抗IL-12抗体(Anti-IL-12, Human, Goat-Poly antibody)

• 2025/12/01

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  [RSD]厳選 抗体＆リコンビナントタンパク質 30％OFFキャンペーン[～2026/02/27] NEW

  期間：2025/12/01 ～2026/02/27

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  R&D Systems, Inc.（RSD）

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑12 heterodimerArg23-Ser219 of p35; Ile23-Ser328 of p40Accession # P29459 (p35) & P29460 (p40)
Source	Polyclonal Goat IgG
Purification	Protein A or G purified
Specificity	Detects human IL-12 in direct ELISAs and Western blots. In these formats, less than 2% cross-reactivity with recombinant mouse IL-12 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human IL‑12 (Catalog # 219-IL)
Neutralization	Measured by its ability to neutralize IL‑12-induced proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) [Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. USA 83:5894]. The Neutralization Dose (ND50) is typically 1-2 µg/mL in the presence of 1 ng/mL Recombinant Human IL‑12.

Neutralization

Cell Proliferation Induced by IL‑12 and Neutralization by Human IL‑12 Antibody. Recombinant Human IL‑12 (Catalog # 219-IL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Human IL‑12 Polyclonal Antibody (Catalog # AB-219-NA). The ND50 is typically 1-2 µg/mL.

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Related Product & Information

Long Name	Interleukin 12
Entrez Gene IDs	3592 (Human); 16159 (Mouse); 84405 (Rat); 403977 (Canine); 493741 (Feline)
Background	IL-12
background_content	Background: IL-12 Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. The p40 subunit of IL-12 has been shown to have extensive amino acid sequence homology to the extracellular domain of the human IL-6 receptor while the p35 subunit shows distant but significant sequence similarity to IL-6, G-CSF, and chicken MGF. These observations have led to the suggestion that IL-12 might have evolved from a cytokine/soluble receptor complex. Human and murine IL-12 share 70% and 60% amino acid sequence homology in their p40 and p35 subunits, respectively. IL-12 apparently shows species specificity with human IL-12 reportedly showing minimal activity in the murine system. IL-12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and natural killer (NK) cells. These effects include inducing production of IFN-gamma and TNF by resting and activated T and NK cells, synergizing with other IFN-gamma  inducers at both the transcriptional and post-transcriptional levels. This interaction induces IFN-gamma  gene expression, enhancing the cytotoxic activity of resting NK and T cells, inducing and synergizing with IL-2 in the generation of lymphokine-activated killer (LAK) cells, acting as a co-mitogen to stimulate proliferation of resting T cells, and inducing proliferation of activated T and NK cells. Current evidence indicates that IL-12, produced by macrophages in response to infectious agents, is a central mediator of the cell-mediated immune response by its actions on the development, proliferation, and activities of TH1 cells. In its role as the initiator of cell-mediated immunity, it has been suggested that IL-12 has therapeutic potential as a stimulator of cell-mediated immune responses to microbial pathogens, metastatic cancers, and viral infections such as AIDS.

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Citations

1. A Novel Bromodomain Inhibitor Reverses HIV-1 Latency through Specific Binding with BRD4 to Promote Tat and P-TEFb Association
   Authors: H Huang, S Liu, M Jean, S Simpson, H Huang, M Merkley, T Hayashi, W Kong, I Rodríguez-, X Zhang, HO Yosief, H Miao, J Que, JJ Kobie, J Bradner, NG Santoso, W Zhang, J Zhu
   Front Microbiol, 2017;8(0):1035.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


2. IL-33 induces IL-9 production in human CD4+ T cells and basophils.
   Authors: Blom L, Poulsen BC, Jensen BM, Hansen A, Poulsen LK
   PLoS ONE, 2011;6(7):e21695.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut

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