抗IL-17 Receptor抗体(Anti-IL-17 Receptor, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

目次に戻る

Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL‑17 RA/IL‑17 RExtracellular domain
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse IL‑17 RA/IL‑17 R in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human IL‑17 RA/IL‑17 R is observed and less than 1% cross-reactivity with recombinant mouse (rm) IL-17 RC and rmIL-17 RD is observed.

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Flow Cytometry	2.5 µg/106 cells	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Western Blot
	Detection of Mouse IL‑17 RA/IL‑17 R by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse IL‑17 RA/IL‑17 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF448) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL‑17 RA/IL‑17 R at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Flow Cytometry
	Detection of IL‑17 RA/IL‑17 R in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macro­phage cell line was stained with Goat Anti-Mouse IL‑17 RA/IL‑17 R Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF448, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

目次に戻る

Related Product & Information

Background

IL-17 RA/IL-17 R

background_content

Background: IL-17 RA/IL-17 R IL-17 R, also known as IL-17 RA, is a 120 kDa type I transmembrane glycoprotein protein that plays a central role in inflammatory responses (1-3). Mature mouseIL‑17 R consists of a 291 amino acid (aa) extracellular domain, a 21 aa transmembrane segment, and a 521 aa cytoplasmic domain (4). The cytoplasmic domain contains a region homologous to the TIR domain of the TLR/IL-1 R family (5). Mouse IL-17 R shares 84% and 72% aa sequence identity with rat and human IL-17 R, respectively. Within the extracellular domain, it shares 18-25% sequence identity with mouse IL-17 RB, C, D, and E. While the expression of IL-17 is restricted to activated T cells, IL-17 R exhibits a broad tissue distribution (4). Even in the absence of ligand, IL-17 R exists on the cell surface as a multimer (6). IL-17 R can bind IL-17 but must associate with IL-17 RC to transduce signals (7, 8). Interestingly, human IL-17 R does not appear to form productive complexes with mouse IL-17 RC (8). The IL-17 R can also signal in response to IL-17F (9). IL-17 R ligation promotes T cell activation and the production of IL-6, G-CSF, SCF, and multiple pro‑inflammatory chemokines (4, 7, 9, 10). IL-17A and IL-17F synergize with TNF-alpha in the induction of CXCL1, G-CSF, and IL-6 (9, 11). This effect requires the presence of both TNF RI and TNF RII (9). IL-17 interactions with IL-17 R also inhibit the TNF-alpha induced upregulation of fibroblast CCL5 and VCAM-1 (11). CCL5 and VCAM-1 induced effects are differentially sensitive to blockade with IL-17 R specific antibodies, suggesting that IL-17 R triggers divergent intracellular signals (11). In vivo, IL‑17 R activity is important for increased generation of neutrophils and their recruitment to sites of inflammation (10, 12, 13). IL-17 R is required for host defense against microbial infection and for the progression of arthritis from inflammation to destructive joint erosion (10, 13).

目次に戻る

Citations

1. Population snapshots predict early haematopoietic and erythroid hierarchies
   Authors: BK Tusi, SL Wolock, C Weinreb, Y Hwang, D Hidalgo, R Zilionis, A Waisman, JR Huh, AM Klein, M Socolovsky
   Nature, 2018;0(0):.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


2. IL-17 Augments B Cell Activation in Ocular Surface Autoimmunity
   J Immunol, 2016;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


3. TLR2 deficiency leads to increased Th17 infiltrates in experimental brain abscesses.
   Authors: Nichols JR, Aldrich AL, Mariani MM, Vidlak D, Esen N, Kielian T
   J. Immunol., 2009;182(11):7119-30.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


4. IFN-alpha enhances peptide vaccine-induced CD8+ T cell numbers, effector function, and antitumor activity.
   Authors: Sikora AG, Jaffarzad N, Hailemichael Y, Gelbard A, Stonier SW, Schluns KS, Frasca L, Lou Y, Liu C, Andersson HA, Hwu P, Overwijk WW
   J. Immunol., 2009;182(12):7398-407.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


5. IL-17RD (Sef or IL-17RLM) interacts with IL-17 receptor and mediates IL-17 signaling.
   Authors: Rong Z, Wang A, Li Z, Ren Y, Cheng L, Li Y, Wang Y, Ren F, Zhang X, Hu J, Chang Z
   Cell Res., 2009;19(2):208-15.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: IP


6. IL-17 receptor signaling influences virus-induced corneal inflammation.
   Authors: Molesworth-Kenyon SJ, Yin R, Oakes JE, Lausch RN
   J. Leukoc. Biol., 2007;83(2):401-8.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


7. Inhibition of interleukin-17 prevents the development of arthritis in vaccinated mice challenged with Borrelia burgdorferi.
   Authors: Burchill MA, Nardelli DT, England DM, DeCoster DJ, Christopherson JA, Callister SM, Schell RF
   Infect. Immun., 2003;71(6):3437-42.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


8. Functional interleukin-17 receptor A is expressed in central nervous system glia and upregulated in experimental autoimmune encephalomyelitis.
   Authors: Das Sarma J, Ciric B, Marek R, Sadhukhan S, Caruso M, Shafagh J, Fitzgerald D, Shindler K, Rostami A
   J Neuroinflammation, 0;6(0):14.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow

目次に戻る