抗GM-CSF抗体(Anti-GM-CSF, Mouse, Goat-Poly antibody)

掲載日情報:2019/10/21 現在Webページ番号:26989

GM-CSFに対する抗体(Anti-GM-CSF, Mouse, Goat-Poly )です。
本製品は研究用です。研究用以外には使用できません。

価格

[在庫・価格 :2024年05月17日 18時55分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
詳細 商品名
  • 商品コード
  • メーカー
  • 包装
  • 価格
  • 在庫
  • 法規制等
納期 文献数
Anti-GM-CSF, Mouse, Goat-Poly
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 1
説明文
別名:colony stimulating factor 2 (granulocyte-macrophage)
Genbank No: 1437
Protein Accession No: Q14AD9
法規制等
保存条件 -20℃ 法規備考
抗原種 Mouse 免疫動物 Goat クラス IgG 標識 Unlabeled
交差性 Mouse 適用 Neutralising,Western Blot
クロナリティ Polyclonal フォーマット 性状 Protein A/G Affinity Purified 吸収処理
掲載カタログ

製品記事 M1/M2 Macrophage Activation Marker
抗幹細胞マーカー抗体/抗造血幹細胞マーカー抗体(R&D Systems社)
関連記事

[在庫・価格 :2024年05月17日 18時55分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-GM-CSF, Mouse, Goat-Poly

文献数: 1

説明文 別名:colony stimulating factor 2 (granulocyte-macrophage)
Genbank No: 1437
Protein Accession No: Q14AD9
法規制等
保存条件 -20℃ 法規備考
抗原種 Mouse 免疫動物 Goat
交差性 Mouse 適用 Neutralising,Western Blot
標識 Unlabeled 性状 Protein A/G Affinity Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 M1/M2 Macrophage Activation Marker
抗幹細胞マーカー抗体/抗造血幹細胞マーカー抗体(R&D Systems社)
関連記事



目次に戻る

Product Details

Species ReactivityMouse
LabelUnconjugated
ImmunogenE. coli-derived recombinant mouse GM-CSFAla18-Lys141Accession # Q14AD9
SourcePolyclonal Goat IgG
PurificationProtein A or G purified
SpecificityDetects mouse GM‑CSF in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human GM‑CSF and recombinant rat GM‑CSF is observed, and less than 3% cross-reactivity with recombinant feline GM‑CSF, recombinant porcine GM‑CSF, and recombinant canine GM‑CSF is observed.


目次に戻る

Applications and Data

 Recommended
Concentration
Sample
Western Blot1 µg/mLRecombinant Mouse GM-CSF (Catalog # 415-ML)
NeutralizationMeasured by its ability to neutralize GM‑CSF-induced proliferation in the DA3 mouse myeloma cell line. Ihle, J. N. et al. (1984) Advances in Viral Oncology. In G. Klein (eds): Raven Press, New York, NY. 4:95. The Neutralization Dose (ND50) is typically 0.01-0.05 µg/mL in the presence of 0.1 ng/mL Recombinant Mouse GM‑CSF.


Neutralization
Cell Proliferation Induced by GM‑CSF and Neutralization by Mouse GM‑CSF Antibody.
Recombinant Mouse GM‑CSF (Catalog # 415-ML) stimulates proliferation in the DA3 mouse myeloma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse GM‑CSF (0.1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse GM‑CSF Polyclonal Antibody (Catalog # AB-415-NA). The ND50 is typically 0.01‑0.05 µg/mL.


目次に戻る

Related Product & Information

Long NameGranulocyte Macrophage Growth Factor
Entrez Gene IDs1437 (Human); 12981 (Mouse); 116630 (Rat); 397208 (Porcine); 403923 (Canine); 493805 (Feline)
BackgroundGM-CSF
background_contentBackground:
GM-CSF
GM-CSF was initially characterized as a factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF is produced by a number of different cell types (including T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes) in response to cytokine or inflammatory stimuli. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages, and eosinophils. GM-CSF promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity. It shows clinical effectiveness in ameliorating chemotherapy-induced neutropenia, and GM-CSF transfected tumor cells are utilized as cancer vaccines. The 22 kDa glycosylated GM-CSF, similar to IL-3 and IL-5, is a cytokine with a core of four bundled alpha ‑helices. Mature mouse GM-CSF shares 49-54% amino acid sequence identity with canine, feline, human, and porcine GM-CSF and 69% with rat GM-CSF. GM‑CSF exerts its biological effects through a heterodimeric receptor complex composed of GM-CSF R alpha /CD116 and the signal transducing common beta chain (CD131) which is also a component of the high-affinity receptors for IL-3 and IL-5. In addition, GM-CSF binds a naturally occurring soluble form of GM-CSF R alpha. The activity of GM-CSF is species specific between human and mouse. Mouse GM-CSF is only weakly active on rat cells, although rat GM-CSF is fully active on mouse cells.


目次に戻る

Citations

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
  1. Induction of differentiation in a B lymphoma X B lymphocyte hybrid line. II. Intraclonal heterogeneity in growth, secretion of IgM, and cytokine production in response to lipopolysaccharide.
    Authors: Little J, Alling DW, Asofsky R
    J. Immunol., 1993;150(6):2129-38.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut



目次に戻る

お問い合わせ先

(テクニカルサポート 試薬担当)

reagent@funakoshi.co.jp

製品情報は掲載時点のものですが、価格表内の価格については随時最新のものに更新されます。お問い合わせいただくタイミングにより製品情報・価格などは変更されている場合があります。
表示価格に、消費税等は含まれていません。一部価格が予告なく変更される場合がありますので、あらかじめご了承下さい。