantitative analysis of bronchus-associated lymphoid tissue in mice
   Authors: DT Mzinza, H Fleige, K Laarmann, S Willenzon, J Ristenpart, J Spanier, G Sutter, U Kalinke, P Valentin-W, R Förster
   Cell. Mol. Immunol., 2018;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC- Fresh Frozen


2. Expression of the B cell differentiation factor BAFF and chemokine CXCL13 in a murine model of Respiratory Syncytial Virus infection
   Authors: W Alturaiki, AJ McFarlane, K Rose, R Corkhill, PS McNamara, J Schwarze, BF Flanagan
   Cytokine, 2018;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC-Fr


3. Ageing adversely affects the migration and function of marginal zone B cells
   Authors: VM Turner, NA Mabbott
   Immunology, 2017;151(3):349-362.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC-Fr


4. Role of SIRP? in Homeostatic Regulation of T Cells and Fibroblastic Reticular Cells in the Spleen
   Authors: D Respatika, Y Saito, K Washio, S Komori, T Kotani, H Okazawa, Y Murata, T Matozaki
   Kobe J Med Sci, 2017;63(1):E22-E29.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC-F


5. Pneumocystis-Driven Inducible Bronchus-Associated Lymphoid Tissue Formation Requires Th2 and Th17 Immunity
   Authors: T Eddens, W Elsegeiny, ML Garcia-Her, P Castillo, G Trevejo-Nu, K Serody, BT Campfield, SA Khader, K Chen, J Rangel-Mor, JK Kolls
   Cell Rep, 2017;18(13):3078-3090.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


6. Brain inflammation is accompanied by peripheral inflammation in Cstb (-/-) mice, a model for progressive myoclonus epilepsy
   J Neuroinflammation, 2016;13(1):298.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC - Frozen


7. Hif1a Deletion Reveals Pro-Neoplastic Function of B Cells in Pancreatic Neoplasia
   Cancer Discov, 2016;6(3):256-69.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: Western Blot


8. Stromal cells as trend-setters for cells migrating into the lymph node.
   Authors: Buettner M, Dittrich-Breiholz O, Falk C, Lochner M, Smoczek A, Menzel F, Bornemann M, Bode U
   Mucosal Immunol, 2015;8(3):640-9.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


9. CLEC-2 is required for development and maintenance of lymph nodes.
   Authors: Benezech C, Nayar S, Finney B, Withers D, Lowe K, Desanti G, Marriott C, Watson S, Caamano J, Buckley C, Barone F
   Blood, 2014;123(20):3200-7.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


10. IL-17-induced CXCL12 recruits B cells and induces follicle formation in BALT in the absence of differentiated FDCs.
    Authors: Fleige H, Ravens S, Moschovakis G, Bolter J, Willenzon S, Sutter G, Haussler S, Kalinke U, Prinz I, Forster R
    J Exp Med, 2014;211(4):643-51.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC

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