抗IL-18 Binding Protein-a抗体(Anti-IL-18 Binding Protein-a, Human, Goat-Poly antibody)

掲載日情報:2019/10/21 現在Webページ番号:26763

IL-18 Binding Protein-aに対する抗体(Anti-IL-18 Binding Protein-a, Human, Goat-Poly )です。
本製品は研究用です。研究用以外には使用できません。

価格

[在庫・価格 :2025年02月14日 19時35分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
詳細 商品名
  • 商品コード
  • メーカー
  • 包装
  • 価格
  • 在庫
  • 法規制等
納期 文献数
Anti-IL-18 Binding Protein-a, Human, Goat-Poly
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 2
  • 使用文献
  • メーカーサイト
  • お問い合わせ
説明文
別名:IL18 BPa
Genbank No: 10068
Protein Accession No: O95998
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Goat クラス IgG 標識 Unlabeled
交差性 Human 適用 ELISA,Neutralising,Western Blot
クロナリティ Polyclonal フォーマット 性状 Antigen Affinity Purified 吸収処理
掲載カタログ

製品記事
関連記事
Anti-Human IL-18 BPa Affinity Purified Polyclonal Ab
2~3週間 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 0
  • メーカーサイト
  • お問い合わせ
説明文
※受注発注品。形状:溶液または凍結乾燥
別名:IL18 BPa
Genbank No: 10068
Protein Accession No: O95998
別包装品 別包装品あり
法規制等
保存条件 法規備考
抗原種 免疫動物 Goat クラス IgG 標識 Unlabeled
交差性 Human 適用 ELISA,Neutralising,Western Blot
クロナリティ Polyclonal フォーマット 性状 Antigen Affinity Purified 吸収処理
掲載カタログ

製品記事 Helper T Cells Marker Antibodies
使いっきり抗体
関連記事

[在庫・価格 :2025年02月14日 19時35分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-IL-18 Binding Protein-a, Human, Goat-Poly

文献数: 2

説明文 別名:IL18 BPa
Genbank No: 10068
Protein Accession No: O95998
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Goat
交差性 Human 適用 ELISA,Neutralising,Western Blot
標識 Unlabeled 性状 Antigen Affinity Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事
関連記事

Anti-Human IL-18 BPa Affinity Purified Polyclonal Ab

文献数: 0

説明文 ※受注発注品。形状:溶液または凍結乾燥
別名:IL18 BPa
Genbank No: 10068
Protein Accession No: O95998
別包装品 別包装品あり
法規制等
保存条件 法規備考
抗原種 免疫動物 Goat
交差性 Human 適用 ELISA,Neutralising,Western Blot
標識 Unlabeled 性状 Antigen Affinity Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 Helper T Cells Marker Antibodies
使いっきり抗体
関連記事



目次に戻る

Product Details

Species ReactivityHuman
LabelUnconjugated
ImmunogenMouse myeloma cell line NS0-derived recombinant human IL-18 BPaThr31-Gly194Accession # O95998
SourcePolyclonal Goat IgG
PurificationAntigen Affinity-purified
SpecificityDetects human IL‑18 BPa in direct ELISAs and Western blots. In Western blots, less than 1% cross-reactivity with recombinant mouse (rm) IL‑18 BPc and rmIL-18 BPd is observed.


目次に戻る

Applications and Data

 Recommended
Concentration
Sample
Western Blot0.5 µg/mLSee below
ELISA

This antibody functions as an ELISA detection antibody when paired with Rabbit Anti-Human IL‑18/IL‑1F4 Monoclonal Antibody (Catalog # MAB91244).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human IL-18/IL-18 BPa Complex DuoSet ELISA Kit (Catalog # DY8936-05) for convenient development of a sandwich ELISA or the Human IL-18 BPa Quantikine ELISA Kit (Catalog # DBP180) for a complete optimized ELISA.

NeutralizationMeasured by its ability to neutralize IL‑18 BPa inhibition of IL‑18/IL‑1F4-induced IFN‑ gamma secretion in the KG‑1 human acute myelogenous leukemia cell line. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 0.1 µg/mL Recombinant Human IL‑18 BPa Fc Chimera, 40 ng/mL Recombinant Human IL‑18/IL‑1F4, and 20 ng/mL Recombinant Human TNF‑ alpha.


