抗IL-23抗体(Anti-IL-23, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human IL‑23 p19Arg20-Pro189Accession # AAG37232
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human IL‑23 p19 in direct ELISA and Western blot. In direct ELISA, approximately 50% cross-reactivity with recombinant canineIL-23p19, less then 25% cross-reactivity with recombinant feline IL-23p19, less than 5% cross-reactivity with recombinant mouse IL-23p19, recombinant human IL-12p40, and recombinant rat IL-23p40 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Neutralization	Measured by its ability to neutralize IL‑23-induced IL‑17 secretion in mouse splenocytes. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910. The Neutralization Dose (ND50) is typically 0.2-0.8 µg/mL in the presence of 0.75 ng/mL Recombinant Human IL‑23 and 10 ng/mL Recombinant Mouse IL‑2.

Neutralization
	IL‑17 Secretion Induced by IL‑23 and Neutralization by Human IL‑23 Antibody. In the presence of Recombinant Mouse IL‑2 (10 ng/mL, Catalog # 402‑ML), Recombinant Human IL‑23 (Catalog # 1290-IL) stim­ulates IL‑17 secretion in mouse splenocytes in a dose-dependent manner (orange line), as measured by the Mouse IL‑17 Quantikine ELISA Kit (Catalog # M1700). Under these conditions, IL‑17 secretion elicited by Recombinant Human IL‑23 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑23 p19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1716). The ND50 is typically 0.2-0.8 µg/mL.
Western Blot
	Detection of human IL‑23 p19 by Western Blot. Western blot shows lysates of a CHO cell line transfected with human IL-23. A PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IL‑23 p19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1716) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL‑23 p19 at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Related Product & Information

Long Name	Interleukin 23
Entrez Gene IDs	51561 (Human); 83430 (Mouse)
Background	IL-23
background_content	Background: IL-23 Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12. The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Human p19 cDNA encodes a 189 amino acid residue (aa) precursor protein with a putative 19 aa signal peptide and 170 aa mature protein. Human and mouse p19 share 70% aa sequence identity. Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R). IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells. While IL-12 acts on both naïve and memory human T cells, the effects of IL-23 is restricted to memory T cells. In mouse, IL-23 but not IL-12, has also been shown to induce memory T cells to secret IL-17, a potent proinflammatory cytokine. IL-12 and IL-23 can induce IL-12 production from mouse splenic DC of both the CD8- and CD8+ subtypes, however only IL-23 can act directly on CD8+ DC to mediate immunogenic presentation of poorly immunogenic tumor/self peptide.

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Citations

1. Pathogen-induced human TH17 cells produce IFN-gamma or IL-10 and are regulated by IL-1beta.
   Authors: Zielinski CE, Mele F, Aschenbrenner D, Jarrossay D, Ronchi F, Gattorno M, Monticelli S, Lanzavecchia A, Sallusto F
   Nature, 2012;484(7395):514-8.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


2. Human prostate tumor antigen-specific CD8+ regulatory T cells are inhibited by CTLA-4 or IL-35 blockade.
   Authors: Olson B, Jankowska-Gan E, Becker J, Vignali D, Burlingham W, McNeel D
   J Immunol, 2012;189(12):5590-601.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


3. The tachykinins substance P and hemokinin-1 favor the generation of human memory Th17 cells by inducing IL-1beta, IL-23, and TNF-like 1A expression by monocytes.
   Authors: Cunin P, Caillon A, Corvaisier M, Garo E, Scotet M, Blanchard S, Delneste Y, Jeannin P
   J. Immunol., 2011;186(7):4175-82.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


4. Influence of the Notch system in the therapeutic response of American tegumentary leishmaniasis.
   Authors: Rodrigues KM, Oliveira MP, Maretti-Mira AC, Oliveira-Neto MP, Mattos MS, Silva L, Soares DA, Dolci EL, Perico RA, Pirmez C
   Br. J. Dermatol., 2011;164(6):1228-34.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Frozen


5. TLR2-activated human langerhans cells promote Th17 polarization via IL-1beta, TGF-beta and IL-23.
   Authors: Aliahmadi E, Gramlich R, Grutzkau A, Hitzler M, Kruger M, Baumgrass R, Schreiner M, Wittig B, Wanner R, Peiser M
   Eur. J. Immunol., 2009;39(5):1221-30.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


6. IL-23 and IL-12 responses in activated human T cells retrovirally transduced with IL-23 receptor variants.
   Authors: de Paus RA, van de Wetering D, Van Dissel JT
   Mol. Immunol., 2008;45(15):3889-95.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


7. Blockade of TLR9 agonist-induced type I interferons promotes inflammatory cytokine IFN-gamma and IL-17 secretion by activated human PBMC.
   Authors: Meyers JA, Mangini AJ, Nagai T, Roff CF, Sehy D, van Seventer JM
   Cytokine, 2006;35(5):235-46.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: ELISA Development

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