抗FABP4抗体(Anti-FABP4, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse, Rat
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse FABP4Met1-Ala132Accession # P04117
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse FABP4 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse (rm) FABP5 and rmFABP9 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.25 µg/mL	See below
Simple Western	2.5 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below
Immunocytochemistry	5-15 µg/mL	See below

Western Blot
	Detection of Mouse FABP4/A‑FABP by Western Blot. Western blot shows lysates of mouse thymus tissue and mouse heart tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse/Rat FABP4/A‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected forFABP4/A‑FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
	FABP4 in Mouse Adipocytes. FABP4 was detected in immersion fixed mouse mesenchymal stem cell-derived adipocytes using 10 µg/mL Goat Anti-Mouse/Rat FABP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
	FABP4 in Rat Mesenchymal Stem Cells. FABP4 was detected in immersion fixed rat mesenchymal stem cells differentiated to adipocytes using Goat Anti-Mouse/Rat FABP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
	FABP4 in Mouse Intestine. FABP4 was detected in perfusion fixed frozen sections of mouse intestine using Goat Anti-Mouse/Rat FABP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of epithelial cells in intestinal glands. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Simple Western
	Detection of Mouse FABP4/A‑FABP by Simple WesternTM. Simple Western lane view shows lysates of mouse thymus tissue and mouse heart tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP4/A‑FABP at approximately 17 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse/Rat FABP4/A‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Related Product & Information

Long Name	Fatty Acid-Binding Protein 4
Background	FABP4/A-FABP
background_content	Background: FABP4/A-FABP FABP-4 (fatty acid binding protein-4; also Adipocyte-type FABP, ALBP and aP2) is a 14-15 kDa member of the fatty acid binding protein family, calycin superfamily of molecules.  It is expressed by adipocytes, macrophages, monocyte-derived DC and myoepithelial cells, and is known to serve as a carrier for select PPAR gamma fatty acid ligands as they enter the nucleus.  These ligands, or fatty acids, facilitate PPAR gamma -mediated gene transcription.  Mouse FABP-4 is 132 amino acids (aa) in length. It is a two beta -sheet molecule that contains an NLS (aa 22-32) and a fatty acid binding segment (aa 127-129).  Mouse FABP-4 is 94% and 92% aa identical to rat and human FABP-4, respectively.

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Citations

1. Chemotherapy can induce weight normalization of morbidly obese mice despite undiminished ingestion of high fat diet
   Authors: CE Myers, DB Hoelzinger, TN Truong, LA Chew, A Myles, L Chaudhuri, JB Egan, J Liu, SJ Gendler, PA Cohen
   Oncotarget, 2017;8(3):5426-5438.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


2. A-FABP mediates adaptive thermogenesis by promoting intracellular activation of thyroid hormones in brown adipocytes
   Authors: L Shu, RL Hoo, X Wu, Y Pan, IP Lee, LY Cheong, SR Bornstein, X Rong, J Guo, A Xu
   Nat Commun, 2017;8(0):14147.
   Species: Mouse
   Sample Type: Serum
   Application: IP


3. Epigenetic programming of Dnmt3a mediated by AP2? is required for granting preadipocyte the ability to differentiate
   Cell Death Dis, 2016;7(12):e2496.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


4. Epigenetic programming of Dnmt3a mediated by AP2? is required for granting preadipocyte the ability to differentiate
   Cell Death Dis, 2016;7(12):e2496.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


5. Epidermal Wnt/beta-catenin signaling regulates adipocyte differentiation via secretion of adipogenic factors.
   Authors: Donati G, Proserpio V, Lichtenberger B, Natsuga K, Sinclair R, Fujiwara H, Watt F
   Proc Natl Acad Sci U S A, 2014;111(15):E1501-9.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


6. Artificial sweeteners stimulate adipogenesis and suppress lipolysis independently of sweet taste receptors.
   Authors: Simon B, Parlee S, Learman B, Mori H, Scheller E, Cawthorn W, Ning X, Gallagher K, Tyrberg B, Assadi-Porter F, Evans C, MacDougald O
   J Biol Chem, 2013;288(45):32475-89.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


7. Distinct fibroblast lineages determine dermal architecture in skin development and repair.
   Authors: Driskell R, Lichtenberger B, Hoste E, Kretzschmar K, Simons B, Charalambous M, Ferron S, Herault Y, Pavlovic G, Ferguson-Smith A, Watt F
   Nature, 2013;504(7479):277-81.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


8. Cyclooxygenase-2 deficiency attenuates adipose tissue differentiation and inflammation in mice.
   Authors: Ghoshal S, Trivedi DB, Graf GA, Loftin CD
   J. Biol. Chem., 2011;286(1):889-98.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


9. Age-related changes in rat bone-marrow mesenchymal stem cell plasticity.
   Authors: Asumda FZ, Chase PB
   BMC Cell Biol., 2011;12(0):44.
   Species: Rat
   Sample Type: Whole Cells
   Application: ICC

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