Western Blot
Detection of Human IL‑18 BPa by Western Blot.
Western blot shows conditioned media from HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 50 ng/mL Recombinant Human IFN‑ gamma (Catalog # 285‑IF) and 40 ng/mL Recombinant Human TNF‑ alpha (Catalog # 210‑TA) for 48 hours. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human IL‑18 BPa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF119) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL‑18 BPa at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Neutralization
IL‑18 BPa Inhibition of IL‑18/IL‑1F4-induced IFN‑ gamma Secretion and Neutralization by Human IL‑18 BPa Antibody.
Recombinant Human IL‑18 BPa Fc Chimera (Catalog # 119-BP) inhibits Recombinant HumanIL‑18/IL‑1F4 induced IFN‑ gamma secretion in the KG‑1 human acute myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the Human IFN‑ gamma Quantikine ELISA Kit (Catalog # DIF50). Inhibition of Recombinant Human IL‑18/IL‑1F4 (40 ng/mL) activity elicited by Recombinant Human IL‑18 BPa Fc Chimera (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑18 BPa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF119). The ND50 is typically 1-5 µg/mL in the presence of Recombinant Human TNF‑ alpha (20 ng/mL).
ELISA
Human IL‑18/IL-18 BPa Complex ELISA Standard Curve.
Recombinant Human IL‑18/IL-18 BPa complex protein was serially diluted 2-fold and captured by Rabbit Anti-Human IL‑18/IL‑1F4 Monoclonal Antibody (Catalog # MAB91244) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑18 BPa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF119) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).


目次に戻る

Related Product & Information

Long NameInterleukin 18 Binding Protein Isoform a
Entrez Gene IDs10068 (Human)
BackgroundIL-18 BPa
background_contentBackground:
IL-18 BPa
Interleukin 18 binding protein (IL-18 BP) is a secreted glycoprotein, which functions as an IL-18 antagonist by binding to IL-18 and blocking its biological activity. IL‑18 BP bears no amino acid sequence homology to the membrane-associated IL-18 and IL-1 receptor proteins. The gene for human IL-18 BP has been localized to chromosome 11q13. It encodes for at least four isoforms by alternative splicing. The IL-18 BP isoforms a and c each contain one immunoglobulin (Ig)-like C2-type domain while isoforms b and d lack a complete Ig domain. The complete Ig domain has been shown to be essential to the binding and neutralizing properties of the binding proteins. Two isoforms of mouse IL18 BP (c and d) containing the complete Ig domain have also been isolated and shown to neutralize IL-18 bioactivity. Human and mouse IL-18 BPs share approximately 61% amino acid sequence identity. Several poxviruses also encode proteins with sequence similarity to the human and mouse IL-18 BP. Viral IL-18 BPs have been shown to bind and inhibit IL-18 responses and may be involved in modulating host immune responses. The expression of IL-18 BP is markedly up-regulated by IFN-gamma, suggesting that IL-18 activity is modulated by a negative feedback mechanism mediated by IL-18 BP.


目次に戻る

Citations

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
  1. Differentiation of human SH-SY5Y neuroblastoma cells by all-trans retinoic acid activates the interleukin-18 system.
    Authors: Sallmon H, Hoene V, Weber SC, Dame C
    J. Interferon Cytokine Res., 2010;30(2):55-8.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB

  2. Generation and characterization of mice transgenic for human IL-18-binding protein isoform a.
    Authors: Fantuzzi G, Banda NK, Guthridge C, Vondracek A, Kim SH, Siegmund B, Azam T, Sennello JA, Dinarello CA, Arend WP
    J. Leukoc. Biol., 2003;74(5):889-96.
    Species: Human
    Sample Type: Serum
    Application: ELISA Development



目次に戻る

お問い合わせ先

(テクニカルサポート 試薬担当)

reagent@funakoshi.co.jp

製品情報は掲載時点のものですが、価格表内の価格については随時最新のものに更新されます。お問い合わせいただくタイミングにより製品情報・価格などは変更されている場合があります。
表示価格に、消費税等は含まれていません。一部価格が予告なく変更される場合がありますので、あらかじめご了承下さい。