抗IL-13抗体(Anti-IL-13, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human IL-13Gly21-Asn132Accession # AAK53823
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human IL-13 in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross‑reactivity with recombinant rhesus monkey IL-13 is observed, approximately 25% cross‑reactivity with recombinant canine IL-13 is observed, and approximately 15% cross‑reactivity with recombinant rat IL-13 is observed and less than 5% cross‑reactivity with recombinant mouse IL-13 and recombinant cotton rat IL-13 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human IL‑13 (Catalog # 213-ILB)
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry	5 µg/106 cells	Human IL-13 transfected NS0 cells fixed with paraformaldehyde and permeabilized with saponin
Neutralization	Measured by its ability to neutralize IL‑13-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.5-3 µg/mL in the presence of 10 ng/mL Recombinant Human IL‑13.

Neutralization

Cell Proliferation Induced by IL‑13 and Neutralization by Human IL‑13 Antibody. Recombinant Human IL‑13 (Catalog # 213-ILB) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑13 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-213-NA). The ND50 is typically 0.5-3 µg/mL.

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Related Product & Information

Long Name	Interleukin 13
Entrez Gene IDs	3596 (Human); 16163 (Mouse); 116553 (Rat); 574325 (Primate)
Background	IL-13
background_content	Background: IL-13 IL-13 is a 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergic asthma and atopy. It is secreted by Th1 and Th2 CD4+ T cells, NK cells, visceral smooth muscle cells, eosinophils, mast cells, and basophils (1-3). IL-13 circulates as a monomer with two internal disulfide bonds that contribute to a bundled four alpha -helix configuration (4, 5). Mature human IL-13 shares 57%, 59%, and 94% amino acid sequence identity with mouse, rat, and rhesus macaque IL-13, respectively. Despite the low homology, it exhibits cross-species activity between human, mouse, and rat (6, 7). IL-13 has diverse activities on numerous cell types (8). On macrophages, IL-13 suppresses the production of pro-inflammatory cytokines and other cytotoxic substances. On B cells, IL-13 induces immunoglobulin class switching to IgE, upregulates the expression of MHC class II, CD71, CD72, and CD23, and costimulates proliferation. IL-13 upregulates IL-6 while downregulating IL-1 and TNF-alpha production by fibroblasts and endothelial cells. IL-13 binds with low affinity to IL-13 R alpha 1, triggering IL-13 R alpha 1 association with IL-4 R alpha. This high affinity receptor complex also functions as the type 2 IL-4 receptor complex (9, 10). Additionally, IL-13 binds with high affinity to IL-13 R alpha 2 which is expressed intracellularly, on the cell surface, and as a soluble molecule (11-14). IL-13 R alpha 2 regulates the bioavailability of both IL-13 and IL-4 and is over-expressed in glioma and several bronchial pathologies (10, 15, 16). Compared to wild type IL-13, the atopy-associated R110Q variant of IL-13 elicits increased responsiveness from eosinophils that express low levels of IL-13 R alpha 2 (17).

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Citations

1. Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells.
   Authors: Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S
   Eur. J. Immunol., 2008;38(4):1012-23.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


2. Histological characteristics and altered expression of interleukins (IL) IL-13 and IL-15 in endometria of levonorgestrel users with different uterine bleeding patterns.
   Authors: Rhoton-Vlasak A, Hardt N
   Fertil. Steril., 2005;83(3):659-65.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


3. Differential expression of interleukins IL-13 and IL-15 in normal ovarian tissue and ovarian carcinomas.
   Authors: Ripley D, Shoup B, Majewski A
   Gynecol. Oncol., 2004;92(3):761-8.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


4. Differential expression of interleukins IL-13 and IL-15 in normal ovarian tissue and ovarian carcinomas.
   Authors: Ripley D, Shoup B, Majewski A
   Gynecol. Oncol., 2004;92(3):761-8.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


5. Human lung myofibroblasts as effectors of the inflammatory process: the common receptor gamma chain is induced by Th2 cytokines, and CD40 ligand is induced by lipopolysaccharide, thrombin and TNF-alpha.
   Authors: Doucet C, Giron-Michel J, Canonica GW, Azzarone B
   Eur. J. Immunol., 2002;32(9):2437-49.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC

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