抗MIP-3β抗体(Anti-MIP-3β, Human, Goat-Poly antibody)
掲載日情報：2021/01/28 現在Webページ番号：26945
MIP-3βに対する抗体(Anti-MIP-3β, Human, Goat-Poly )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 ：2025年04月26日 20時35分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-MIP-3β, Human, Goat-Poly |
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Anti-Human CCL19/MIP-3 beta Affinity Purified Polyclonal Ab |
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[在庫・価格 ：2025年04月26日 20時35分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-MIP-3β, Human, Goat-Poly
文献数： 9
- 商品コード：AF361
- メーカー：RSD
- 包装：100μg
- 価格：¥107,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Human CCL19/MIP-3β サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAF361，スタンダードとして#361-MI-025を用いる。 別名：beta chemokine exodus-3 Genbank No： 6363 Protein Accession No： Q99731 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | Human | 免疫動物 | Goat | ||||
| 交差性 | Human | 適用 | ELISA,Neutralising,Simple Western,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | |||||||
| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Human CCL19/MIP-3 beta Affinity Purified Polyclonal Ab
文献数： 0
- 商品コード：AF361-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | ※受注発注品。形状：溶液または凍結乾燥 別名：beta chemokine exodus-3 Genbank No： 6363 Protein Accession No： Q99731 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Goat | |||||
| 交差性 | Human | 適用 | ELISA,Neutralising,Simple Western,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | M1/M2 Macrophage Activation Marker 使いっきり抗体 |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Human |
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| Label | Unconjugated |
| Immunogen | E. coli-derived recombinant human CCL19/MIP-3 beta (R&D Systems, Catalog # 361-MI)Gly22-Ser98Accession # Q99731 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects human CCL19/MIP-3 beta in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant mouse (rm) MIP‑3 beta, recombinant human (rh) GRO alpha, rhHCC-4, rhEotaxin, rmMIP-3 alpha, recombinant rat MIP-3 alpha, and rhMIP-3 alpha is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 1 µg/mL | See below |
| Simple Western | 50 µg/mL | See below |
| Human CCL19/MIP-3 beta Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 0.2-0.8 µg/mL | Human CCL19/MIP‑3 beta Antibody (Catalog #AF361 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Human CCL19/MIP‑3 beta Biotinylated Antibody (Catalog #BAF361 ) |
| ELISA Standard | Recombinant Human CCL19/MIP-3 beta Protein (Catalog #361-MI ) | |
| Neutralization | Measured by its ability to neutralize CCL19/MIP‑3 beta -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR7. The Neutralization Dose (ND50) is typically 2-10 µg/mL in the presence of 50 ng/mL Recombinant Human CCL19/MIP‑3 beta. | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Western Blot |
![Western Blot CCL19/MIP-3 beta Antibody [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/CCL19_AF361_Western_Blot_19575.jpg) click image to view larger | Detection of Human CCL19/MIP‑3 beta by Western Blot. Western blot shows conditioned media from monocyte-derived macrophages untreated (-) or treated (+) with 1 μg/mL LPS and 40 ng/mL Recombinant Human IFN‑ gamma (Catalog # 285-IF) for 24 hours. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CCL19/MIP‑3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF361) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CCL19/MIP‑3 beta at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
| Neutralization |
![Neutralization CCL19/MIP-3 beta Antibody [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/CCL19_AF361_Block_Neutralize_8856.jpg) click image to view larger | Chemotaxis Induced by CCL19/MIP‑3 beta and Neutralization by Human CCL19/MIP‑3 beta Antibody.Recombinant Human CCL19/MIP‑3 beta (Catalog # 361-MI) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL19/MIP‑3 beta (50 ng/mL) is neutralized (green line) by increasing concentrations of Human CCL19/MIP‑3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF361). The ND50 is typically 2-10 µg/mL. |
| Simple Western |
![Simple Western CCL19/MIP-3 beta Antibody [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/CCL19_AF361_Simple_Western_19576.jpg) click image to view larger | Detection of Human CCL19/MIP‑3 beta by Simple WesternTM. Simple Western lane view shows conditioned media from monocyte-derived macrophages untreated (-) or treated (+) with 1 μg/mL LPS and 40 ng/mL Recombinant Human IFN‑ gamma (Catalog # 285-IF) for 24 hours. A specific band was detected for CCL19/MIP‑3 beta at approximately 8 kDa (as indicated) using 50 µg/mL of Goat Anti-Human CCL19/MIP‑3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF361) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: CCL19/MIP-3 beta | MIP-3 beta, also known as ELC (EBI1-Ligand Chemokine), is one of many novel beta chemokines identified through bioinformatics. MIP-3 beta cDNA encodes a 98 amino acid (aa) residue precursor protein with a predicted 21 aa residue signal peptide that is cleaved to form the 77 aa residue mature secreted protein. MIP-3 beta is distantly related to other beta chemokines (20-30% aa sequence identity) and the gene for MIP-3 beta has been mapped to chromosome 9p13 rather than chromosome 17 where the genes for many human beta chemokines are clustered. MIP-3 beta has been shown to be constitutively expressed in various lymphoid tissues (including thymus, lymph nodes, appendix and spleen). The expression of MIP-3 beta is down-regulated by the anti-inflammatory cytokine IL-10. MIP-3 beta has been shown to be a unique functional ligand for CCR7 (previously referred to as the Epstein-Barr virus-induced gene 1 (EBI1) orphan receptor), a chemokine receptor that is expressed in various lymphoid tissues and activated B and T lymphocytes. EBI1 is strongly up-regulated in B cells infected with Epstein-Barr virus and T cells infected with herpesvirus 6 or 7. |
