抗Flt-4抗体(Anti-Flt-4, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human VEGF R3Tyr25-Ile776Accession # P35916
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human VEGF R3 in direct ELISAs and Western blots. In Western blots, approximately 15% cross-reactivity with recombinant mouse VEGF R3 is observed and less than 2% cross-reactivity with recombinant human VEGF R1 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human VEGF R3/Flt‑4 Fc Chimera (Catalog # 349-F4)
Immunohistochemistry	5-15 µg/mL	See below

Immunohistochemistry

VEGF R3/Flt‑4 in Human Cervical Squamous Metaplasia. VEGF R3/Flt‑4 was detected in immersion fixed paraffin-embedded sections of human cervical squamous metaplasia using Human VEGF R3/Flt‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF349) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

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Related Product & Information

Long Name	Vascular Endothelial Growth Factor Receptor 3
Entrez Gene IDs	2324 (Human); 14257 (Mouse)
Background	VEGF R3/Flt-4
background_content	Background: VEGF R3/Flt-4 VEGF R2 (KDR/Flk-1), VEGF R1 (Flt-1) and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGF R1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. VEGF R3 cDNA encodes a 1298 amino acid (aa) residue precursor protein with a 24 aa residue signal peptide. Mature VEGF R3 is composed of a 751 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 482 aa residue cytoplasmic domain. Both VEGF-C and VEGF-D have been shown to bind and activate VEGF R3 (Flt-4). VEGF R3 is widely expressed in the early embryo but becomes restricted to lymphatic endothelia at later stages of development. It is likely that VEGF R3 may be important for lymph angiogenesis.

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Citations

1. Lymphatic endothelial progenitors originate from plastic myeloid cells activated by toll-like receptor-4
   Authors: LD Volk-Drape, KL Hall, AC Wilber, S Ran
   PLoS ONE, 2017;12(6):e0179257.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. VEGFR3 Modulates Vascular Permeability by Controlling VEGF/VEGFR2 Signaling
   Authors: K Heinolaine, S Karaman, G D'Amico, T Tammela, R Sormunen, L Eklund, K Alitalo, G Zarkada
   Circ. Res, 2017;0(0):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. TGF-?1 Is Present at High Levels in Wound Fluid from Breast Cancer Patients Immediately Post-Surgery, and Is Not Increased by Intraoperative Radiation Therapy (IORT)
   PLoS ONE, 2016;11(9):e0162221.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


4. Effects of neuregulin GGF2 (cimaglermin alfa) dose and treatment frequency on left ventricular function in rats following myocardial infarction
   Authors: Tom J Parry
   Eur. J. Pharmacol, 2016;796(0):76-89.
   Species: Human
   Sample Type: Recombinant Protein
   Application: WB


5. Specific niches for lung-resident memory CD8+ T cells at the site of tissue regeneration enable CD69-independent maintenance
   Authors: Masaaki Miyazawa
   J. Exp. Med., 2016;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC - Frozen


6. Primary Tumor-Secreted Lymphangiogenic Factors Induce Pre-Metastatic Lymphvascular Niche Formation at Sentinel Lymph Nodes in Oral Squamous Cell Carcinoma.
   Authors: Wakisaka N, Hasegawa Y, Yoshimoto S, Miura K, Shiotani A, Yokoyama J, Sugasawa M, Moriyama-Kita M, Endo K, Yoshizaki T
   PLoS ONE, 2015;10(12):e0144056.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


7. Expression of vascular endothelial growth factor-C and vascular endothelial growth factor receptor-3 in esophageal squamous cell carcinoma.
   Authors: Omoto, Itaru, Matsumoto, Masataka, Okumura, Hiroshi, Uchikado, Yasuto, Setoyama, Tetsuro, Kita, Yoshiaki, Owaki, Tetsuhir, Kijima, Yuko, Shinchi, Hiroyuki, Ishigami, Sumiya, Ueno, Shinichi, Natsugoe, Shoji
   Oncol Lett, 2014;7(4):1027-1032.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


8. VEGF-D promotes tumor growth and lymphatic spread in a mouse model of hepatocellular carcinoma.
   Authors: Thelen A, Scholz A, Benckert C, Von Marschall Z, Schroder M, Wiedenmann B, Neuhaus P, Rosewicz S, Jonas S
   Int. J. Cancer, 2008;122(11):2471-81.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


9. Cyclooxygenase-2 induces EP1- and HER-2/Neu-dependent vascular endothelial growth factor-C up-regulation: a novel mechanism of lymphangiogenesis in lung adenocarcinoma.
   Authors: Su JL, Shih JY, Yen ML, Jeng YM, Chang CC, Hsieh CY, Wei LH, Yang PC, Kuo ML
   Cancer Res., 2004;64(2):554-64.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


10. All three receptors for vascular endothelial growth factor (VEGF) are expressed on B-chronic lymphocytic leukemia (CLL) cells.
    Authors: Bairey O, Boycov O, Kaganovsky E, Zimra Y, Shaklai M, Rabizadeh E
    Leuk. Res., 2004;28(3):243-8.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC

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