抗IGF-Ⅰ Receptor抗体(Anti-IGF-Ⅰ Receptor, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse
Label	Unconjugated
Immunogen	recombinant human IGF-I R extracellular domainAccession # P08069
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human and mouse IGF-I R in direct ELISAs and Western blots. In direct ELISAs and Western blots, 25-50% cross-reactivity with recombinant mouse IGF-I R is observed. In direct ELISAs, less than 1% cross-reactivity with recombinant human IGF‑II R is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human IGF-I R (Catalog # 391-GR) and recombinant Mouse IGF-I R.
Immunohistochemistry	5-15 µg/mL	See below
Immunocytochemistry	1-15 µg/mL	See below
Neutralization	Measured by its ability to neutralize IGF‑I-induced proliferation in the MCF‑7 human breast cancer cell line. Karey, K.P. et al. (1988) Cancer Research 48:4083. The Neutralization Dose (ND50) is typically 0.5-1.5 µg/mL in the presence of 6 ng/mL Recombinant Human IGF‑I.

Immunocytochemistry
	IGF‑I R in MCF‑7 and HDLM-2 Human Cell Lines. IGF‑I R was detected in immersion fixed MCF‑7 human breast cancer cell line (positive control, left panel) and HDLM‑2 human Hodgkin's lymphoma cell line (negative control, right panel) using Goat Anti-Human/Mouse IGF‑I R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-305-NA) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocols for Fluorescent ICC Staining of Cells on Coverslips and Fluorescent ICC Staining of Non-adherent Cells.
Immunohistochemistry
	IGF-I R in Mouse Embryo. IGF-I R was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Human IGF-I R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-305-NA) at 10 µg/mL overnight at4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Immunohistochemistry
	IGF‑I R in Human Placenta. IGF‑I R was detected in immersion fixed paraffin-embedded sections of human placenta (chorionic villi) using 15 µg/mL Goat Anti-Human IGF‑I R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑305‑NA) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Neutralization
	Cell Proliferation Induced by IGF‑I and Neutralization by Human IGF‑I R Antibody. Recombinant Human IGF‑I (Catalog # 291-G1) stimulates proliferation in the MCF‑7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IGF‑I (6 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IGF-I R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-305-NA). The ND50 is typically 0.5-1.5 µg/mL.

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Related Product & Information

Long Name	Insulin-like Growth Factor I Receptor
Entrez Gene IDs	3480 (Human)
Background	IGF-I R
background_content	Background: IGF-I R IGF-I receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I receptor is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. The IGF-I receptor is highly expressed in all cell types and tissues. Essentially all of the biological activities of IGF-I and II have been shown to be mediated via IGF-I R.

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Citations

1. Determination of Mammalian Target of Rapamycin Hyperactivation as Prognostic Factor in Well-Differentiated Neuroendocrine Tumors
   Authors: G Lamberti, C Ceccarelli, N Brighi, I Maggio, D Santini, C Mosconi, C Ricci, G Biasco, D Campana
   Gastroenterol Res Pract, 2017;2017(0):7872519.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC-P


2. Antibody-drug conjugates bearing pyrrolobenzodiazepine or tubulysin payloads are immunomodulatory and synergize with multiple immunotherapies
   Authors: J Rios-Doria, J Harper, R Rothstein, L Wetzel, J Chesebroug, AM Marrero, C Chen, P Strout, K Mulgrew, KA McGlinchey, R Fleming, B Bezabeh, J Meekin, D Stewart, M Kennedy, P Martin, A Buchanan, N Dimasi, EF Michelotti, RE Hollingswo
   Cancer Res, 2017;0(0):.
   Species: Rat
   Sample Type: Whole Cells
   Application: Functional Assay


3. Mycobacterium leprae-induced Insulin-like Growth Factor I attenuates antimicrobial mechanisms, promoting bacterial survival in macrophages
   Authors: L R Batista-Si
   Sci Rep, 2016;6(0):27632.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Luciferase Assay


4. Transplantation of Human Neural Progenitor Cells Expressing IGF-1 Enhances Retinal Ganglion Cell Survival.
   Authors: Ma J, Guo C, Guo C, Sun Y, Liao T, Beattie U, Lopez F, Chen D, Lashkari K
   PLoS ONE, 2015;10(4):e0125695.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


5. LMP1 promotes expression of insulin-like growth factor 1 (IGF1) to selectively activate IGF1 receptor and drive cell proliferation.
   Authors: Tworkoski K, Raab-Traub N
   J Virol, 2015;89(5):2590-602.
   Species: Human
   Sample Type: Cell Lysates
   Application: Neut


6. IGF-IR mediated mammary tumorigenesis is enhanced during pubertal development.
   Authors: Jones R, Watson K, Campbell C, Moorehead R
   PLoS ONE, 2014;9(9):e108781.
   


7. FGFR2 is amplified in the NCI-H716 colorectal cancer cell line and is required for growth and survival.
   Authors: Mathur, Anjili, Ware, Christop, Davis, Lenora, Gazdar, Adi, Pan, Bo-Sheng, Lutterbach, Bart
   PLoS ONE, 2014;9(6):e98515.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


8. Hyperactivation of the insulin-like growth factor receptor I signaling pathway is an essential event for cisplatin resistance of ovarian cancer cells.
   Authors: Eckstein N, Servan K, Hildebrandt B, Politz A, von Jonquieres G, Wolf-Kummeth S, Napierski I, Hamacher A, Kassack MU, Budczies J, Beier M, Dietel M, Royer-Pokora B, Denkert C, Royer HD
   Cancer Res., 2009;69(7):2996-3003.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


9. Differential expression of receptor tyrosine kinases (RTKs) and IGF-I pathway activation in human uterine leiomyomas.
   Authors: Yu L, Saile K, Swartz CD, He H, Zheng X, Kissling GE, Di X, Lucas S, Robboy SJ, Dixon D
   Mol. Med., 2008;14(5):264-75.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut

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