抗HGF Receptor抗体(Anti-HGF Receptor, c-Met, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

目次に戻る

Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human HGF R/c-METGlu25-Thr932Accession # P08581
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human HGF R/c-MET in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross-reactivity with recombinant mouse HGF R is observed.

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human HGF R/c-MET Fc Chimera (Catalog # 358-MT)
Flow Cytometry	2.5 µg/106 cells	MDA‑MB‑231 human breast cancer cell line
Immunohistochemistry	5-15 µg/mL	See below
Blockade of Receptor-ligand Interaction	In a functional ELISA, 0.5-2 µg/mL of this antibody will block 50% of the binding of 5 ng/mL of Recombinant Human HGF (Catalog # 294-HGN) to immobilized Recombinant Human HGF R/c-MET Fc Chimera (Catalog # 358‑MT) coated at 1 µg/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding.
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	5-15 µg/mL	See below
Knockout Validated	HGF R/c‑MET is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in HGF R/c‑MET knockout HeLa cell line.

Immunocytochemistry
	HGF R/c‑MET in HT‑29 and U937 Human Cell Line. HGF R/c‑MET was detected in immersion fixed HT‑29 human colon adenocarcinoma cell line (positive control, left panel) and U937 human histiocytic lymphoma cell line (negative control, right panel) using Goat Anti-Human HGF R/c‑MET Antigen Affinity-purified Polyclonal Antibody (Catalog # AF276) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
	HGF R/c‑MET in Human Liver. HGF R/c-MET was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human HGF R/c-MET Antigen Affinity-purified Polyclonal Antibody (Catalog # AF276) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
	HGF R/c‑MET in Human Skin. HGF R/c-MET was detected in immersion fixed paraffin-embedded sections of human skin using 15 µg/mL Goat Anti-Human HGF R/c-MET Antigen Affinity-purified Polyclonal Antibody (Catalog # AF276) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Knockout Validated
	Western Blot Shows Human HGF R/c‑MET Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HGF R/c-Met knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HGF R/c‑MET Antigen Affinity-purified Polyclonal Antibody (Catalog # AF276) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for HGF R/c‑MET at approximately 150-200 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

目次に戻る

Related Product & Information

Long Name	Hepatocyte Growth Factor Receptor
Entrez Gene IDs	4233 (Human); 17295 (Mouse)
Background	HGF R/c-MET
background_content	Background: HGF R/c-MET HGF R, also known as Met (from N-methyl-N’-nitro-N-nitrosoguanidine induced), is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. HGF R is synthesized as a single chain precursor which undergoes cotranslational proteolytic cleavage. This generates a mature HGF R that is a disulfide-linked dimer composed of a 50 kDa extracellular alpha chain and a 145 kDa transmembrane beta chain (1, 2). The extracellular domain (ECD) contains a seven bladed beta -propeller sema domain, a cysteine-rich PSI/MRS, and four Ig-like E-set domains, while the cytoplasmic region includes the tyrosine kinase domain (3, 4). Proteolysis and alternate splicing generate additional forms of human HGF R which either lack of the kinase domain, consist of secreted extracellular domains, or are deficient in proteolytic separation of the alpha and beta chains (5-7). The sema domain, which is formed by both the alpha and beta chains of HGF R, mediates both ligand binding and receptor dimerization (3, 8). Ligand-induced tyrosine phosphorylation in the cytoplasmic region activates the kinase domain and provides docking sites for multiple SH2-containing molecules (9, 10). HGF stimulation induces HGF R downregulation via internalization and proteasome-dependent degradation (11). In the absence of ligand, HGF R forms non-covalent complexes with a variety of membrane proteins including CD44v6, CD151, EGF R, Fas, Integrin alpha 6/ beta 4, Plexins B1, 2, 3, and MSP R/Ron (12-19). Ligation of one complex component triggers activation of the other, followed by cooperative signaling effects (12-19). Formation of some of these heteromeric complexes is a requirement for epithelial cell morphogenesis and tumor cell invasion (12, 16, 17). Paracrine induction of epithelial cell scattering and branching tubulogenesis results from the stimulation of HGF R on undifferentiated epithelium by HGF released from neighboring mesenchymal cells (20). Genetic polymorphisms, chromosomal translocation, overexpression, and additional splicing and proteolytic cleavage of HGF R have been described in a wide range of cancers (1). Within the ECD, human HGF R shares 86-88% aa sequence identity with canine, mouse, and rat HGF R.

