抗MDC抗体(Anti-MDC, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse CCL22/MDCGly25-Ser92Accession # Q9QZU2
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse CCL22/MDC in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human CCL22/MDC is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse CCL22/MDC (Catalog # 439-MD)
Neutralization	Measured by its ability to neutralize CCL22/MDC-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR4. The Neutralization Dose (ND50) is typically 0.3-1.0 µg/mL in the presence of 15 ng/mL Recombinant Mouse CCL22/MDC.

Neutralization

Chemotaxis Induced by CCL22/MDC and Neutralization by Mouse CCL22/MDC Antibody. Recombinant Mouse CCL22/MDC (Catalog # 439‑MD) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Mouse CCL22/MDC (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL22/MDC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF439). The ND50 is typically 0.3-1.0 µg/mL.

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Related Product & Information

Entrez Gene IDs	6367 (Human); 20299 (Mouse)
Background	CCL22/MDC
background_content	Background: CCL22/MDC Mouse ABCD-1, the mouse orthologue of the human macrophage-derived chemokine (MDC)/stimulated T cell chemotactic protein (STCP-1), is a CC chemokine cloned from activated mouse B cells. Mouse MDC cDNA encodes a precursor protein of 92 amino acid (aa) residues with a 24 aa residue predicted signal peptide that is cleaved to yield a 68 aa residue mature 7.8 kDa protein. At the amino acid sequence level, mouse and human MDC share 64% identity and 83% similarity. The genomic organization of the mouse and human MDC genes are very similar, exhibiting sequence identity at the intron-exon boundaries. Mouse MDC is expressed at high levels in dendritic cells and activated B lymphocytes. Low levels of mouse MDC mRNA are also detectable in lung, unstimulated spleen cells, lymph node cells and in thymocytes. MDC is a functional ligand for the CC chemokine receptor 4. Recombinant or chemically synthesized mature mouse MDC has been shown to induce chemotaxis or Ca2+ mobilization in activated mouse and human T cells.

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Citations

1. IL-4/CCL22/CCR4 axis controls regulatory T-cell migration that suppresses inflammatory bone loss in murine experimental periodontitis.
   Authors: Araujo-Pires A, Vieira A, Francisconi C, Biguetti C, Glowacki A, Yoshizawa S, Campanelli A, Trombone A, Sfeir C, Little S, Garlet G
   J Bone Miner Res, 2015;30(3):412-22.
   Species: Mouse
   Sample Type: In Vivo
   Application: Neut


2. Defective immunoregulation in RSV vaccine-augmented viral lung disease restored by selective chemoattraction of regulatory T cells.
   Authors: Loebbermann, Jens, Durant, Lydia, Thornton, Hannah, Johansson, Cecilia, Openshaw, Peter J
   Proc Natl Acad Sci U S A, 2013;110(8):2987-92.
   Species: Mouse
   Sample Type: In Vivo
   Application: Neut


3. Prevention of murine autoimmune diabetes by CCL22-mediated Treg recruitment to the pancreatic islets.
   Authors: Montane J, Bischoff L, Soukhatcheva G, Dai DL, Hardenberg G, Levings MK, Orban PC, Kieffer TJ, Tan R, Verchere CB
   J. Clin. Invest., 2011;121(0):3024.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


4. Comprehensive assessment of chemokine expression profiles by flow cytometry.
   Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
   J. Clin. Invest., 2010;120(3):907-23.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


5. Allergic pulmonary inflammation in mice is dependent on eosinophil-induced recruitment of effector T cells.
   Authors: Jacobsen EA, Ochkur SI, Pero RS, Taranova AG, Protheroe CA, Colbert DC, Lee NA, Lee JJ
   J. Exp. Med., 2008;205(3):699-710.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


6. The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells.
   Authors: Fujita H, Asahina A, Komine M, Tamaki K
   Cell. Immunol., 2007;245(2):70-9.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: Functional Assay


7. Immunosuppressive effects of CCL17 on pulmonary antifungal responses during pulmonary invasive aspergillosis.
   Authors: Carpenter KJ, Hogaboam CM
   Infect. Immun., 2005;73(11):7198-207.
   Species: Mouse
   Sample Type:
   Application: Neut

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