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| Entrez Gene IDs: | 6363 (Human); 24047 (Mouse); 362506 (Rat) | |
| Alternate Names: | beta chemokine exodus-3; Beta-chemokine exodus-3; CC chemokine ligand 19; C-C motif chemokine 19; CCL19; chemokine (C-C motif) ligand 19; CKb11; EBI1-ligand chemokine; ELC; ELCMIP-3-beta; Epstein-Barr virus-induced molecule 1 ligand chemokine; exodus-3; Macrophage inflammatory protein 3 beta; macrophage inflammatory protein 3-beta; MGC34433; MIP3 beta; MIP-3 beta; MIP-3b; MIP3BCK beta-11; SCYA19EBI1 ligand chemokine; small inducible cytokine subfamily A (Cys-Cys), member 19; Small-inducible cytokine A19 | |
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Related Research Areas
| B Cell Chemotaxis, Migration, and Adhesion | ||
| CC Chemokines and Receptors | ||
| Chemokines and Receptors | ||
| Chemokines and Receptors in Angiogenesis | ||
| Chemokines and Receptors in Tfh Cell Differentiation and Function | ||
| Macrophage Activation Markers | ||
| Molecules Secreted by VSMC | ||
| Regulatory T Cells (Tregs) | ||
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| Western Blot | |
|---|---|
 | Detection of Human CCL19/MIP‑3 beta by Western Blot. Western blot shows conditioned media from monocyte-derived macrophages untreated (-) or treated (+) with 1 μg/mL LPS and 40 ng/mL Recombinant Human IFN‑ gamma (Catalog # 285-IF) for 24 hours. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CCL19/MIP‑3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF361) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CCL19/MIP‑3 beta at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
| Neutralization | |
 | Chemotaxis Induced by CCL19/MIP‑3 beta and Neutralization by Human CCL19/MIP‑3 beta Antibody. Recombinant Human CCL19/MIP‑3 beta (Catalog # 361-MI) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL19/MIP‑3 beta (50 ng/mL) is neutralized (green line) by increasing concentrations of Human CCL19/MIP‑3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF361). The ND50 is typically 2-10 µg/mL. |
| Simple Western | |
 | Detection of Human CCL19/MIP‑3 beta by Simple WesternTM. Simple Western lane view shows conditioned media from monocyte-derived macrophages untreated (-) or treated (+) with 1 μg/mL LPS and 40 ng/mL Recombinant Human IFN‑ gamma (Catalog # 285-IF) for 24 hours. A specific band was detected for CCL19/MIP‑3 beta at approximately 8 kDa (as indicated) using 50 µg/mL of Goat Anti-Human CCL19/MIP‑3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF361) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
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Related Product & Information
| Entrez Gene IDs | 6363 (Human); 24047 (Mouse); 362506 (Rat) |
|---|---|
| Background | CCL19/MIP-3 beta |
| background_content | Background: CCL19/MIP-3 beta MIP-3 beta, also known as ELC (EBI1-Ligand Chemokine), is one of many novel beta chemokines identified through bioinformatics. MIP-3 beta cDNA encodes a 98 amino acid (aa) residue precursor protein with a predicted 21 aa residue signal peptide that is cleaved to form the 77 aa residue mature secreted protein. MIP-3 beta is distantly related to other beta chemokines (20-30% aa sequence identity) and the gene for MIP-3 beta has been mapped to chromosome 9p13 rather than chromosome 17 where the genes for many human beta chemokines are clustered. MIP-3 beta has been shown to be constitutively expressed in various lymphoid tissues (including thymus, lymph nodes, appendix and spleen). The expression of MIP-3 beta is down-regulated by the anti-inflammatory cytokine IL-10. MIP-3 beta has been shown to be a unique functional ligand for CCR7 (previously referred to as the Epstein-Barr virus-induced gene 1 (EBI1) orphan receptor), a chemokine receptor that is expressed in various lymphoid tissues and activated B and T lymphocytes. EBI1 is strongly up-regulated in B cells infected with Epstein-Barr virus and T cells infected with herpesvirus 6 or 7. |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
- Age-Related Changes to Human Tear Composition
Authors: A Micera, A Di Zazzo, G Esposito, R Longo, W Foulsham, R Sacco, R Sgrulletta, S Bonini
Invest. Ophthalmol. Vis. Sci., 2018;59(5):2024-2031. - Association of MIF, but not type I interferon-induced chemokines, with increased disease activity in Asian patients with systemic lupus erythematosus
Sci Rep, 2016;6(0):29909.
Species: Human
Sample Type: Serum - Expression of podoplanin and other lymphatic markers in the human anterior eye segment.
Authors: Birke K, Lutjen-Drecoll E, Kerjaschki D, Birke MT
Invest. Ophthalmol. Vis. Sci., 2010;51(1):344-54.
Species: Human
Sample Type: Whole Cells
Application: ICC - CXCL12 is essential for migration of activated Langerhans cells from epidermis to dermis.
Authors: Ouwehand K, Santegoets SJ, Bruynzeel DP, Scheper RJ, de Gruijl TD, Gibbs S
Eur. J. Immunol., 2008;38(11):3050-9.
Species: Human
Sample Type: Ex Vivo
Application: Blocking - Autologous chemotaxis as a mechanism of tumor cell homing to lymphatics via interstitial flow and autocrine CCR7 signaling.
Authors: Shields JD, Fleury ME, Yong C, Tomei AA, Randolph GJ, Swartz MA
Cancer Cell, 2007;11(6):526-38.
Species: Human
Sample Type: Whole Tissue
Application: IHC
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