抗Tie-2抗体(Anti-Tie-2, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human Tie‑2Ala23-Lys745Accession # Q02763
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human Tie-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant mouse Tie-2 is observed and less than 1% cross-reactivity with recombinant human Tie-1 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Simple Western	10 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below
Blockade of Receptor-ligand Interaction	In a functional ELISA, 5-10 µg/mL of this antibody will block 50% of the binding of 40 ng/mL of biotinylated Recombinant Human Angiopoietin-2 (Catalog # BT623) to immobilized Recombinant Human Tie-2 Fc Chimera (Catalog # 313-TI) coated at 4 µg/mL (100 µL/well). At 50 μg/mL, this antibody will block >90% of the binding.

Western Blot
	Detection of Human Tie‑2 by Western Blot. Western blot shows lysate of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Tie‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF313) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Tie‑2 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
	Tie‑2 in Human Placenta. Tie‑2 was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human Tie‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF313) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Simple Western
	Detection of Human Tie‑2 by Simple WesternTM. Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, loaded at 0.2 mg/mL. A specific band was detected for Tie‑2 at approximately 161 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Tie‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF313) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Related Product & Information

Long Name	Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 2
Entrez Gene IDs	7010 (Human); 21687 (Mouse); 30747 (Zebrafish)
Background	Tie-2
background_content	Background: Tie-2 Tie-1/Tie (tyrosine kinase with Ig and EGF homology domains 1) and Tie-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis. Human Tie-2 cDNA encodes a 1124 amino acid (aa) residue precursor protein with an 18 residue putative signal peptide, a 727 residue extracellular domain and a 354 residue cytoplasmic domain. Two ligands, angiopoietin-1 (Ang-1) and angiopoietin-2 (Ang-2), which bind Tie-2 with high-affinity have been identified. Ang-2 has been reported to act as an antagonist for Ang-1. Mice engineered to overexpress Ang-2 or to lack Ang-1 or Tie-2 display similar angiogenesis defects.

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Citations

1. Structural basis of Tie2 activation and Tie2/Tie1 heterodimerization
   Authors: VM Leppänen, P Saharinen, K Alitalo
   Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


2. Endothelial destabilization by angiopoietin-2 via integrin beta1 activation.
   Authors: Hakanpaa L, Sipila T, Leppanen V, Gautam P, Nurmi H, Jacquemet G, Eklund L, Ivaska J, Alitalo K, Saharinen P
   Nat Commun, 2015;6(0):5962.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


3. slanDCs selectively accumulate in carcinoma-draining lymph nodes and marginate metastatic cells.
   Authors: Vermi W, Micheletti A, Lonardi S, Costantini C, Calzetti F, Nascimbeni R, Bugatti M, Codazzi M, Pinter P, Schakel K, Tamassia N, Cassatella M
   Nat Commun, 2014;5(0):3029.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


4. Chromatin structure of two genomic sites for targeted transgene integration in induced pluripotent stem cells and hematopoietic stem cells.
   Authors: van Rensburg, R, Beyer, I, Yao, X-Y, Wang, H, Denisenko, O, Li, Z-Y, Russell, D W, Miller, D G, Gregory, P, Holmes, M, Bomsztyk, K, Lieber, A
   Gene Ther, 2013;20(2):201-14.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


5. Exhaustion of nucleus pulposus progenitor cells with ageing and degeneration of the intervertebral disc.
   Authors: Sakai, Daisuke, Nakamura, Yoshihik, Nakai, Tomoko, Mishima, Taishi, Kato, Shunichi, Grad, Sibylle, Alini, Mauro, Risbud, Makarand, Chan, Danny, Cheah, Kathryn, Yamamura, Ken-ichi, Masuda, Koichi, Okano, Hideyuki, Ando, Kiyoshi, Mochida, Joji
   Nat Commun, 2012;3(0):1264.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


6. Opposing effects of the angiopoietins on the thrombin-induced permeability of human pulmonary microvascular endothelial cells.
   Authors: van der Heijden M, van Nieuw Amerongen GP, van Bezu J, Paul MA, Groeneveld AB, van Hinsbergh VW
   PLoS ONE, 2011;6(8):e23448.
   Species: Human
   Sample Type: Cell Lysates
   Application: IP


7. VEGF-DdeltaNdeltaC mediated angiogenesis in skeletal muscles of diabetic WHHL rabbits.
   Authors: Roy H, Bhardwaj S, Babu M, Lahteenvuo JE, Yla-Herttuala S
   Eur. J. Clin. Invest., 2010;40(5):422-32.
   Species: Rabbit
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


8. Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulation.
   Authors: Reikvam H, Hatfield KJ, Oyan AM, Kalland KH, Kittang AO, Bruserud O
   Eur. J. Haematol., 2010;84(3):239-51.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


9. Tumour vascularization via endothelial differentiation of glioblastoma stem-like cells.
   Authors: Ricci-Vitiani L, Pallini R, Biffoni M, Todaro M, Invernici G, Cenci T, Maira G, Parati EA, Stassi G, Larocca LM, De Maria R
   Nature, 2010;468(7325):824-8.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


10. The angiogenic factor angiopoietin-1 is a proneurogenic peptide on subventricular zone stem/progenitor cells.
    Authors: Rosa AI, Goncalves J, Cortes L, Bernardino L, Malva JO, Agasse F
    J. Neurosci., 2010;30(13):4573-84.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB

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