抗Flk-1抗体(Anti-Flk-1, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human VEGF R2/KDR/Flk-1Ala20-Glu764Accession # AAC16450
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human VEGF R2/KDR/Flk‑1 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse VEGF R2 is observed and less than 10% cross-reactivity with recombinant human (rh) VEGF R1 and rhVEGF R3 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below
Neutralization	Measured by its ability to neutralize VEGF R2/KDR/Flk‑1-mediated inhibition of proliferation in HUVEC human umbilical vein endothelial cells. The Neutralization Dose (ND50) is typically 0.05-0.25 µg/mL in the presence of 30 ng/mL Recombinant Human VEGF R2/KDR/Flk‑1 Fc Chimera and 5 ng/mL Recombinant Human VEGF165.

Western Blot
	Detection of Human VEGF R2/KDR/Flk‑1 by Western Blot. Western blot shows lysate of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human VEGF R2/KDR/Flk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF357) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for VEGF R2/KDR/Flk‑1 at approximately 200-250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
	VEGF R2/KDR/Flk‑1 in Human Placenta. VEGF R2/KDR/Flk‑1 was detected in immersion fixed paraffin-embedded sections of human placenta using 15 µg/mL Goat Anti-Human VEGF R2/KDR/Flk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF357) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
	VEGF R2/KDR/Flk‑1 in Human Kidney. VEGF R2/KDR/Flk‑1 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human VEGF R2/KDR/Flk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF357) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Neutralization
	VEGF R2/KDR/Flk‑1 Inhibi­tion of VEGF-dependent Cell Proliferation and Neutral­ization by Human VEGF R2/KDR/Flk‑1 Anti­body. Recombi­nant Human VEGF R2/KDR/Flk‑1 Fc Chi­mera (Catalog # 357-KD) inhibits Recombinant Human VEGF165 (Catalog # 293-VE) induced proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Inhibition of Recombinant Human VEGF165 (5 ng/mL) activity elicited by Recombinant Human VEGF R2/KDR/Flk‑1 Fc Chi­mera (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human VEGF R2/KDR/Flk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF357). The ND50 is typically0.05‑0.25 µg/mL.

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Related Product & Information

Long Name	Vascular Endothelial Growth Factor Receptor 2
Entrez Gene IDs	3791 (Human); 16542 (Mouse)
Background	VEGF R2/KDR/Flk-1
background_content	Background: VEGF R2/KDR/Flk-1 VEGF R2 (KDR/Flk-1), VEGF R1 (Flt-1) and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGF R1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. VEGF R2 cDNA encodes a 1356 amino acid (aa) residue precursor protein with a 19 aa residue signal peptide. Mature VEGF R2 is composed of a 745 aa residue extracellular domain, a 25 aa residue transmembrane domain and a 567 aa residue cytoplasmic domain. In contrast to VEGF R1 which binds both PlGF and VEGF with high affinity, VEGF R2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGF R2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist.

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Citations

1. VEGFR3 Modulates Vascular Permeability by Controlling VEGF/VEGFR2 Signaling
   Authors: K Heinolaine, S Karaman, G D'Amico, T Tammela, R Sormunen, L Eklund, K Alitalo, G Zarkada
   Circ. Res, 2017;0(0):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


2. Endoglin prevents vascular malformation by regulating flow-induced cell migration and specification through VEGFR2 signalling
   Authors: Y Jin, L Muhl, M Burmakin, Y Wang, AC Duchez, C Betsholtz, HM Arthur, L Jakobsson
   Nat. Cell Biol., 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


3. Affimer proteins are versatile and renewable affinity reagents
   Authors: C Tiede, R Bedford, SJ Heseltine, G Smith, I Wijetunga, R Ross, D AlQallaf, AP Roberts, A Balls, A Curd, RE Hughes, H Martin, SR Needham, LC Zanetti-Do, Y Sadigh, TP Peacock, AA Tang, N Gibson, H Kyle, GW Platt, N Ingram, T Taylor, LP Coletta, I Manfield, M Knowles, S Bell, F Esteves, A Maqbool, RK Prasad, M Drinkhill, RS Bon, V Patel, SA Goodchild, M Martin-Fer, RJ Owens, JE Nettleship, ME Webb, M Harrison, JD Lippiat, S Ponnambala, M Peckham, A Smith, PK Ferrigno, M Johnson, MJ McPherson, DC Tomlinson
   Elife, 2017;6(0):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


4. Lymphatic endothelial progenitors originate from plastic myeloid cells activated by toll-like receptor-4
   Authors: LD Volk-Drape, KL Hall, AC Wilber, S Ran
   PLoS ONE, 2017;12(6):e0179257.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


5. VEGF induces signalling and angiogenesis by directing VEGFR2 internalisation via macropinocytosis
   J Cell Sci, 2016;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


6. Control of angiogenesis by galectins involves the release of platelet-derived proangiogenic factors.
   Authors: Etulain J, Negrotto S, Tribulatti M, Croci D, Carabelli J, Campetella O, Rabinovich G, Schattner M
   PLoS ONE, 2014;9(4):e96402.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


7. Differential sensitivity of prostate tumor derived endothelial cells to sorafenib and sunitinib.
   Authors: Fiorio Pla, Alessand, Brossa, Alessia, Bernardini, Michela, Genova, Tullio, Grolez, Guillaum, Villers, Arnaud, Leroy, Xavier, Prevarskaya, Natalia, Gkika, Dimitra, Bussolati, Benedett
   BMC Cancer, 2014;14(0):939.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


8. Endothelial cell adhesion to soluble vascular endothelial growth factor receptor-1 triggers a cell dynamic and angiogenic phenotype.
   Authors: Orecchia A, Mettouchi A, Uva P, Simon G, Arcelli D, Avitabile S, Ragone G, Meneguzzi G, Pfenninger K, Zambruno G, Failla C
   FASEB J, 2014;28(2):692-704.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


9. VEGF-A isoforms differentially regulate ATF-2-dependent VCAM-1 gene expression and endothelial-leukocyte interactions.
   Authors: Fearnley G, Odell A, Latham A, Mughal N, Bruns A, Burgoyne N, Homer-Vanniasinkam S, Zachary I, Hollstein M, Wheatcroft S, Ponnambalam S
   Mol Biol Cell, 2014;25(16):2509-21.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


10. Sustained delivery of VEGF maintains innervation and promotes reperfusion in ischemic skeletal muscles via NGF/GDNF signaling.
    Authors: Shvartsman, Dmitry, Storrie-White, Hannah, Lee, Kangwon, Kearney, Cathal, Brudno, Yevgeny, Ho, Nhi, Cezar, Christin, McCann, Corey, Anderson, Erin, Koullias, John, Tapia, Juan Car, Vandenburgh, Herman, Lichtman, Jeff W, Mooney, David J
    Mol Ther, 2014;22(7):1243-53.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay

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