目次に戻る

Citations

1. Tumor and Plasma Met Levels in Non-Metastatic Prostate Cancer
   Authors: Deborah R Kaye
   PLoS ONE, 2016;11(6):e0157130.
   Species: Human
   Sample Type: Plasma
   Application: ELISA detection


2. Beta 1-integrin-c-Met cooperation reveals an inside-in survival signalling on autophagy-related endomembranes
   Authors: Rachel Barrow-McG
   Nat Commun, 2016;7(0):11942.
   


3. Targeting matriptase in breast cancer abrogates tumour progression via impairment of stromal-epithelial growth factor signalling.
   Authors: Zoratti G, Tanabe L, Varela F, Murray A, Bergum C, Colombo E, Lang J, Molinolo A, Leduc R, Marsault E, Boerner J, List K
   Nat Commun, 2015;6(0):6776.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


4. A pharmacodynamic/pharmacokinetic study of ficlatuzumab in patients with advanced solid tumors and liver metastases.
   Authors: Tabernero J, Elez M, Herranz M, Rico I, Prudkin L, Andreu J, Mateos J, Carreras M, Han M, Gifford J, Credi M, Yin W, Agarwal S, Komarnitsky P, Baselga J
   Clin Cancer Res, 2014;20(10):2793-804.
   Species: Human
   Sample Type: Serum
   Application: ELISA detection


5. Internalization of Met requires the co-receptor CD44v6 and its link to ERM proteins.
   Authors: Hasenauer S, Malinger D, Koschut D, Pace G, Matzke A, von Au A, Orian-Rousseau V
   PLoS ONE, 2013;8(4):e62357.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


6. Mutant p53 enhances MET trafficking and signalling to drive cell scattering and invasion.
   Authors: Muller, P A J, Trinidad, A G, Timpson, P, Morton, J P, Zanivan, S, van den Berghe, P V E, Nixon, C, Karim, S A, Caswell, P T, Noll, J E, Coffill, C R, Lane, D P, Sansom, O J, Neilsen, P M, Norman, J C, Vousden, K H
   Oncogene, 2013;32(10):1252-65.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


7. Identification of a pivotal endocytosis motif in c-Met and selective modulation of HGF-dependent aggressiveness of cancer using the 16-mer endocytic peptide.
   Authors: Cho K, Park J, Park C, Lee D, Lee E, Kim D, Kim K, Yoon S, Park Y, Kim E, Cho S, Jang S, Park B, Chi S, Yoo S, Jang M, Kim H, Kim E, Jo K, Park Y
   Oncogene, 2012;32(8):1018-29.
   Species: Human
   Sample Type:
   


8. c-Met-induced epithelial carcinogenesis is initiated by the serine protease matriptase.
   Authors: Szabo R, Rasmussen AL, Moyer AB
   Oncogene, 2011;30(17):2003-16.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


9. Epidermal growth factor receptor regulates MET levels and invasiveness through hypoxia-inducible factor-1alpha in non-small cell lung cancer cells.
   Authors: Xu L, Nilsson MB, Saintigny P, Cascone T, Herynk MH, Du Z, Nikolinakos PG, Yang Y, Prudkin L, Liu D, Lee JJ, Johnson FM, Wong KK, Girard L, Gazdar AF, Minna JD, Kurie JM, Wistuba II, Heymach JV
   Oncogene, 2010;29(18):2616-27.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


10. Dorsal ruffle microdomains potentiate Met receptor tyrosine kinase signaling and down-regulation.
    Authors: Abella JV, Parachoniak CA, Sangwan V, Park M
    J. Biol. Chem., 2010;285(32):24956-67.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC

目次に戻る