抗FGF basic抗体(Anti-FGF Basic, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human FGF basic
Source	Polyclonal Goat IgG
Purification	Protein A or G purified
Specificity	Detects human FGF basic in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human FGF acidic is observed. Neutralizes the biological activity of recombinant human FGF basic. It will also neutralize the biological effects of bovine FGF basic, but it has no effect on recombinant human or bovine FGF acidic.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human FGF basic 157 aa (Catalog # 234-FSE)
Immunohistochemistry	5-15 µg/mL	Immersion fixed paraffin-embedded sections of human ovarian cancer tissue
Neutralization	Measured by its ability to neutralize FGF basic-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. Rizzino, A. et al. (1988) Cancer Res. 48:4266. The Neutralization Dose (ND50) is typically 0.5-1.0 µg/mL in the presence of 0.5 ng/mL Recombinant Human FGF basic 146 aa.

Neutralization

Cell Proliferation Induced by FGF basic and Neutralization by Human FGF basic Antibody. Recombinant Human FGF basic 146 aa (Catalog # 233-FB) stimulates proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF basic 146 aa (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF basic Polyclonal Antibody (Catalog # AB-233-NA). The ND50 is typically 0.5‑1.0 µg/mL.

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Related Product & Information

Long Name	Fibroblast Growth Factor basic
Entrez Gene IDs	2247 (Human); 14173 (Mouse); 281161 (Bovine); 403857 (Canine); 100033955 (Equine)
Background	FGF basic
background_content	Background: FGF basic FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35-60% amino acid conservation. FGF acidic and basic, unlike theother members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway. FGF basic hasbeen isolated from a number of sources, including neural tissue, pituitary, adrenal cortex, corpus luteum, and placenta. This factor contains four cysteine residues,but reduced FGF basic retains full biological activity, indicating that disulfide bonds are not required for this activity. A variety of forms of FGF basic are producedas a result of Nterminal extensions. These extensions affect localization of FGF basic in cellular compartments but do not affect biological activity. Binding of FGFto heparin or cell surface heparan sulfate proteoglycans is necessary for binding of FGF to high affinity FGF receptors. FGF acidic and basic appear to bind to thesame high affinity receptors and show a similar range of biological activities. FGF basic stimulates the proliferation of all cells of mesodermal origin and many cellsof neuroectodermal, ectodermal, and endodermal origin. FGF basic induces neuron differentiation, survival, and regeneration. FGF basic also modulates embryonicdevelopment and differentiation. These observed in vitro functions of FGF basic suggest FGF basic may play a role in vivo in the modulation of such normalprocesses as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, and neuronal function and neural degeneration. Additionally,FGF basic may participate in the production of a variety of pathological conditions resulting from excessive cell proliferation and excessive angiogenesis.

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Citations

1. Reciprocal cellular cross-talk within the tumor microenvironment promotes oncolytic virus activity.
   Authors: Ilkow C, Marguerie M, Batenchuk C, Mayer J, Ben Neriah D, Cousineau S, Falls T, Jennings V, Boileau M, Bellamy D, Bastin D, de Souza C, Alkayyal A, Zhang J, Le Boeuf F, Arulanandam R, Stubbert L, Sampath P, Thorne S, Paramanthan P, Chatterjee A, Strieter R, Burdick M, Addison C, Stojdl D, Atkins H, Auer R, Diallo J, Lichty B, Bell J
   Nat Med, 2015;21(5):530-6.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


2. Tricyclic antidepressant amitriptyline indirectly increases the proliferation of adult dentate gyrus-derived neural precursors: an involvement of astrocytes.
   Authors: Boku, Shuken, Hisaoka-Nakashima, Kazue, Nakagawa, Shin, Kato, Akiko, Kajitani, Naoto, Inoue, Takeshi, Kusumi, Ichiro, Takebayashi, Minoru
   PLoS ONE, 2013;8(11):e79371.
   Species: Rat
   Sample Type: Whole Cells
   Application: Neut


3. Improvement in disability after alemtuzumab treatment of multiple sclerosis is associated with neuroprotective autoimmunity.
   Authors: Jones JL, Anderson JM, Phuah CL, Fox EJ, Selmaj K, Margolin D, Lake SL, Palmer J, Thompson SJ, Wilkins A, Webber DJ, Compston DA, Coles AJ
   Brain, 2010;133(0):2232-47.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


4. Dedicated epithelial recipient cells determine pigmentation patterns.
   Authors: Weiner L, Han R, Scicchitano BM, Li J, Hasegawa K, Grossi M, Lee D, Brissette JL
   Cell, 2007;130(5):932-42.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo

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