抗GFRα2抗体(Anti-GFRα2, Human/Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse GFR alpha ‑2/GDNF R alpha ‑2Ser22-Ser441Accession # Q3UUD8
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human and mouse GFR alpha ‑2/GDNF R alpha ‑2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than5% cross‑reactivity with recombinant human GFR alpha -3 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse GFR alpha ‑2/GDNF R alpha ‑2 Fc Chimera (Catalog # 429-FR)
Immunohistochemistry	5-15 µg/mL	See below
Blockade of Receptor-ligand Interaction	In a functional ELISA, 1-3 µg/mL of this antibody will block 50% of the binding of 2 ng/mL of Recombinant Human GDNF (Catalog # 212-GD) to immobilized Recombinant Mouse GFR alpha -2 Fc Chimera (Catalog # 429-FR) coated at 1 µg/mL (100 µL/well). At 15 μg/mL, this antibody will block >90% of the binding.

Immunohistochemistry

GFR alpha ‑2/GDNF R alpha ‑2 in Mouse Dorsal Root Ganglion. GFR alpha ‑2/GDNF R alpha ‑2 was detected in perfusion fixed frozen sections of mouse dorsal root ganglion using Goat Anti-Human/Mouse GFR alpha ‑2/GDNF R alpha ‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF429) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane of sensory neurons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

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Related Product & Information

Long Name	Glial Cell line-derived Neurotrophic Factor Receptor alpha 2
Entrez Gene IDs	2675 (Human); 14586 (Mouse)
Background	GFR alpha-2/GDNF R alpha-2
background_content	Background: GFR alpha-2/GDNF R alpha-2 Glial cell line-derived growth factor (GDNF), neurturin (NTN), persephin (PSP) and artemin, distant members of the TGF-beta  superfamily, are neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. The bioactivities of GDNF and NTN are mediated through a receptor complex composed of the non ligand-binding signaling subunit (c-Ret receptor tyrosine kinase) and either of two ligand binding subunits, GDNF receptor alpha -1 (GFR alpha -1, also known as Trn R1) or GFR alpha -2 (also known as Trn R2). GFR alpha -1 and -2 are members of a family of at least four cysteine-rich glycosyl-phosphatidylinositol (GPI)-linked cell surface proteins that share conserved placements of many of their cysteine residues. Binding of GDNF or NTN to membrane-associated GFR alpha -1 or GFR alpha -2 initiates the association with and activation of the Ret tyrosine kinase. Mouse GFR alpha -2 cDNA encodes a 463 amino acid (aa) residue protein with a putative N-terminal 21 aa residue hydrophobic signal peptide. Like other GPI-linked proteins, rat GFR alpha -2 has a C-terminal hydrophobic region which is preceded by a 3 aa residue (SGS) GPI-binding site. Human GFR alpha -2 shares 96.5% amino acid identity with mouse GFR alpha -2. The expression of the various GFR alpha s are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alpha s in mediating the activities of the GDNF family of neurotrophic factors.

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Citations

1. Protection of nigral dopaminergic neurons by AAV1 transduction with Rheb(S16H) against neurotoxic inflammation in vivo
   Authors: S Kim, GJ Moon, YS Oh, J Park, WH Shin, JY Jeong, KS Choi, BK Jin, N Kholodilov, RE Burke, HJ Kim, CM Ha, SG Lee, SR Kim
   Exp. Mol. Med., 2018;50(2):e440.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


2. Salivary glands regenerate after radiation injury through SOX2-mediated secretory cell replacement
   Authors: E Emmerson, AJ May, L Berthoin, N Cruz-Pache, S Nathan, AJ Mattingly, JL Chang, WR Ryan, AD Tward, SM Knox
   EMBO Mol Med, 2018;0(0):.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC - Paraffin embedded


3. Neurturin Gene Therapy Protects Parasympathetic Function to Prevent Irradiation-Induced Murine Salivary Gland Hypofunction
   Authors: JNA Ferreira, C Zheng, IMA Lombaert, CM Goldsmith, AP Cotrim, JM Symonds, VN Patel, MP Hoffman
   Mol Ther Methods Clin Dev, 2018;9(0):172-180.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


4. Neurturin and a Glp-1 Analogue Act Synergistically to Alleviate Diabetes in Zucker Diabetic Fatty Rats
   Authors: JL Trevaskis, CB Sacramento, H Jouihan, S Ali, J Le Lay, S Oldham, N Bhagroo, BB Boland, J Cann, Y Chang, T O'Day, V Howard, C Reers, MS Winzell, DM Smith, M Feigh, P Barkholt, K Schreiter, M Austen, U Andag, S Thompson, L Jermutus, MP Coghlan, J Grimsby, C Dohrmann, CJ Rhodes, CM Rondinone, A Sharma
   Diabetes, 2017;0(0):.
   Species: Rat
   Sample Type: Whole Tissue
   Application: IHC


5. Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing.
   Authors: Usoskin D, Furlan A, Islam S, Abdo H, Lonnerberg P, Lou D, Hjerling-Leffler J, Haeggstrom J, Kharchenko O, Kharchenko P, Linnarsson S, Ernfors P
   Nat Neurosci, 2015;18(1):145-53.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


6. Parasympathetic stimulation improves epithelial organ regeneration.
   Authors: Knox, Sarah M, Lombaert, Isabelle, Haddox, Candace, Abrams, Shaun R, Cotrim, Ana, Wilson, Adrian J, Hoffman, Matthew
   Nat Commun, 2013;4(0):1494.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


7. Intracerebral infusion of glial cell line-derived neurotrophic factor promotes striatal neurogenesis after stroke in adult rats.
   Authors: Kobayashi T, Ahlenius H, Thored P, Kobayashi R, Kokaia Z, Lindvall O
   Stroke, 2006;37(9):2361-7.
   Species: Rat
   Sample Type: Whole Cells
   Application: ICC


8. Glial cell line-derived neurotrophic factor family members sensitize nociceptors in vitro and produce thermal hyperalgesia in vivo.
   Authors: Malin SA, Molliver DC, Koerber HR, Cornuet P, Frye R, Albers KM, Davis BM
   J. Neurosci., 2006;26(33):8588-99.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


9. Neurotrophin and GDNF family ligands promote survival and alter excitotoxic vulnerability of neurons derived from murine embryonic stem cells.
   Authors: Lee CS, Tee LY, Dusenbery S, Takata T, Golden JP, Pierchala BA, Gottlieb DI, Johnson EM, Choi DW, Snider BJ
   Exp. Neurol., 2005;191(1):65-76.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


10. Expression and function of GDNF family ligands and receptors in the carotid body.
    Authors: Leitner ML, Wang LH, Osborne PA, Golden JP, Milbrandt J, Johnson EM
    Exp. Neurol., 2005;191(0):S68-79.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC

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