抗IL-1 beta/IL-1F2抗体(Anti-IL-1β, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

目次に戻る

Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse IL-1 beta /IL-1F2Val118-Ser269Accession # P10749
Source	Polyclonal Goat IgG
Purification	Protein A or G purified
Specificity	Detects mouse IL-1 beta /IL-1F2 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant cotton rat IL-1 beta and recombinant rhesus monkey IL-1 beta is observed, approximately 15% cross-reactivity with recombinant rat IL-1 beta and recombinant rabbit IL-1 beta is observed, and less than 10% cross-reactivity with recombinant canine IL-1 beta, recombinant equine IL-1 beta, recombinant guinea pig IL-1 beta, recombinant porcine IL-1 beta, recombinant feline IL-1 beta, and recombinant human IL-1 beta is observed.

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Simple Western	50 µg/mL	See below
Neutralization	Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line [Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272]. The Neutralization Dose (ND50) is typically 2-12 µg/mL in the presence of 10 pg/mL Recombinant Mouse IL‑1 beta /IL‑1F2 and 1.25 µg/mL concanavalin A.

Neutralization
	Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutral­ization by Mouse IL‑1 beta /IL‑1F2 Antibody. Recombinant Mouse IL‑1 beta /IL‑1F2 (Catalog # 401-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑1 beta /IL‑1F2 (10 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑1 beta /IL‑1F2 Polyclonal Antibody (Catalog # AB-401-NA). The ND50 is typically 2-12 µg/mL in the presence of concanavalin A (1.25 µg/mL).
Western Blot
	Detection of Human and MouseIL‑1 beta /IL‑1F2 by Western Blot. Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hours and 10 µg/mL LPS for 4 hours and RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 24 hours. PVDF membrane was probed with  µg/mL of Goat Anti-MouseIL‑1 beta /IL‑1F2 Polyclonal Antibody (Catalog # AB-401-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL‑1 beta /IL‑1F2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western
	Detection of Mouse IL‑1 beta /IL‑1F2 by Simple WesternTM. Simple Western lane view shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 24 hours, loaded at 0.5 mg/mL. A specific band was detected for IL‑1 beta /IL‑1F2 at approximately 40 kDa (as indicated) using 50 µg/mL of Goat Anti-Mouse IL‑1 beta /IL‑1F2 Polyclonal Antibody (Catalog # AB-401-NA). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

目次に戻る

Related Product & Information

Long Name	Interleukin 1 beta
Entrez Gene IDs	3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Background	IL-1 beta/IL-1F2
background_content	Background: IL-1 beta/IL-1F2 IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 17% amino acid (aa) identity in mouse. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The mouse IL-1 beta cDNA encodes a 269 aa precursor. A 117 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature mouse IL-1 beta shares 90% aa sequence identity with cotton rat and rat and 65-78% identity with canine, equine, feline, human, porcine, and rhesus macaque IL-1 beta.

目次に戻る

Citations

1. A small-molecule inhibitor of the NLRP3 inflammasome for the treatment of inflammatory diseases.
   Authors: Coll R, Robertson A, Chae J, Higgins S, Munoz-Planillo R, Inserra M, Vetter I, Dungan L, Monks B, Stutz A, Croker D, Butler M, Haneklaus M, Sutton C, Nunez G, Latz E, Kastner D, Mills K, Masters S, Schroder K, Cooper M, O'Neill L
   Nat Med, 2015;21(3):248-55.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


2. P2RX7 sensitizes Mac-1/ICAM-1-dependent leukocyte-endothelial adhesion and promotes neurovascular injury during septic encephalopathy.
   Authors: Wang H, Hong L, Huang J, Jiang Q, Tao R, Tan C, Lu N, Wang C, Ahmed M, Lu Y, Liu Z, Shi W, Lai E, Wilcox C, Han F
   Cell Res, 2015;25(6):674-90.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


3. Distinct mechanisms of induction of hepatic growth hormone resistance by endogenous IL-6, TNF-alpha, and IL-1beta.
   Authors: Zhao Y, Xiao X, Frank S, Lin H, Xia Y
   Am J Physiol Endocrinol Metab, 2014;307(2):E186-98.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


4. Interleukin-1beta and fibroblast growth factor receptor 1 cooperate to induce cyclooxygenase-2 during early mammary tumourigenesis.
   Authors: Reed JR, Leon RP, Hall MK, Schwertfeger KL
   Breast Cancer Res., 2009;11(2):R21.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


5. Comparison of the roles of IL-1, IL-6, and TNFalpha in cell culture and murine models of aseptic loosening.
   Authors: Taki N, Tatro JM, Lowe R, Goldberg VM, Greenfield EM
   Bone, 2007;40(5):1276-83.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


6. The Myc-dependent angiogenic switch in tumors is mediated by interleukin 1beta.
   Authors: Shchors K, Shchors E, Rostker F, Lawlor ER, Brown-Swigart L, Evan GI
   Genes Dev., 2006;20(18):2527-38.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo

目次に戻る