抗CXCL10抗体(Anti-CXCL10, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse CXCL10/IP-10/CRG-2Ile22-Pro98Accession # P17515
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse CXCL10/IP-10/CRG-2 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human CXCL10 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Neutralization	Measured by its ability to neutralize CXCL10/IP‑10/CRG‑2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR3. The Neutralization Dose (ND50) is typically 5-25 µg/mL in the presence of 0.5 µg/mL Recombinant Mouse CXCL10/IP‑10/CRG‑2.

Western Blot
	Detection of Mouse CXCL10 by Western Blot. Western blot shows lysates of mouse lymph node tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse CXCL10/IP‑10/CRG‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-466-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CXCL10 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Neutralization
	Chemotaxis Induced by CXCL10/CRG‑2 and Neutral­ization by Mouse CXCL10/CRG‑2 Antibody. Recombinant Mouse CXCL10/CRG‑2 (Catalog # 466‑CR) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL10/CRG‑2 (0.5 µg/mL) is neutral­ized (green line) by increasing concentrations of Goat Anti-Mouse CXCL10/CRG‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-466-NA). The ND50 is typically 5-25 µg/mL.

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Related Product & Information

Entrez Gene IDs	3627 (Human); 15945 (Mouse)
Background	CXCL10/IP-10/CRG-2
background_content	Background: CXCL10/IP-10/CRG-2 The gene for CRG-2, a mouse homolog of human IP-10, was originally identified as an immediate early gene induced in response to macrophage activation. It has since been shown that CRG-2 mRNA is induced by alpha / beta / gamma -interferons and by lipopolysaccharide in macrophages, astrocytes and microglia. Human IP-10 was also shown to be expressed in activated T-lymphocytes, splenocytes, keratinocytes, osteoblasts, astrocytes, and smooth muscle cells. Mouse CRG-2 cDNA encodes a 98 amino acid (aa) residue precursor protein with a 21 aa residue signal peptide that is cleaved to form the 77 aa residue secreted mature protein. Mature CRG-2 shares approximately 67% amino acid sequence identity with human IP-10. The amino acid sequence of CRG-2 identified the protein as a member of the chemokine alpha subfamily that lacks the ELR domain. CRG-2 has been shown to be a chemoattractant for activated T-lymphocytes. Recently, human IP-10 has also been reported to be a potent inhibitor of angiogenesis and to display a potent thymus-dependent anti-tumor effect. A chemokine receptor specific for IP-10 and MIG (CXCR3) has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes.

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Citations

1. Placental growth factor silencing ameliorates liver fibrosis and angiogenesis and inhibits activation of hepatic stellate cells in a murine model of chronic liver disease
   Authors: X Li, QY Yao, HC Liu, QW Jin, BL Xu, SC Zhang, CT Tu
   J. Cell. Mol. Med., 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


2. IFN? Protects Neurons from Damage in a Murine Model of HIV-1 Associated Brain Injury
   Authors: VE Thaney, AM O'Neill, MM Hoefer, R Maung, AB Sanchez, M Kaul
   Sci Rep, 2017;7(0):46514.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


3. SSeCKS/AKAP12 scaffolding functions suppress B16F10-induced peritoneal metastasis by attenuating CXCL9/10 secretion by resident fibroblasts
   Authors: M Muramatsu, L Gao, J Peresie, B Balderman, S Akakura, IH Gelman
   Oncotarget, 2017;8(41):70281-70298.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


4. CXCR3 Blockade Inhibits T Cell Migration into the Skin and Prevents Development of Alopecia Areata
   J Immunol, 2016;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


5. Brain inflammation is accompanied by peripheral inflammation in Cstb (-/-) mice, a model for progressive myoclonus epilepsy
   J Neuroinflammation, 2016;13(1):298.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC - Frozen


6. Deletion of astroglial CXCL10 delays clinical onset but does not affect progressive axon loss in a murine autoimmune multiple sclerosis model.
   Authors: Mills Ko E, Ma J, Guo F, Miers L, Lee E, Bannerman P, Burns T, Ko D, Sohn J, Soulika A, Pleasure D
   J Neuroinflammation, 2014;11(0):105.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


7. Divergent effects of T cell costimulation and inflammatory cytokine production on autoimmune peripheral neuropathy provoked by Aire deficiency.
   Authors: Zeng X, Nagavalli A, Smith C, Howard J, Su M
   J Immunol, 2013;190(8):3895-904.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC OCT-embedded


8. Nonviral delivery of small interfering RNA into pancreas-associated immune cells prevents autoimmune diabetes.
   Authors: Leconet W, Petit P, Peraldi-Roux S, Bresson D
   Mol Ther, 2012;20(12):2315-25.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


9. Comprehensive assessment of chemokine expression profiles by flow cytometry.
   Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
   J. Clin. Invest., 2010;120(3):907-23.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


10. Interferon-gamma and the interferon-inducible chemokine CXCL10 protect against aneurysm formation and rupture.
    Authors: King VL, Lin AY, Kristo F, Anderson TJ, Ahluwalia N, Hardy GJ, Owens AP, Howatt DA, Shen D, Tager AM, Luster AD, Daugherty A, Gerszten RE
    Circulation, 2009;119(3):426-35.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Fresh

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