抗IGF-Ⅰ抗体(Anti-IGF-Ⅰ, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human IGF-I
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human IGF-I in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant mouse IGF-I and recombinant rat IGF-1 is observed, and less than 1% cross-reactivity with recombinant human (rh) IGF-2 and rhIGF-L1 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human IGF-I (Catalog # 291-G1)
Immunohistochemistry	5-15 µg/mL	See below
Immunocytochemistry	5-15 µg/mL	See below
Neutralization	Measured by its ability to neutralize IGF‑I-induced proliferation in the MCF‑7 human breast cancer cell line. Karey, K.P. et al. (1988) Cancer Research 48:4083. The Neutralization Dose (ND50) is typically 3-12 µg/mL in the presence of 6 ng/mL Recombinant Human IGF‑I.

Neutralization
	Cell Proliferation Induced by IGF‑I and Neutralization by Human IGF‑I Antibody. Recombinant Human IGF‑I (Catalog # 291-G1) stimulates proliferation in the MCF‑7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IGF‑I (6 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IGF-I Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA). The ND50 is typically 3‑12 µg/mL.
Immunocytochemistry
	IGF-I in MDA‑MB‑231 Human Cell Line. IGF-I was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Goat Anti-Human IGF-I Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
	IGF‑I in Human Placenta. IGF‑I was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human IGF‑I Antigen Affinity-purified Polyclonal Antibody (Catalog #AF-291-NA) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblasts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

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Related Product & Information

Long Name	Insulin-like Growth Factor I
Entrez Gene IDs	3479 (Human); 16000 (Mouse); 24482 (Rat)
Background	IGF-I
background_content	Background: IGF-I IGF-I belongs to the family of insulin-like growth factors and circulates in complex with IGF binding proteins. It is a potent mitogenic growth factor that binds the heteromeric type I and type II IGF receptors. Essentially, all of the biological activities of IGF-I are mediated by IGF-I R.

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Citations

1. Aberrant expression of NKL homeobox gene HLX in Hodgkin lymphoma
   Authors: S Nagel, C Pommerenke, C Meyer, M Kaufmann, RAF MacLeod, HG Drexler
   Oncotarget, 2018;9(18):14338-14353.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


2. Protein C receptor stimulates multiple signaling pathways in breast cancer cells
   Authors: D Wang, C Liu, J Wang, Y Jia, X Hu, H Jiang, ZM Shao, YA Zeng
   J. Biol. Chem., 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


3. Adipose stem cells from chronic pancreatitis patients improve mouse and human islet survival and function
   Authors: L Song, Z Sun, DS Kim, W Gou, C Strange, H Dong, W Cui, G Gilkeson, KA Morgan, DB Adams, H Wang
   Stem Cell Res Ther, 2017;8(1):192.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


4. Peripheral blood aspirates overexpressing IGF-I via rAAV gene transfer undergo enhanced chondrogenic differentiation processes
   Authors: J Frisch, P Orth, A Rey-Rico, JK Venkatesan, G Schmitt, H Madry, D Kohn, M Cucchiarin
   J. Cell. Mol. Med., 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


5. Chondrogenic Differentiation Processes in Human Bone Marrow Aspirates upon rAAV-Mediated Gene Transfer and Overexpression of the Insulin-Like Growth Factor I
   Authors: Magali Cucchiarin
   Tissue Eng Part A, 2015;21(17):2460-71.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC-P


6. A subset of bone marrow stromal cells regulate ATP-binding cassette gene expression via insulin-like growth factor-I in a leukemia cell line.
   Authors: Benabbou N, Mirshahi P, Bordu C, Faussat A, Tang R, Therwath A, Soria J, Marie J, Mirshahi M
   Int J Oncol, 2014;45(4):1372-80.
   


7. Hypoxia enhances protective effect of placental-derived mesenchymal stem cells on damaged intestinal epithelial cells by promoting secretion of insulin-like growth factor-1.
   Authors: Du, Lili, Yu, Yanqiu, Ma, Haiying, Lu, Xiaomei, Ma, Ling, Jin, Yunan, Zhang, Haipeng
   Int J Mol Sci, 2014;15(2):1983-2002.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


8. IGF-1 promotes the development and cytotoxic activity of human NK cells.
   Authors: Ni, Fang, Sun, Rui, Fu, Binqing, Wang, Fuyan, Guo, Chuang, Tian, Zhigang, Wei, Haiming
   Nat Commun, 2013;4(0):1479.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


9. Benefits of recombinant adeno-associated virus (rAAV)-mediated insulinlike growth factor I (IGF-I) overexpression for the long-term reconstruction of human osteoarthritic cartilage by modulation of the IGF-I axis.
   Authors: Weimer A, Madry H, Venkatesan JK
   Mol. Med., 2012;18(1):346-58.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


10. Growth hormone promotes lymphangiogenesis.
    Authors: Banziger-Tobler NE, Halin C, Kajiya K, Detmar M
    Am. J. Pathol., 2008;173(2):586-97.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development

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