抗IL-13 Receptor α2抗体(Anti-IL-13 Receptor α2, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human IL‑13 R alpha 2Cys22-Leu342Accession # Q14627
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human IL-13 R alpha 2 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse IL-13 R alpha 2 is observed and less than 1% cross-reactivity with recombinant human IL-13 R alpha 1 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.2 µg/mL	See below
Simple Western	10 µg/mL	See below
Flow Cytometry	2.5 µg/106 cells	A375 human melanoma cell line
Immunohistochemistry	5-15 µg/mL	See below
Blockade of Receptor-ligand Interaction	In a functional ELISA, 2-6 µg/mL of this antibody will block 50% of the binding of 100 ng/mL of Recombinant Human IL-13 (Catalog # 213‑ILB) to immobilized Recombinant Human IL-13 R alpha 2 Fc Chimera (Catalog # 614-INS) coated at 4 µg/mL (100 µL/well). At 40 μg/mL, this antibody will block >90% of the binding.
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Western Blot
	Detection of Human IL‑13 R alpha 2 by Western Blot. Western blot shows lysates of human placenta tissue and human testis tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human IL‑13 R alpha 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF146) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL‑13 R alpha 2 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
	IL-13 R alpha 2 in Human Prostate Cancer Tissue. IL‑13 R alpha 2 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human IL‑13 R alpha 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF146) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Simple Western
	Detection of Human IL‑13 R alpha 2 by Simple WesternTM. Simple Western lane view shows lysates of human placenta tissue and human testis tissue, loaded at 0.2 mg/mL. A specific band was detected for IL‑13 R alpha 2 at approximately 40 kDa (as indicated) using 10 µg/mL of Goat Anti-Human IL‑13 R alpha 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF146) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

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Related Product & Information

Long Name	Interleukin 13 Receptor alpha 2
Entrez Gene IDs	3598 (Human); 16165 (Mouse)
Background	IL-13 R alpha 2
background_content	Background: IL-13 R alpha 2 Two type1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha l or NR4, is now referred to as IL-13 R alpha 1. The high affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha l, is now referred to as IL-13 R alpha 2. Human IL-13 R alpha 2 was originally cloned from the Caki-1 human renal carcinoma cell line. The IL-13 R alpha 2 cDNA encodes a 380 amino acid (aa) residue precursor protein with a putative 26 aa residue signal peptide, a 317 residue extracellular domain, a 20 aa residue transmembrane region and a 17 aa residue cytoplasmic tail. Human and mouse IL‑13 R alpha 2 share 59% aa sequence identity. The extracellular domain of IL-13 R alpha 2 is also closely related to that of IL-13 R alpha 1. However, the 17 aa residue cytoplasmic domain of IL-13 R alpha 2 is much shorter than that of IL-13 R alpha 1, suggesting that the two receptors are functionally distinct. IL-13 R alpha 1 has been shown to combine with the IL-4 R to form a high-affinity receptor complex capable of transducing an IL‑13‑dependent proliferative signal. The role of IL-13 R alpha 2 in IL-13 signaling remains to be elucidated. The amino-terminal 27 aa residues of the human and mouse IL‑13 R alpha 2 are nearly identical to that of a soluble mouse IL-13 binding protein purified from mouse serum and urine.

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Citations

1. IL-13 receptors as possible therapeutic targets in diffuse intrinsic pontine glioma
   Authors: NE Berlow, MN Svalina, MJ Quist, TP Settelmeye, V Zherebitsk, M Kogiso, L Qi, Y Du, CE Hawkins, E Hulleman, XN Li, SH Gultekin, C Keller
   PLoS ONE, 2018;13(4):e0193565.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


2. IL-4 primes the dynamics of breast cancer progression via DUSP4 inhibition
   Authors: M Gaggianesi, A Turdo, A Chinnici, E Lipari, T Apuzzo, A Benfante, I Sperduti, S Di Franco, S Meraviglia, E Lo Presti, F Dieli, V Caputo, G Militello, S Vieni, G Stassi, M Todaro
   Cancer Res., 2017;0(0):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. Cigarette smoke extracts induce overexpression of the proto-oncogenic gene interleukin-13 receptor ?2 through activation of the PKA-CREB signaling pathway to trigger malignant transformation of lung vascular endothelial cells and angiogenesis
   Authors: Mei Meng
   Cell. Signal, 2016;0(0):.
   


4. IL-13R?2 uses TMEM219 in chitinase 3-like-1-induced signalling and effector responses
   Nat Commun, 2016;7(0):12752.
   Species: Human
   Sample Type: Cell Lysates
   Application: IP


5. Role of IL13RA2 in Sunitinib Resistance in Clear Cell Renal Cell Carcinoma.
   Authors: Shibasaki N, Yamasaki T, Kanno T, Arakaki R, Sakamoto H, Utsunomiya N, Inoue T, Tsuruyama T, Nakamura E, Ogawa O, Kamba T
   PLoS ONE, 2015;10(6):e0130980.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC - Paraffin embedded


6. Expression of surfactant protein D in airways of asthmatics and interleukin-13 modulation of surfactant protein D in human models of airway epithelium.
   Authors: Xu J, Singhera G, Dorscheid D
   Respir Res, 2015;16(0):26.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


7. IL-13Ralpha2 mediates PNR-induced migration and metastasis in ERalpha-negative breast cancer.
   Authors: Zhao Z, Wang L, Xu W
   Oncogene, 2015;34(12):1596-607.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


8. Absence of a role for interleukin-13 in inflammatory bowel disease.
   Authors: Biancheri P, Di Sabatino A, Ammoscato F, Facciotti F, Caprioli F, Curciarello R, Hoque S, Ghanbari A, Joe-Njoku I, Giuffrida P, Rovedatti L, Geginat J, Corazza G, MacDonald T
   Eur J Immunol, 2014;44(2):370-85.
   


9. Cytokine induction of VCAM-1 but not IL13Ralpha2 on glioma cells: a tale of two antibodies.
   Authors: Mahadev V, Starr R, Wright S, Martinez C, Jensen M, Barish M, Forman S, Brown C
   PLoS ONE, 2014;9(5):e95123.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


10. Matrix metalloproteinase 8 contributes to solubilization of IL-13 receptor alpha2 in vivo.
    Authors: Chen W, Tabata Y, Gibson AM, Daines MO, Warrier MR, Wills-Karp M, Hershey GK
    J. Allergy Clin. Immunol., 2008;122(3):625-32.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

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