抗G-CSF抗体(Anti-G-CSF, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse G-CSFVal31-Ala208Accession # P09920
Source	Polyclonal Goat IgG
Purification	Protein A or G purified
Specificity	Detects mouse G-CSF in direct ELISAs and Western blots.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Mouse G-CSF (Catalog # 414-CS)
Neutralization	Measured by its ability to neutralize G‑CSF-induced proliferation in the NFS-60 mouse myeloid cell line. The Neutralization Dose (ND50) is typically <1 µg/mL in the presence of 0.125 ng/mL Recombinant Mouse G‑CSF.

Neutralization

Cell Proliferation Induced by G‑CSF and Neutralization by Goat Anti-Mouse G‑CSF Antibody. Recombinant Mouse G‑CSF (Catalog # 414-CS) stimulates proliferation in the NFS-60 mouse myeloid cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse G‑CSF (0.125 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse G‑CSF Polyclonal Antibody (Catalog # AB-414-NA). The ND50 is typically 1 µg/mL.

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Related Product & Information

Long Name	Granulocyte Colony Stimulating Factor
Entrez Gene IDs	1440 (Human); 12985 (Mouse)
Background	G-CSF
background_content	Background: G-CSF G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete G-CSF after LPS, IL-1 or TNF-alpha activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively. The murine G-CSF cDNA encodes a 208 amino acid (aa) residue precursor protein containing a 30 aa residue signal peptide that is proteolytically cleaved to generate the 178 aa residue mature protein. Human G-CSF is 73% identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity. In vitro, G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage. It also has blast cell growth factor activity and can synergize with IL-3 to shorten the Go period of early hematopoietic progenitors. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis. Recombinant human G-CSF has been approved for the amelioration of chemotherapy induced neutropenia as well as for severe chronic neutropenia following marrow transplant.

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Citations

1. Notch-dependent repression of miR-155 in the bone marrow niche regulates hematopoiesis in an NF-kappaB-dependent manner.
   Authors: Wang L, Zhang H, Rodriguez S, Cao L, Parish J, Mumaw C, Zollman A, Kamoka M, Mu J, Chen D, Srour E, Chitteti B, HogenEsch H, Tu X, Bellido T, Boswell H, Manshouri T, Verstovsek S, Yoder M, Kapur R, Cardoso A, Carlesso N
   Cell Stem Cell, 2014;15(1):51-65.
   Species: Mouse
   Sample Type: In Vivo
   Application: Neut

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