抗MIP-3α抗体(Anti-MIP-3α, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human CCL20/MIP-3 alpha Ala27-Met96 (Asn95Asp)Accession # P78556
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human CCL20/MIP-3 alpha in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse Teck is observed.

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Applications and Data

	Recommended Concentration	Sample
Immunohistochemistry	5-15 µg/mL	See below
Immunocytochemistry	5-15 µg/mL	See below
Neutralization	Measured by its ability to neutralize CCL20/MIP‑3 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR6. The Neutralization Dose (ND50) is typically 0.2-1.0 µg/mL in the presence of 10 ng/mL Recombinant Human CCL20/MIP‑3 alpha.

Immunocytochemistry
	CCL20/MIP‑3 alpha in Human PBMCs. CCL20/MIP‑3 alpha was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stumulated with LPS using Human CCL20/MIP‑3 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF360) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Immunohistochemistry
	CCL20/MIP‑3 alpha in Human Skin. CCL20/MIP‑3 alpha was detected in immersion fixed paraffin-embedded sections of human skin using Goat Anti-Human CCL20/MIP‑3 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF360) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Neutralization
	Chemotaxis Induced by CCL20/MIP‑3 alpha and Neutralization by Human CCL20/MIP‑3 alpha Antibody. Recombinant Human CCL20/MIP‑3 alpha (Catalog # 360-MP) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL20/MIP‑3 alpha (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL20/MIP‑3 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF360). The ND50 is typically 0.2-1.0 µg/mL.

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Related Product & Information

Entrez Gene IDs	6364 (Human); 20297 (Mouse); 29538 (Rat)
Background	CCL20/MIP-3 alpha
background_content	Background: CCL20/MIP-3 alpha CCL20, also known as LARC (Liver and Activation-regulated Chemokine) and as Exodus, is one of many novel beta chemokines identified through bioinformatics. CCL20 cDNA encodes a 96 amino acid (aa) residue precursor protein with a 26 aa residue signal peptide that is predicted to be cleaved to form the 70 aa residue mature secreted protein. CCL20 is distantly related to other beta chemokines (20 - 28% aa sequence identity) and the gene for CCL20 has been mapped to chromosome 2 rather than 17. CCL20 has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. The expression of CCL20 is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL-10. Synthetic or recombinant CCL20 has been shown to be chemotactic for lymphocytes and to inhibit proliferation of myeloid progenitors in colony formation assays. CCL20 has now been shown to be a unique functional ligand for CCR-6 (previously referred to as GPR-CY4, CKR-L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34+ cord blood precursors.

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Citations

1. Fusobacterium nucleatum subspecies animalis influences pro-inflammatory cytokine expression and monocyte activation in human colorectal tumors
   Authors: X Ye, R Wang, R Bhattachar, D Boulbes, F Fan, L Xia, H Adoni, NJ Ajami, MC Wong, DP Smith, JF Petrosino, S Venable, W Qiao, V Baladanday, DM Maru, LM Ellis
   Cancer Prev Res (Phila), 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


2. Role of CCL20/CCR6 and the ERK signaling pathway in lung adenocarcinoma
   Authors: XP Zhang, ZJ Hu, AH Meng, GC Duan, QT Zhao, J Yang
   Oncol Lett, 2017;14(6):8183-8189.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC-P


3. Transforming Growth Factor-beta and Interleukin-1beta Signaling Pathways Converge on the Chemokine CCL20 Promoter.
   Authors: Brand O, Somanath S, Moermans C, Yanagisawa H, Hashimoto M, Cambier S, Markovics J, Bondesson A, Hill A, Jablons D, Wolters P, Lou J, Marks J, Baron J, Nishimura S
   J Biol Chem, 2015;290(23):14717-28.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC - Paraffin embedded


4. Microarray analysis of gene expression by microdissected epidermis and dermis in mycosis fungoides and adult T-cell leukemia/lymphoma.
   Authors: Hashikawa K, Yasumoto S, Nakashima K, Arakawa F, Kiyasu J, Kimura Y, Saruta H, Nakama T, Yasuda K, Tashiro K, Kuhara S, Hashimoto T, Ohshima K
   Int J Oncol, 2014;45(3):1200-8.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC - paraffin


5. CCR6, the sole receptor for the chemokine CCL20, promotes spontaneous intestinal tumorigenesis.
   Authors: Nandi, Bisweswa, Pai, Christin, Huang, Qin, Prabhala, Rao H, Munshi, Nikhil C, Gold, Jason S
   PLoS ONE, 2014;9(5):e97566.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


6. IL-22 negatively regulates Helicobacter pylori-induced CCL20 expression in gastric epithelial cells.
   Authors: Chen J, Wu M, Kuo S, Liao F
   PLoS ONE, 2014;9(5):e97350.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


7. EBV infection is common in gingival epithelial cells of the periodontium and worsens during chronic periodontitis.
   Authors: Vincent-Bugnas S, Vitale S, Mouline C, Khaali W, Charbit Y, Mahler P, Precheur I, Hofman P, Maryanski J, Doglio A
   PLoS ONE, 2013;8(12):e80336.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


8. Lung myeloid dendritic cells coordinately induce TH1 and TH17 responses in human emphysema.
   Authors: Shan M, Cheng HF, Song LZ
   Sci Transl Med, 2009;1(4):4ra10.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


9. Macrophage inflammatory protein-3alpha is a novel serum marker for nasopharyngeal carcinoma detection and prediction of treatment outcomes.
   Authors: Chang KP, Hao SP, Chang JH, Wu CC, Tsang NM, Lee YS, Hsu CL, Ueng SH, Liu SC, Liu YL, Wei PC, Liang Y, Chang YS, Yu JS
   Clin. Cancer Res., 2008;14(21):6979-87.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


10. Substance P regulates macrophage inflammatory protein 3alpha/chemokine C-C ligand 20 (CCL20) with heme oxygenase-1 in human periodontal ligament cells.
    Authors: Lee SK, Pi SH, Kim SH, Min KS, Lee HJ, Chang HS, Kang KH, Kim HR, Shin HI, Lee SK, Kim EC
    Clin. Exp. Immunol., 2007;150(3):567-75.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development

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