抗IL-12抗体(Anti-IL-12, Mouse, Goat-Poly antibody)

掲載日情報:2019/10/21 現在Webページ番号:26993

IL-12に対する抗体(Anti-IL-12, Mouse, Goat-Poly )です。
本製品は研究用です。研究用以外には使用できません。

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[在庫・価格 :2025年04月26日 20時55分現在]

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Anti-IL-12, Mouse, Goat-Poly
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 2
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説明文
別名:CLMF p35
Genbank No: 3592
法規制等
保存条件 -20℃ 法規備考
抗原種 Mouse 免疫動物 Goat クラス IgG 標識 Unlabeled
交差性 Mouse 適用 Neutralising,Western Blot
クロナリティ Polyclonal フォーマット 性状 Protein A/G Affinity Purified 吸収処理
掲載カタログ

製品記事 M1/M2 Macrophage Activation Marker
Helper T Cells Marker Antibodies
関連記事

[在庫・価格 :2025年04月26日 20時55分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-IL-12, Mouse, Goat-Poly

文献数: 2

説明文 別名:CLMF p35
Genbank No: 3592
法規制等
保存条件 -20℃ 法規備考
抗原種 Mouse 免疫動物 Goat
交差性 Mouse 適用 Neutralising,Western Blot
標識 Unlabeled 性状 Protein A/G Affinity Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 M1/M2 Macrophage Activation Marker
Helper T Cells Marker Antibodies
関連記事



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Product Details

Species ReactivityMouse
LabelUnconjugated
ImmunogenS. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL‑12
SourcePolyclonal Goat IgG
PurificationProtein A or G purified
SpecificityDetects mouse IL-12 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human IL‑12 and less than 5% cross-reactivity with recombinant porcine IL-12 is observed.


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Applications and Data

 Recommended
Concentration
Sample
Western Blot1 µg/mLRecombinant Mouse IL‑12 (Catalog # 419-ML)
NeutralizationMeasured by its ability to neutralize IL‑12-induced proliferation in PHA-activated mouse splenocytes [Mattner, F. et al. (1993) Eur. J. Immunol. 23:2202]. The Neutralization Dose (ND50) is typically 0.2-0.5 µg/mL in the presence of 0.3 ng/mL Recombinant Mouse IL‑12.


Neutralization
Cell Proliferation Induced by IL‑12 and Neutralization by Mouse IL‑12 Antibody.
Recombinant Mouse IL‑12 (Catalog # 419-ML) stimulates proliferation in PHA-activated mouse splenocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑12 (0.3 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑12 Polyclonal Antibody (Catalog # AB-419-NA). The ND50 is typically 0.2‑0.5 µg/mL.


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Related Product & Information

Long NameInterleukin 12
Entrez Gene IDs3592 (Human); 16159 (Mouse); 84405 (Rat); 403977 (Canine); 493741 (Feline)
BackgroundIL-12
background_contentBackground:
IL-12
Interleukin 12, also known as Natural Killer Cell Stimulatory Factor (NKSF) or Cytotoxic Lymphocyte Maturation Factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. IL‑12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and Natural Killer (NK) cells. Some of these IL‑12 activities include the induction of IFN‑ gamma and TNF in resting and activated T and NK cells; the enhancement of cytotoxic activity of resting NK and T cells, the stimulation of resting T cell proliferation in the presence of a comitogen; and the enhancement of NK cell proliferation. Current evidence indicates that IL‑12 is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. Based on its activities, it has been suggested that IL‑12 may have therapeutic potential as a vaccine adjuvant that promotes cellular-immunity and as an anti‑tumor and anti‑viral agent. Human and mouse IL‑12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively. While mouse IL‑12 is active on both human and mouse cells, human IL‑12 is not active on murine cells. R&D Systems' recombinant mouse IL‑12 preparations were proteolytically cleaved between residues G158 and E159 of the mature p35 subunit. Thus, under reducing conditions, three bands representing the p40 subunit, the p35 R1‑G158 peptide and the p35 E159‑A193 peptide can be observed in SDS-PAGE. The biological activity of this cleaved mouse IL‑12 is comparable to that of the intact human IL‑12.


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Citations

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
  1. LeIF: a recombinant Leishmania protein that induces an IL-12-mediated Th1 cytokine profile.
    Authors: Skeiky YA, Kennedy M, Kaufman D
    J. Immunol., 1998;161(11):6171-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut



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