抗M-CSF抗体(Anti-M-CSF, Mouse, Goat-Poly antibody)

掲載日情報:2019/10/21 現在Webページ番号:26990

M-CSFに対する抗体(Anti-M-CSF, Mouse, Goat-Poly )です。
本製品は研究用です。研究用以外には使用できません。

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納期 文献数
Anti-M-CSF, Mouse, Goat-Poly
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 1
説明文
別名:colony stimulating factor 1 (macrophage)
Genbank No: 1435
Protein Accession No: Q3U4F9
法規制等
保存条件 -20℃ 法規備考
抗原種 Mouse 免疫動物 Goat クラス IgG 標識 Unlabeled
交差性 Mouse 適用 Neutralising,Western Blot
クロナリティ Polyclonal フォーマット 性状 Protein A/G Affinity Purified 吸収処理
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製品記事 抗幹細胞マーカー抗体/抗造血幹細胞マーカー抗体(R&D Systems社)
関連記事

[在庫・価格 :2024年05月01日 00時01分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-M-CSF, Mouse, Goat-Poly

文献数: 1

説明文 別名:colony stimulating factor 1 (macrophage)
Genbank No: 1435
Protein Accession No: Q3U4F9
法規制等
保存条件 -20℃ 法規備考
抗原種 Mouse 免疫動物 Goat
交差性 Mouse 適用 Neutralising,Western Blot
標識 Unlabeled 性状 Protein A/G Affinity Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 抗幹細胞マーカー抗体/抗造血幹細胞マーカー抗体(R&D Systems社)
関連記事



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Product Details

Species ReactivityMouse
LabelUnconjugated
ImmunogenE. coli-derived recombinant mouse M-CSFLys33-Glu262Accession # Q3U4F9
SourcePolyclonal Goat IgG
PurificationProtein A or G purified
SpecificityDetects mouse M‑CSF in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) M‑CSF is observed. Neutralizes the biological activity of recombinant mouse M-CSF and will also neutralize the biological activity of recombinant human M-CSF at a 100 fold higher IgG concentration.


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Applications and Data

 Recommended
Concentration
Sample
Western Blot1 µg/mLRecombinant Mouse M-CSF (Catalog # 416-ML)
NeutralizationMeasured by its ability to neutralize M‑CSF-induced proliferation in the M‑NFS‑60 mouse myelogenous leukemia lymphoblast cell line. Halenbeck, R. et al. (1989) Biotechnology 7:710. The Neutralization Dose (ND50) is typically 0.2-0.6 µg/mL in the presence of 10 ng/mL Recombinant Mouse M‑CSF.


Neutralization
Cell Proliferation Induced by M‑CSF and Neutralization by Mouse M‑CSF Antibody.
Recombinant Mouse M‑CSF (Catalog # 416-ML) stimulates proliferation in the M‑NFS‑60 mouse myelogenous leukemia lymphoblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse M‑CSF (10 ng/mL) is neutralized (green line) by increasing concen­trations of Goat Anti-Mouse M‑CSF Polyclonal Antibody (Catalog # AB-416-NA). The ND50 is typically 0.2‑0.6 µg/mL.


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Related Product & Information

Long NameMacrophage Colony Stimulating Factor
Entrez Gene IDs1435 (Human); 12977 (Mouse)
BackgroundM-CSF
background_contentBackground:
M-CSF
M-CSF, also known as CSF-1, is a four-alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation (1‑3). M-CSF is also essential for the survival and proliferation of osteoclast progenitors (1, 4). M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis (2, 3). M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta (5). Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells (1‑5). The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur (3‑9). Full length mouse M-CSF transcripts encode a 520 amino acid (aa) type I transmembrane (TM) protein with a 462 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O- glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF can circulate, it may be immobilized by attachment to type V collagen (8). Shorter transcripts encode M‑CSF that lacks cleavage and PG sites and produces an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer (7). Although forms may vary in activity and half-life, all contain the N-terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor (10, 11). The first 229 aa of mature mouse M-CSF shares 87%, 83%, 82% and 81% aa identity with corresponding regions of rat, dog, cow and human M-CSF, respectively (12, 13). Human M‑CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.


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Citations

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
  1. IL-34 is a Treg-specific cytokine and mediates transplant tolerance.
    Authors: Bezie S, Picarda E, Ossart J, Tesson L, Usal C, Renaudin K, Anegon I, Guillonneau C
    J Clin Invest, 2015;125(10):3952-64.



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