抗Neogenin抗体(Anti-Neogenin, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse NeogeninAla42-Ile1033 (Asp442-Leu461 del)Accession # NP_032710
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human and mouse Neogenin in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant mouse DCC is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.5 µg/mL	See below
Simple Western	10 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below
Blockade of Receptor-ligand Interaction	In a functional ELISA, 2.5-10 µg/mL of this antibody will block 50% of the binding of 200 ng/mL of Recombinant Chicken Netrin-1 (Catalog # 128‑N1) to immobilized Recombinant Mouse Neogenin Fc Chimera (Catalog # 1079‑NE) coated at 5 µg/mL (100 µL/well). At 300 μg/mL, this antibody will block >90% of the binding.

Western Blot
	Detection of Human and Mouse Neogenin by Western Blot. Western blot shows lysates of 3T3‑L1 mouse embryonic fibroblast adipose-like cell line, Jurkat human acute T cell leukemia cell line, and Hepa 1‑6 mouse hepatoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Neogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1079) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Neogenin at approximately 230 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
	Neogenin in Mouse Embryo. Neogenin was detected in perfusion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Human/Mouse Neogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1079) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing neurons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Simple Western
	Detection of Mouse Neogenin by Simple WesternTM. Simple Western lane view shows lysates of beta TC‑6 mouse beta cell insulinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Neogenin at approximately 207 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse Neogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1079) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Related Product & Information

Entrez Gene IDs	4756 (Human); 18007 (Mouse)
Background	Neogenin
background_content	Background: Neogenin Neogenin (NEO) is a type I transmembrane protein that is crucial for axonal guidance and neuronal migration. It is also involved in regulating differentiation programs in many embryonic and adult tissues (1). Mouse NEO is widely expressed in adult tissues and is expressed throughout the mid to late stages of gestation, in both neuronal and non-neuronal tissues. It is a member of the immunoglobulin (Ig) superfamily and is closely related to deleted in colorectal cancer (DCC). Mouse NEO cDNA encodes a 1493 amino acid residue (aa) precursor with a putative 36 aa signal peptide, a 1100 aa extracellular domain with six Ig-like C2 type domains and three fibronectin type III domains, a 21 aa transmembrane domain, and a 345 aa cytoplasmic domain. At least five isoforms are produced in mice by alternative splicing. Mouse NEO shares 96%, 93%, and 86% aa sequence identity with rat, human, and chicken NEO, respectively. It also has 46% and 29% sequence homology with mouse DCC and C. elegans UNC40, a homolog of DCC. NEO and DCC, together with the UNC5 family of type I transmembrane proteins, are receptors for the netrin/UNC6 family of laminin-related bifunctional guidance molecules that both attract some axons and repel others (2, 3). In mouse, at least five netrins (netrin‑1,‑3, -4, G1, and G2) have been identified (3-5). Mouse netrin-1 and netrin-3 have been shown to be ligands for mouse NEO.

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Citations

1. Tbr1 instructs laminar patterning of retinal ganglion cell dendrites
   Authors: J Liu, JDS Reggiani, MA Laboulaye, S Pandey, B Chen, JLR Rubenstein, A Krishnaswa, JR Sanes
   Nat. Neurosci., 2018;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


2. Effect of Erythropoietin, Iron Deficiency and Iron Overload on Liver Matriptase-2 (TMPRSS6) Protein Content in Mice and Rats.
   Authors: Frydlova J, Prikryl P, Truksa J, Falke L, Du X, Gurieva I, Vokurka M, Krijt J
   PLoS ONE, 2015;11(2):e0148540.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


3. The axonal guidance receptor neogenin promotes acute inflammation.
   Authors: Konig K, Gatidou D, Granja T, Meier J, Rosenberger P, Mirakaj V
   PLoS ONE, 2012;7(3):e32145.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


4. Genetic analysis of DSCAM's role as a Netrin-1 receptor in vertebrates.
   Authors: Palmesino E, Haddick PC, Tessier-Lavigne M, Kania A
   J. Neurosci., 2012;32(2):411-6.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


5. Netrin-1 overexpression protects kidney from ischemia reperfusion injury by suppressing apoptosis.
   Authors: Wang W, Reeves WB, Pays L, Mehlen P, Ramesh G
   Am. J. Pathol., 2009;175(3):1010-8.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


6. Interaction of amyloid precursor protein with contactins and NgCAM in the retinotectal system.
   Authors: Osterfield M, Egelund R, Young LM, Flanagan JG
   Development, 2008;135(6):1189-99.
   Species: Chicken
   Sample Type: Tissue Homogenates
   Application: WB


7. Selective binding of RGMc/hemojuvelin, a key protein in systemic iron metabolism, to BMP-2 and neogenin.
   Authors: Kuns-Hashimoto R, Kuninger D, Nili M, Rotwein P
   Am. J. Physiol., Cell Physiol., 2008;294(4):C994-C1003.
   Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
   Sample Type: Whole Cells
   Application: Neut


8. Netrin-4 inhibits angiogenesis via binding to neogenin and recruitment of Unc5B.
   Authors: Lejmi E, Leconte L, Pedron-Mazoyer S, Ropert S, Raoul W, Lavalette S, Bouras I, Feron JG, Maitre-Boube M, Assayag F, Feumi C, Alemany M, Jie TX, Merkulova T, Poupon MF, Ruchoux MM, Tobelem G, Sennlaub F, Plouet J
   Proc. Natl. Acad. Sci. U.S.A., 2008;105(34):12491-6.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB

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