抗IL-17抗体(Anti-IL-17, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human IL-17Ile20-Ala155Accession # Q16552
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human IL-17 in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross-reactivity with recombinant canine IL-17 is observed, approximately 10% cross-reactivity with recombinant human (rh) IL-17F and with recombinant mouse IL‑17 is observed, and less than 1% cross-reactivity with rhIL-17B, rhIL-17C, rhIL-17D, and rhIL‑17E is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Immunohistochemistry	1-15 µg/mL	See below
Immunoprecipitation	3 µg/100 µg cell lysate	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	5-15 µg/mL	Immersion fixed human peripheral blood mononuclear cells treated with PHA
Intracellular Staining by Flow Cytometry	2.5 µg/106 cells	See below
Neutralization	Measured by its ability to neutralize IL‑17-induced IL‑6 secretion in NIH/3T3 mouse embryonic fibroblasts. Yao, Z. et al. (1995) Immunity 3:811. The Neutralization Dose (ND50) is typically 0.02-0.12 µg/mL in the presence of 15 ng/mL Recombinant Human IL‑17.

Western Blot
	Detection of Human IL‑17 by Western Blot. Western blot shows lysates of human primary naïve CD4+ T cells, along with whole cell lysates (WCL) and conditioned-media supernatant (Supe) of human primary differentiated Th17 cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-HumanIL‑17 Antigen Affinity-purified Polyclonal Antibody(Catalog # AF‑317‑NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL‑17 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
	IL‑17/IL‑17A in Human Tonsil. IL‑17/IL‑17A was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human IL‑17/IL‑17A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Immunoprecipitation
	Immunoprecipitation of Human IL‑17. Human IL-17 was immunoprecipitated from 100 μg of human primary differentiated Th17 cell lysate following incubation with 3 µg Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA) or control antibody (Catalog # AB-108-C) overnight at 4 °C. IL-17-antibody complexes were absorbed using Protein G Sepharose. Immunoprecipitated IL-17 was detected by Western blot using 1 µg/mL Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA). View our recommended buffer recipes for immunoprecipitation.
Neutralization
	IL‑6 Secretion Induced by IL‑17 and Neutralization by Human IL‑17 Antibody. Recombinant Human IL‑17 (Catalog # 317-ILB) stimulates IL‑6 secretion in NIH/3T3 mouse embryonic fibroblasts in a dose-dependent manner (orange line), as measured by the mouse IL‑6 Quantikine ELISA Kit (Catalog # M6000B). IL‑6 secretion elicited by Recombinant Human IL‑17 (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA). The ND50 is typically0.02‑0.12 µg/mL.
Intracellular Staining by Flow Cytometry
	Detection of IL‑17 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells were unstimulated (light orange filled histogram) or treated with 50 ng/mL PMA and 250 ng/mL Ca2+ ionomycin for 16 hours, then stained with Goat Anti-Human IL‑17 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF‑317‑NA, dark orange filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

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Related Product & Information

Long Name	Interleukin 17
Entrez Gene IDs	3605 (Human); 16171 (Mouse); 301289 (Rat); 481837 (Canine)
Background	IL-17/IL-17A
background_content	Background: IL-17/IL-17A Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpesvirus Saimiri. cDNA clones encoding IL-17 have been isolated from activated rat, mouse and human T cells. Human IL-17 cDNA encodes a 155 amino acid (aa) residue precursor protein with a 19 amino acid residue signal peptide that is cleaved to yield the 136 aa residue mature IL-17 containing one potential N-linked glycosylation site. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. At the amino acid level, human IL-17 shows 72% and 63% sequence identity with herpesvirus and rat IL-17, respectively. An IL-17 specific mouse cell surface receptor (IL-17 R) has recently been cloned. While the expression of IL-17 mRNA is restricted to activated T cells, the expression of mIL-17 R mRNA has been detected in virtually all cells and tissues tested. IL-17 exhibits multiple biological activities on a variety of cells including the induction of IL-6 and IL-8 production in fibroblasts, the enhancement of surface expression of ICAM-1 in fibroblasts, activation of NF-kappa B and costimulation of T cell proliferation.

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Citations

1. Chronic hepatitis C liver microenvironment: role of the Th17/Treg interplay related to fibrogenesis
   Authors: DA Rios, P Valva, PC Casciato, S Frias, M Soledad Ca, MI Gaillard, L Bezrodnik, J Bandi, O Galdame, B Ameigeiras, D Krasniansk, C Brodersen, E Mullen, EN Matteo, MV Preciado
   Sci Rep, 2017;7(1):13283.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


2. Zoonotic intestinal helminths interact with the canine immune system by modulating T cell responses and preventing dendritic cell maturation
   Authors: J Junginger, K Raue, K Wolf, E Janecek, VM Stein, A Tipold, AR Günzel-Ape, C Strube, M Hewicker-T
   Sci Rep, 2017;7(1):10310.
   Species: Canine
   Sample Type: Whole Cells
   Application: Flow


3. Expression of IL-23/Th17-related cytokines in basal cell carcinoma and in the response to medical treatments
   Authors: C Pellegrini, A Orlandi, G Costanza, A Di Stefani, A Piccioni, A Di Cesare, A Chiricozzi, A Ferlosio, K Peris, MC Fargnoli
   PLoS ONE, 2017;12(8):e0183415.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC paraffin embedded


4. Peritoneal carcinomatosis of colorectal cancer is characterized by structural and functional reorganization of the tumor microenvironment inducing senescence and proliferation arrest in cancer cells
   Authors: CT Seebauer, S Brunner, G Glockzin, P Piso, P Ruemmele, HJ Schlitt, EK Geissler, S Fichtner-F, R Kesselring
   Oncoimmunology, 2016;5(12):e1242543.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC-P


5. IL-17A mediates inflammatory and tissue remodelling events in early human tendinopathy
   Authors: Neal L Millar
   Sci Rep, 2016;6(0):27149.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC - Not specified


6. Atopy and Inhaled Corticosteroid Use Associate with Fewer IL-17+ Cells in Asthmatic Airways
   PLoS ONE, 2016;11(8):e0161433.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC - Paraffin embedded


7. Functional expression of aryl hydrocarbon receptor on mast cells populating human endometriotic tissues
   Lab Invest, 2016;0(0):.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


8. RORgammat(+) hematopoietic cells are necessary for tumor cell proliferation during colitis-associated tumorigenesis in mice.
   Authors: Martin M, Kesselring R, Saidou B, Brunner S, Schiechl G, Mouris V, Wege A, Rummele P, Schlitt H, Geissler E, Fichtner-Feigl S
   Eur J Immunol, 2015;45(6):1667-79.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


9. Association of Lung Inflammatory Cells with Small Airways Function and Exhaled Breath Markers in Smokers - Is There a Specific Role for Mast Cells?
   Authors: Nussbaumer-Ochsner Y, Stolk J, Ferraz da Silva L, van Schadewijk A, de Jeu R, Prins F, Mauad T, Rabe K, Hiemstra P
   PLoS ONE, 2015;10(6):e0129426.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Not Specified


10. Correlations between immune response and vascularization qRT-PCR gene expression clusters in squamous cervical cancer.
    Authors: Punt S, Houwing-Duistermaat J, Schulkens I, Thijssen V, Osse E, de Kroon C, Griffioen A, Fleuren G, Gorter A, Jordanova E
    Mol Cancer, 2015;14(0):71.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC Paraffin-embedded

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